Eurycea tridentifera

Number of Pages: 2Integrative BiologyGeological Science

Pituophis melanoleucus

Number of Pages: 8Integrative BiologyGeological Science

A single species of mangrove monitor (Varanus) occupies Ambon, Seram, Buru and Saparua, Moluccas, Indonesia

According to current literature the islands of the central Moluccan region harbor at least three species of monitor lizards. This suggests similar patterns of species richness to the northern Moluccas and could imply significant taxonomic and ecological complexity throughout the Moluccan region. Field investigations in habitats from sea level up to 300 m elevation failed to locate more than one widespread species, by definition referable to Varanus indicus (type locality Ambon). Reassessments of records for other species of mangrove monitors show that these can either be attributed to taxonomic mis-identifications or to colonial-era specimens lacking reliable collection data. We test Principal Components Analysis of scalation characters as a diagnostic tool for some of the island populations and species within the Varanus indicus group.</p

Usefulness of gene expression profiling of bronchoalveolar lavage cells in acute lung allograft rejection.

BackgroundChronic lung allograft dysfunction (CLAD) is the main limitation to long-term survival after lung transplantation. Because effective therapies are lacking, early identification and mitigation of risk factors is a pragmatic approach to improve outcomes. Acute cellular rejection (ACR) is the most pervasive risk factor for CLAD, but diagnosis requires transbronchial biopsy, which carries risks. We hypothesized that gene expression in the bronchoalveolar lavage (BAL) cell pellet (CP) could replace biopsy and inform on mechanisms of CLAD.MethodsWe performed RNA sequencing on BAL CPs from 219 lung transplant recipients with A-grade ACR (n = 61), lymphocytic bronchiolitis (n = 58), infection (n = 41), or no rejection/infection (n = 59). Differential gene expression was based on absolute fold difference &gt;2.0 and Benjamini-adjusted p-value ≤0.05. We used the Database for Annotation, Visualization and Integrated Discovery Bioinformatics Resource for pathway analyses. For classifier modeling, samples were randomly split into training (n = 154) and testing sets (n = 65). A logistic regression model using recursive feature elimination and 5-fold cross-validation was trained to optimize area under the curve (AUC).ResultsDifferential gene expression identified 72 genes. Enriched pathways included T-cell receptor signaling, natural killer cell-mediated cytotoxicity, and cytokine-cytokine receptor interaction. A 4-gene model (AUC = 0.72) and classification threshold defined in the training set exhibited fair performance in the testing set; accuracy was 76%, specificity 82%, and sensitivity 60%. In addition, classification as ACR was associated with worse CLAD-free survival (hazard ratio = 2.42; 95% confidence interval = 1.29-4.53).ConclusionsBAL CP gene expression during ACR is enriched for immune response pathways and shows promise as a diagnostic tool for ACR, especially ACR that is a precursor of CLAD

The SAMI Galaxy Survey: mass-kinematics scaling relations

We use data from the Sydney-AAO Multi-object Integral-field spectroscopy (SAMI) Galaxy Survey to study the dynamical scaling relation between galaxy stellar mass $M_*$ and the general kinematic parameter $S_K = \sqrt{K V_{rot}^2 + \sigma^2}$ that combines rotation velocity $V_{rot}$ and velocity dispersion $\sigma$. We show that the $\log M_* - \log S_K$ relation: (1)~is linear above limits set by properties of the samples and observations; (2)~has slightly different slope when derived from stellar or gas kinematic measurements; (3)~applies to both early-type and late-type galaxies and has smaller scatter than either the Tully-Fisher relation ($\log M_* - \log V_{rot}$) for late types or the Faber-Jackson relation ($\log M_* - \log\sigma$) for early types; and (4)~has scatter that is only weakly sensitive to the value of $K$, with minimum scatter for $K$ in the range 0.4 and 0.7. We compare $S_K$ to the aperture second moment (the `aperture velocity dispersion') measured from the integrated spectrum within a 3-arcsecond radius aperture ($\sigma_{3^{\prime\prime}}$). We find that while $S_{K}$ and $\sigma_{3^{\prime\prime}}$ are in general tightly correlated, the $\log M_* - \log S_K$ relation has less scatter than the $\log M_* - \log \sigma_{3^{\prime\prime}}$ relation.Comment: 14 pages, 8 figures, Accepted 2019 May 22. Received 2019 May 18; in original form 2019 January

CRIg-expressing peritoneal macrophages are associated with disease severity in patients with cirrhosis and ascites

Infections are an important cause of morbidity and mortality in patients with decompensated cirrhosis and ascites. Hypothesizing that innate immune dysfunction contributes to susceptibility to infection, we assessed ascitic fluid macrophage phenotype and function. The expression of complement receptor of the immunoglobulin superfamily (CRIg) and CCR2 defined two phenotypically and functionally distinct peritoneal macrophage subpopulations. The proportion of CRIg(hi) macrophages differed between patients and in the same patient over time, and a high proportion of CRIg(hi) macrophages was associated with reduced disease severity (model for end-stage liver disease) score. As compared with CRIg(lo) macrophages, CRIg(hi) macrophages were highly phagocytic and displayed enhanced antimicrobial effector activity. Transcriptional profiling by RNA sequencing and comparison with human macrophage and murine peritoneal macrophage expression signatures highlighted similarities among CRIg(hi) cells, human macrophages, and mouse F4/80(hi) resident peritoneal macrophages and among CRIg(lo) macrophages, human monocytes, and mouse F4/80lo monocyte-derived peritoneal macrophages. These data suggest that CRIg(hi) and CRIg(lo) macrophages may represent a tissue-resident population and a monocytederived population, respectively. In conclusion, ascites fluid macrophage subset distribution and phagocytic capacity is highly variable among patients with chronic liver disease. Regulating the numbers and/or functions of these macrophage populations could provide therapeutic opportunities in cirrhotic patients

Deepwater Horizon Oil Spill Exposure, Industry Sector, and Child Health

The historic 2010 BP Deepwater Horizon Oil Spill (DHOS) led to public distress regarding potential impacts on children in nearby Gulf Coast communities. Using a community-based South Louisiana panel study of households with children, we examined the effect of fishing industry employment on changes in a subjective measure of general child health and whether economic and physical DHOS exposures played a mediating role. Fishing industry employment had a negative effect on child health compared to other industries. Economic exposure and physical exposure both mediated the effects of the fishing industry on child health, with economic exposure mediating a larger share (49.3%) of the relationship compared to physical exposure (40.5%). The importance of economic oil spill exposure in these findings highlights the significance of social determinants of health at the intersection of disasters and child vulnerability