Does Handedness Impact Pulmonary Measures during Pickleball?

Pickleball is a racquet sport that originated in the 1960s. Due to its beginner-friendly nature, it attracts players of all ages and fitness levels. Despite becoming the most quickly growing sport in the nation, it is still underresearched. The sport’s physiological demands based on pulmonary measures, and whether the demands differ by handedness, are not fully understood. PURPOSE: The purpose of this study was to analyze and assess whether differences were evident in performance and physiological responses in players when using the dominant (DH) and nondominant hand (NDH) during pickleball. METHODS: Participants were selected through convenience sampling and consisted of 11 (2 female, 8 male, 1 prefer not to disclose; age = 28.1 ± 9.2 years; height = 176 ± 8.0 cm; mass = 73 ± 13.4 kg). Participants were all equipped with a COSMED K5 wearable metabolic system attached through a harness securely worn on their back. Outcome measures included Ventilation (VE [L/min]), Ventilatory Equivalent for Oxygen (VE/VO2), Ventilatory Equivalent for Carbon Dioxide (VE/VCO2), Tidal Volume (VT), and Respiratory Frequency (Rf). Alternating intervals of five minutes of play followed by five minutes of rest were consistent throughout. The order of using the DH or NDH was counterbalanced. Data were analyzed using a paired t-test with significance accepted at p £ 0.05. RESULTS: Significant differences were observed for VT (DH = 1.4 ± 0.3 vs. NDH = 1.3 ± 0.2, p = 0.05) and VE/VCO2 (DH = 30.9 ± 2.5 vs. NDH = 32 ± 2.9, p = 0.04). However, there were no significant differences found for VE in L/min (DH = 57.5 ± 9.7 ; NDH = 52.5 ± 11.6, p = 0.08), VE/VO2 (DH = 25.9 ± 2.1 vs. NDH = 26 ± 2.9, p = 0.43), or Rf (DH = 40.9 ± 4.1 vs. NDH = 41.3 ± 5.4, p = 0.36). CONCLUSION: The greater mean VE/VCO2 during NDH play compared to DH play suggests that the use of the NDH presents more difficulty performing pickleball-related tasks. Switching to the NDH is reflected in expiring more CO₂, indicating that players exert more effort when using their NDH. Although the respiration measures were similar in terms of exhalation, the use of DH caused a greater mean VT than NDH

Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions.

The SNF2 family of proteins includes representatives from a variety of species with roles in cellular processes such as transcriptional regulation (e.g. MOT1, SNF2 and BRM), maintenance of chromosome stability during mitosis (e.g. lodestar) and various aspects of processing of DNA damage, including nucleotide excision repair (e.g. RAD16 and ERCC6), recombinational pathways (e.g. RAD54) and post-replication daughter strand gap repair (e.g. RAD5). This family also includes many proteins with no known function. To better characterize this family of proteins we have used molecular phylogenetic techniques to infer evolutionary relationships among the family members. We have divided the SNF2 family into multiple subfamilies, each of which represents what we propose to be a functionally and evolutionarily distinct group. We have then used the subfamily structure to predict the functions of some of the uncharacterized proteins in the SNF2 family. We discuss possible implications of this evolutionary analysis on the general properties and evolution of the SNF2 family

Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae.

DNAs that contain specific yeast chromosomal sequences called ARSs transform Saccharomyces cerevisiae at high frequency and can replicate extrachromosomally as plasmids when introduced into S. cerevisiae by transformation. To determine the boundaries of the minimal sequences required for autonomous replication in S. cerevisiae, we have carried out in vitro mutagenesis of the first chromosomal ARS described, ARS1. Rather than identifying a distinct and continuous segment that mediates the ARS+ phenotype, we find three different functional domains within ARS1. We define domain A as the 11-base-pair (bp) sequence that is also found at most other ARS regions. It is necessary but not sufficient for high-frequency transformation. Domain B, which cannot mediate high-frequency transformation, or replicate by itself, is required for efficient, stable replication of plasmids containing domain A. Domain B, as we define it, is continuous with domain A in ARS1, but insertions of 4 bp between the two do not affect replication. The extent of domain B has an upper limit of 109 bp and a lower limit of 46 bp in size. There is no obvious sequence homology between domain B of ARS1 and any other ARS sequence. Finally, domain C is defined on the basis of our deletions as at least 200 bp flanking domain A on the opposite side from domain B and is also required for the stability of domain A in S. cerevisiae. The effect of deletions of domain C can be observed only in the absence of domain B, at least by the assays used in the current study, and the significance of this finding is discussed