Figure S1: This figure shows a boxplot of the OTU richnesses between all CTD casts collected on the East Scotia Ridge and the Southwest Indian Ridge. from Cutting through the smoke: diversity of free-living bacteria in deep-sea hydrothermal plumes

There are still notable gaps regarding the detailed distribution of microorganisms between and within insular habitats such as deep-sea hydrothermal vents. This study investigates the community composition of black smoker vent microorganisms in the Southern Hemisphere, and changes thereof along a spatial and chemical gradient ranging from the vent plume to surrounding waters. We sampled two hydrothermal vent fields, one at the South West Indian Ridge (SWIR), the other at the East Scotia Ridge (ESR). Samples were collected across vent fields at varying vertical distances from the origin of the plumes. The microbial data were sequenced on an Illumina MiSeq platform for the 16SrRNA gene. A substantial amount of vent-specific putative chemosynthetic microorganisms were found, particularly in samples from focused hydrothermal venting. Common vent-specific organisms from both vent fields were the genera <i>Arcobacter</i>, <i>Caminibacter</i> and <i>Sulfurimonas</i> from the Epsilonproteobacteria and the SUP05 group from the Gammaproteobacteria. There were no major differences in microbial composition between SWIR and ESR for focused plume samples. However, within the ESR the diffuse flow and focused samples differed significantly in microbial community composition and relative abundance. or Epsilonproteobacteria, we found evidence of niche-specificity to hydrothermal vent environments. This taxon decreased in abundance by three orders of magnitude from the vent orifice to background water. Epsilonproteobacteria distribution followed a distance–decay relationship as vent-effluents mixed with the surrounding seawater. This study demonstrates strong habitat affinity of vent microorganisms on a metre scale with distinct environmental selection

Figure S2:This barplot figure is of the sequence abundance of all the proteobacteria (A), and the SUP05 (B) from East Scotia Rdige hydrothermal vents, diffuse flow, and background samples. The y-axis is Eh, which represents a measure of how influenced the sample is by hydrothermal activity. The arrow represents the increase of hydrothermally influenced samples. from Cutting through the smoke: diversity of free-living bacteria in deep-sea hydrothermal plumes

There are still notable gaps regarding the detailed distribution of microorganisms between and within insular habitats such as deep-sea hydrothermal vents. This study investigates the community composition of black smoker vent microorganisms in the Southern Hemisphere, and changes thereof along a spatial and chemical gradient ranging from the vent plume to surrounding waters. We sampled two hydrothermal vent fields, one at the South West Indian Ridge (SWIR), the other at the East Scotia Ridge (ESR). Samples were collected across vent fields at varying vertical distances from the origin of the plumes. The microbial data were sequenced on an Illumina MiSeq platform for the 16SrRNA gene. A substantial amount of vent-specific putative chemosynthetic microorganisms were found, particularly in samples from focused hydrothermal venting. Common vent-specific organisms from both vent fields were the genera <i>Arcobacter</i>, <i>Caminibacter</i> and <i>Sulfurimonas</i> from the Epsilonproteobacteria and the SUP05 group from the Gammaproteobacteria. There were no major differences in microbial composition between SWIR and ESR for focused plume samples. However, within the ESR the diffuse flow and focused samples differed significantly in microbial community composition and relative abundance. or Epsilonproteobacteria, we found evidence of niche-specificity to hydrothermal vent environments. This taxon decreased in abundance by three orders of magnitude from the vent orifice to background water. Epsilonproteobacteria distribution followed a distance–decay relationship as vent-effluents mixed with the surrounding seawater. This study demonstrates strong habitat affinity of vent microorganisms on a metre scale with distinct environmental selection

The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications-1

Th mouse and human S100A4. A: Human S100A4 produced in-house using vector pGEX 3X with removal of GST-tag (previously shown in fig. 1E). B: Human his-tagged S100A4 produced in the lab of Prof. E. Lukanidin (Danish Cancer Society). C: Human S100A4 produced by Jena Biosciences. D: Mouse his-tagged S100A4 produced in-house using vector pQE30.<p><b>Copyright information:</b></p><p>Taken from "The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications"</p><p>http://www.biomedcentral.com/1471-2407/8/172</p><p>BMC Cancer 2008;8():172-172.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2443394.</p><p></p

