Activities of bone morphogenetic proteins in prolactin regulation by somatostatin analogs in rat pituitary GH3 cells

Involvement of the pituitary BMP system in the modulation of prolactin (PRL) secretion regulated by somatostatin analogs, including octreotide (OCT) and pasireotide (SOM230), and a dopamine agonist, bromocriptine (BRC), was examined in GH3 cells. GH3 cells are rat pituitary somato-lactotrope tumor cells that express somatostatin receptors (SSTRs) and BMP system molecules including BMP-4 and -6. Treatment with BMP-4 and -6 increased PRL and cAMP secretion by GH3 cells. The BMP-4 effects were neutralized by adding a BMP-binding protein Noggin. These findings suggest the activity of endogenous BMPs in augmenting PRL secretion by GH3 cells. BRC and SOM230 reduced PRL secretion, but OCT failed to reduce the PRL level. In GH3 cells activated by forskolin, BRC suppressed forskolin-induced PRL secretion with reduction in cAMP levels. OCT did not affect forskolin-induced PRL level, while SOM230 reduced PRL secretion and PRL mRNA expression induced by forskolin. BMP-4 treatment enhanced the reducing effect of SOM230 on forskolin-induced PRL level while BMP-4 did not affect the effects of OCT or BRC. Noggin treatment had no significant effect on the BRC actions reducing PRL levels by GH3 cells. However, in the presence of Noggin, OCT elicited an inhibitory effect on forskolin-induced PRL secretion and PRL mRNA expression, whereas the SOM230 effect on PRL reduction was in turn impaired. It was further found that BMP-4 and -6 suppressed SSTR-2 but increased SSTR-5 mRNA expression of GH3 cells. These findings indicate that Noggin rescues SSTR-2 but downregulates SSTR-5 by neutralizing endogenous BMP actions, leading to an increase in OCT sensitivity and a decrease in SOM230 sensitivity of GH3 cells. In addition, BMP signaling was facilitated in GH3 cells treated with forskolin. Collectively, these findings suggest that BMPs elicit differential actions in the regulation of PRL release dependent on cellular cAMP-PKA activity. BMPs may play a key role in the modulation of SSTR sensitivity of somato-lactotrope cells in an autocrine/paracrine manner

Enhanced Star Formation of Less Massive Galaxies in a Proto-Cluster at z=2.5

We investigate a correlation between star-formation rate (SFR) and stellar mass for Halpha emission line galaxies (HAEs) in one of the richest proto-clusters ever known at z~2.5, USS 1558-003 proto-cluster. This study is based on a 9.7-hour narrow-band imaging data with MOIRCS on the Subaru telescope. We are able to construct a sample, in combination with additional H-band data taken with WFC3 on Hubble Space Telescope (HST), of 100 HAEs reaching the dust-corrected SFRs down to 3 Msun/yr and the stellar masses down to $10^{8.0}$ Msun. We find that while the star-forming galaxies with >$10^{9.3}$ Msun are located on the universal SFR-mass main sequence irrespective of the environment, less massive star-forming galaxies with <$10^{9.3}$ Msun show a significant upward scatter from the main sequence in this proto-cluster. This suggests that some less massive galaxies are in a starburst phase, although we do not know yet if this is due to environmental effects.Comment: 5 pages, 3 figures, 1 table, accepted for publication in the ApJ Letter

Research Notes : United States : New independent mutation: msl (Ames 2)

Five different populations have been recognized as a source of msl alleles. Genetics studies of male-sterile, female-fertile mutations conducted by Palmer et al. (1978) showed that msl (North Carolina) (T260), msl (Urbana) (T266), msl (Tonica) (T267), and msl (Ames) (T268) are independent mutations at the msl locus. Yee and Jian (1983) reported another mutation at the msl locus, designated Shennong Male-Sterile Soybean L-78-387

The Bright Side and Dark Side of Workplace Social Capital: Opposing Effects of Gender on Overweight among Japanese Employees

