The Effect of Phenotypic Bias on Lineup Construction Fairness

There is converging evidence that people make inferences about others’ culpability and deservingness of punishment based on whether they express more of the African phenotype (e.g., darker skin, wider nose, thicker lips; Blair, Judd, & Chapleau, 2004; Eberhardt Goff, Purdie & Davies, 2004; Kahn & Davies, 2011). What is less clear is whether facial features that are phenotypically related to particular racial groups play a role in the mistaken identification of innocent Black suspects. Eyewitness descriptions lack detail with regard to racial phenotypes (Fahsing, Ask & Granhag, 2004; Nicholson & Kovera, 2013). Without descriptions containing phenotypic features to use when choosing fillers (i.e., lineup members who are not the suspect) in lineups or photo arrays, it is possible that lineup constructors will choose fillers that do not match the phenotype expression of a suspect, increasing the risk that the suspect will stand out from among the other members of the lineup and that the lineup will be unfair. It is also possible that the racial stereotype associated with the crime under investigation may influence the phenotype expression of fillers chosen by lineup constructors (Kleider, Cavarak & Knuycky, 2012; Knuycky, Kleider & Cavarak, 2014; Osborne & Davies, 2012). This study employed four phases to test whether phenotypic bias affects lineup fairness. Phase 1 and 2 developed the stimulus materials. Phase 3 had participants construct lineups in a 2 (Construction method: match-to-suspect or match-to-description) x 3 (Crime type: stereotypically Black, stereotypically White or blind) x 2 (Suspect level of African phenotype expression: high or low). Phase 4 measured the fairness of the lineups created in Phase 3 to assess the potential effect of phenotypic bias on mock witness lineup identifications. I found that descriptions of out-group members are indeed vague and devoid of descriptors specific to phenotypically African features (Phase 2). In addition, lineups constructed using the match-to-suspect method of lineup construction consisted of fillers with similar African phenotype expression ratings as the suspect’s rating, but lineups created using the match-to-description method had fillers with African phenotype expression ratings that did not match the suspect’s ratings (Phase 3). Mock witnesses’ lineup up identifications were affected by phenotypic bias, regardless of construction condition, but lineups containing suspects with low African phenotype expression were biased to the advantage of the suspect and lineups containing suspects with high African phenotype were biased to the disadvantage of the suspect (Phase 4). Given these findings, the recommended procedural safeguards may not be sufficient in protecting Black suspects with high African phenotype expression from the prejudicial effects of phenotypic bias

An investigation of the traffic affecting the retail structure of the Claremont business centre

Decentralisation of the cities throughout the world linked with the added mobility of the community due to the automobile has resulted in the phenomenal growth of the old satellite villages which were normally located at transportation nodes. To these new growth points must be added the true regional shopping centre set in the country and surrounded by its sea of parked vehicles. The regional centre has, as yet, not developed in South Africa but the phenomenon of decentralisation has stimulated the growth in certain of the old established local shopping areas surrounding the larger cities

Ghosting from the workplace: The impact of feedback (or lack thereof) on applicants’ psychological needs satisfaction

Background: Applying for a job can be a stressful experience. When a potential employer chooses to not move forward with a candidate, they can either directly tell them or cease communication altogether—known as ghosting. Ghosting has harmful consequences, such as lower basic psychological needs satisfaction than direct rejection, and there has been an apparent increase in this phenomenon by potential employers. The purpose of this study was to examine the effect of being ghosted after submitting a hypothetical job application. Methods: An international sample of unemployed people (N = 554) completed an online job application task and were randomly assigned to receive no (i.e., ghosted), impersonal, or personal feedback. Results: While our manipulation was perceived as intended, there was not an effect of condition on needs satisfaction. However, exploratory analyses suggest that ghosted participants have greater self-esteem and control than those that received personal feedback. Thus, the effect of being ghosted during a job application may not be as clear cut as previously thought. Conclusion: Although the current study did not find evidence that applicants feel worse after being ghosted than being directly rejected, such an experience can still hurt, and more research is needed to understand the long-term effects of being ghosted by a potential employer

Antibody profiling and predictive modeling discriminate between Kaposi sarcoma and asymptomatic KSHV infection

Protein-level immunodominance patterns against Kaposi sarcoma-associated herpesvirus (KSHV), the aetiologic agent of Kaposi sarcoma (KS), have been revealed from serological probing of whole protein arrays, however, the epitopes that underlie these patterns have not been defined. We recently demonstrated the utility of phage display in high-resolution linear epitope mapping of the KSHV latency-associated nuclear antigen (LANA/ORF73). Here, a VirScan phage immunoprecipitation and sequencing approach, employing a library of 1,988 KSHV proteome-derived peptides, was used to quantify the breadth and magnitude of responses of 59 sub-Saharan African KS patients and 22 KSHV-infected asymptomatic individuals (ASY), and ultimately to support an application of machine-learning-based predictive modeling using the peptide-level responses. Comparing anti-KSHV antibody repertoire revealed that magnitude, not breadth, increased in KS. The most targeted epitopes in both KS and ASY were in the immunodominant proteins, notably, K8.129−56 and ORF65140-168, in addition to LANA. Finally, using unbiased machine-learning-based predictive models, reactivity to a subset of 25 discriminative peptides was demonstrated to successfully classify KS patients from asymptomatic individuals. Our study provides the highest resolution mapping of antigenicity across the entire KSHV proteome to date, which is vital to discern mechanisms of viral pathogenesis, to define prognostic biomarkers, and to design effective vaccine and therapeutic strategies. Future studies will investigate the diagnostic, prognostic, and therapeutic potential of the 25 discriminative peptides

HIV-1 subtypes and drug resistance mutations among female sex workers varied in different cities and regions of the Democratic Republic of Congo