The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications-2

All shown spots are identified in the SwissProt library as S100A4 with p < 0.05. A: cultured HCT116. B: Tumor biopsy from a colorectal cancer patient. C: Human recombinant S100A4 produced in-house. D: Representation of the S100A4 amino acid sequence with all tryptic peptides discovered on MALDI-TOF (boxes) and location of phosphorylations (P) and deamidations (D) predicted by computer algorithms.<p><b>Copyright information:</b></p><p>Taken from "The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications"</p><p>http://www.biomedcentral.com/1471-2407/8/172</p><p>BMC Cancer 2008;8():172-172.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2443394.</p><p></p

The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications-3

Y staining with MAb 22.3 against S100A4 and aligned according to the antibody light chain (not shown) detached during the immunoprecipitation procedure or added directly into the recombinant samples. A. Comparison of S100A4 in nuclear and cytoplasmic fractions isolated from cultured colorectal cancer cell line SW620 and extracellular S100A4 from conditioned growth medium by confluently growing SW620 cells. B. Comparison of S100A4 isolated from whole cell lysate of two cultured colorectal cancer cell lines with different TP53 status, SW620 (mut) and HCT116 (wt), and the OHS osteosarcoma cell line. C. Analysis of S100A4 isolated from biopsies collected from colorectal cancer patients diagnosed with different p53 status and Duke's stage. Two representative samples out of eight analyzed are shown. D. Endogenous S100A4 isolated from red blood cells (RBC) and mononuclear cells (MNC). E. Recombinant human S100A4 produced in-house using vector pGEX 3X (with removal of GST-tag).<p><b>Copyright information:</b></p><p>Taken from "The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications"</p><p>http://www.biomedcentral.com/1471-2407/8/172</p><p>BMC Cancer 2008;8():172-172.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2443394.</p><p></p

Supplementary Material The supplementary information contains four figures S1-S4. These figures depict the abundance, richness, and phylum level taxa between the seamounts analysed in this manuscript. In addition, we have a supplementary figure graphically showing the error output from the Multivariate Regression Trees run on the microbial and environmental data. from Microbe biogeography tracks water-masses in a dynamic oceanic frontal system

Dispersal limitation, not just environmental selection, plays an important role in microbial biogeography. The distance–decay relationship is thought to be weak in habitats where dispersal is high, such as in the pelagic environment, where ocean currents facilitate microbial dispersal. Most studies of microbial community composition to date have observed little geographical heterogeneity on a regional scale (100 km).We present a study of microbial communities across a dynamic frontal zone in the South West Indian Ocean and investigate the spatial structure of the microbes with respect to the different water masses separated by these fronts.We collected 153 samples of free-living microorganisms from five seamounts located along a gradient from subtropical to subantarctic waters and across three depth layers, (i) the sub-surface chlorophyll maximum (approx. 40 m), (ii) the bottom of the euphotic zone (approx. 200 m) and (iii) the benthic boundary layer (300–2000 m). Diversity and abundance of microbial operational taxonomic units (OTUs) was assessed by amplification and sequencing of the 16S rRNA gene on an Illumina MiSeq platform.Multivariate analyses showed that microbial communities were structured more strongly by depth than by latitude, with similar phyla occurring within each depth stratum across seamounts. The deep layer was homogeneous across the entire survey area, corresponding to the spread of Antarctic intermediate water. However, within both the sub-surface layer and the intermediate depth stratum there was evidence for OTU turnover across fronts. The microbiome of these layers appears to be divided into three distinct biological regimes corresponding to the subantarctic surface water, the convergence zone and subtropical. We show that microbial biogeography across depth and latitudinal gradients is linked to the water-masses the microbes persist in, resulting in regional patterns of microbial biogeography, that correspond to the regional scale physical oceanography

Read 1

Read 1 of Illumina Miseq run on 16S rDNA sequences

metadata

This metadata matches all the sequencing data from Read 1 and Read 2 in the same package

Read 2

Read 2 of Illumina Miseq run on 16S rDNA sequences