2 research outputs found
HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery
The development of a useful methodology
for simple, scalable, and
transformative automation of oligosaccharide synthesis that easily
interfaces with existing methods is reported. The automated synthesis
can now be performed using accessible equipment where the reactants
and reagents are delivered by the pump or the autosampler and the
reactions can be monitored by the UV detector. The HPLC-based platform
for automation is easy to setup and adapt to different systems and
targets
Surface-Tethered Iterative Carbohydrate Synthesis: A Spacer Study
Comparative
study of Surface-Tethered Iterative Carbohydrate Synthesis
(STICS) using HPLC-assisted experimental setup clearly demonstrates
benefits of using longer spacer-anchoring systems. The use of mixed
self-assembled monolayers helps provide the required space for glycosylation
reaction around the immobilized glycosyl acceptor. Both extension
of the spacer length and using mixed self-assembled monolayers help
promote the reaction, and the beneficial effects may include moving
the glycosyl acceptor further out into solution and providing additional
conformational flexibility. It is possible that surface-immobilized
glycosyl acceptors with a longer spacer (C8–O–C8)-lipoic
acid have a higher tendency to mimic a solution-phase reaction environment
than acceptors with shorter spacers