Background: A growing number of studies have sought to examine the health associations of workplace social capital; however, evidence of associations with overweight is sparse. We examined the association between individual perceptions of workplace social capital and overweight among Japanese male and female employees. Methodology/Principal Findings We conducted a cross-sectional survey among full-time employees at a company in Osaka prefecture in February 2012. We used an 8-item measure to assess overall and sub-dimensions of workplace social capital, divided into tertiles. Of 1050 employees, 849 responded, and 750 (624 men and 126 women) could be linked to annual health check-up data in the analysis. Binomial logistic regression models were used to calculate odds ratios and 95% confidence intervals for overweight (body mass index: ≥25 kg/m2, calculated from measured weight and height) separately for men and women. The prevalence of overweight was 24.5% among men and 14.3% among women. Among men, low levels of bonding and linking social capital in the workplace were associated with a nearly 2-fold risk of overweight compared to high corresponding dimensions of social capital when adjusted for age, sleep hours, physiological distress, and lifestyle. In contrast, among women we found lower overall and linking social capital to be associated with lower odds for overweight even after covariate adjustment. Subsequently, we used multinomial logistic regression analyses to assess the relationships between a 1 standard deviation (SD) decrease in mean social capital and odds of underweight/overweight relative to normal weight. Among men, a 1-SD decrease in overall, bonding, and linking social capital was significantly associated with higher odds of overweight, but not with underweight. Among women, no significant associations were found for either overweight or underweight. Conclusions/Significance: We found opposite gender relationships between perceived low linking workplace social capital and overweight among Japanese employees

ALMA Millimeter/Submillimeter Sources among Spitzer SMUVS Galaxies at z &gt; 2 in the COSMOS Field

Submillimeter observations reveal the star formation activity obscured by dust in the young Universe. It still remains unclear how galaxies detected at submillimeter wavelengths are related to ultraviolet/optical-selected galaxies in terms of their observed quantities, physical properties, and evolutionary stages. Deep near- and mid-infrared observational data are crucial to characterize the stellar properties of galaxies detected with submillimeter emission. In this study, we make use of a galaxy catalog from the Spitzer Matching survey of the UltraVISTA ultra-deep Stripes. By crossmatching with a submillimeter source catalog constructed with archival data of the Atacama Large Millimeter/submillimeter Array (ALMA), we search for galaxies at z &gt; 2 with a submillimeter detection in our galaxy catalog. We find that the ALMA-detected galaxies at z &gt; 2 are systematically massive and have redder K s -[4.5] colors than the nondetected galaxies. The redder colors are consistent with the larger dust reddening values of the ALMA-detected galaxies obtained from spectral energy distribution (SED) fitting. We also find that the ALMA-detected galaxies tend to have brighter 4.5 μm magnitudes. This may suggest that they tend to have smaller mass-to-light ratios and thus to be younger than star-forming galaxies fainter at submillimeter wavelengths with similar stellar masses. We identify starburst galaxies with high specific star formation rates among both ALMA-detected and nondetected SMUVS sources. Irrespective of their brightness at submillimeter wavelengths, these populations have similar dust reddening values, which may suggest a variety of dust SED shapes among the starburst galaxies at z &gt; 2.</p

Perceptual Continuity and Naturalness of Expressive Strength in Singing Voices Based on Speech Morphing

This paper experimentally shows the importance of perceptual continuity of the expressive strength in vocal timbre for natural change in vocal expression. In order to synthesize various and continuous expressive strengths with vocal timbre, we investigated gradually changing expressions by applying the STRAIGHT speech morphing algorithm to singing voices. Here, a singing voice without expression is used as the base of morphing, and singing voices with three different expressions are used as the target. Through statistical analyses of perceptual evaluations, we confirmed that the proposed morphing algorithm provides perceptual continuity of vocal timbre. Our results showed the following: (i) gradual strengths in absolute evaluations, and (ii) a perceptually linear strength provided by the calculation of corrected intervals of the morph ratio by the inverse (reciprocal) function of an equation that approximates the perceptual strength. Finally, we concluded that applying continuity was highly effective for achieving perceptual naturalness, judging from the results showing that (iii) our gradual transformation method can perform well for perceived naturalness