Background Complex mosaic structures of HIV-1 were found in the Democratic Republic of Congo (DRC). Currently, there is limited information on the circulating HIV-1 strains, the distribution of these strains and antiretroviral (ART) resistant viruses in different regions of the country, and the HIV-1 strains harbored by the high-risk groups like female sex workers (FSW) reported to be the source of recombinant and ART resistant viruses. Methods Dried Blood Spots (DBS), collected from 325 infected FSWs in ten cities from 2012 DRC HIV/STI Integrated Biological and Behavioral Surveillance Survey, were tested for HIV-1 genotypes and antiretroviral resistance mutations. Regional segregation of HIV-1 clades was detected using phylogenetics. The significance for differences in HIV-1 subtype and drug resistance mutations were evaluated using Chi-square tests. Results There were 145 (env) and 93 (pol) sequences analyzed. Based on env sequences, the predominant subtype was A1 (44%), and recombinants as defined pol sequences comprised 35% of the total sample. Paired sequences of pol and env from DRC FSW revealed mosaic recombinant in 54% of the sequences. Distinct geographic distributions of different HIV-1 subtypes and recombinants were observed. Subtype A1 was prevalent (40%) in Goma located in the East and significantly higher than in Mbuji-Mayi (p\u3c0.05) in the South-centra

Longitudinal Variations in Antibody Responses against SARS-CoV-2 Spike Epitopes upon Serial Vaccinations

The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) impacted healthcare, the workforce, and worldwide socioeconomics. Multi-dose mono- or bivalent mRNA vaccine regimens have shown high efficacy in protection against SARSCoV- 2 and its emerging variants with varying degrees of efficacy. Amino acid changes, primarily in the receptor-binding domain (RBD), result in selection for viral infectivity, disease severity, and immune evasion. Therefore, many studies have centered around neutralizing antibodies that target the RBD and their generation achieved through infection or vaccination. Here, we conducted a unique longitudinal study, analyzing the effects of a three-dose mRNA vaccine regimen exclusively using the monovalent BNT162b2 (Pfizer/BioNTech) vaccine, systematically administered to nine previously uninfected (naïve) individuals. We compare changes in humoral antibody responses across the entire SARS-CoV-2 spike glycoprotein (S) using a high-throughput phage display technique (VirScan). Our data demonstrate that two doses of vaccination alone can achieve the broadest and highest magnitudes of anti-S response. Moreover, we present evidence of novel highly boosted non-RBD epitopes that strongly correlate with neutralization and recapitulate independent findings. These vaccine-boosted epitopes could facilitate multi-valent vaccine development and drug discovery

Employing Respondent Driven Sampling (RDS) to recruit people who inject drugs (PWID) and other hard-to-reach populations during COVID-19: Lessons learned

Background: Respondent Driven Sampling (RDS) is an effective sampling strategy to recruit hard-to-reach populations but the impact of the COVID-19 pandemic on the use of this strategy in the collection of data involving human subjects, particularly among marginalized and vulnerable populations, is not known. Based on an ongoing study using RDS to recruit and study the interactions between HIV infection, injection drug use, and the microbiome in Puerto Rico, this paper explores the eectiveness of RDS during the pandemic and provided potential strategies that could improve recruitment and data collection. Results: RDS was employed to evaluate its effectiveness in recruiting a group of people who inject drugs (PWID) and controls (N = 127) into a study in the midst of the COVID-19 pandemic. The participants were distributed among three subsets: 15 were HIV+ and PWID, 58 were HIV- PWID, and 54 were HIV+ and not PWID. Findings: Results showthat recruitment through peer networks using RDS was possible across all sub-groups. Yet, while those in the HIV+ PWID sub-group managed to recruit from other-sub groups of HIV- PWID and HIV+, this occurred at a lower frequency. Conclusion: Despite the barriers introduced by COVID-19, it is clear that even in this environment, RDS continues to play a powerful role in recruiting hard-to-reach populations. Yet, more attention should be paid at how future pandemics, natural disasters, and other big events might affect RDS recruitment of vulnerable and hard-to-reach populations

Viral Epitope Scanning Reveals Correlation between Seasonal HCoVs and SARS-CoV-2 Antibody Responses among Cancer and Non-Cancer Patients

Seasonal coronaviruses (HCoVs) are known to contribute to cross-reactive antibody (Ab) responses against SARS-CoV-2. While these responses are predictable due to the high homology between SARS-CoV-2 and other CoVs, the impact of these responses on susceptibility to SARS-CoV-2 infection in cancer patients is unclear. To investigate the influence of prior HCoV infection on anti- SARS-CoV-2 Ab responses among COVID-19 asymptomatic individuals with cancer and controls without cancers, we utilized the VirScan technology in which phage immunoprecipitation and sequencing (PhIP-seq) of longitudinal plasma samples was performed to investigate high-resolution (i.e., epitope level) humoral CoV responses. Despite testing positive for anti-SARS-CoV-2 Ab in the plasma, a majority of the participants were asymptomatic for COVID-19 with no prior history of COVID-19 diagnosis. Although the magnitudes of the anti-SARS-CoV-2 Ab responses were lower in individuals with Kaposi sarcoma (KS) compared to non-KS cancer individuals and those without cancer, the HCoV Ab repertoire was similar between individuals with and without cancer independent of age, sex, HIV status, and chemotherapy. The magnitudes of the anti-spike HCoV responses showed a strong positive association with those of the anti-SARS-CoV-2 spike in cancer patients, and only a weak association in non-cancer patients, suggesting that prior infection with HCoVs might play a role in limiting SARS-CoV-2 infection and COVID-19 disease severity

Expressions 2013

https://openspace.dmacc.edu/expressions/1027/thumbnail.jp