An improved method for the highly specific detection of transcription start sites

遺伝子の転写開始点の検出法TSS-seq2を開発 --メッセンジャーRNAの5’末端を高い特異性で検出--. 京都大学プレスリリース. 2023-11-28.Precise detection of the transcriptional start site (TSS) is a key for characterizing transcriptional regulation of genes and for annotation of newly sequenced genomes. Here, we describe the development of an improved method, designated ‘TSS-seq2.’ This method is an iterative improvement of TSS-seq, a previously published enzymatic cap-structure conversion method to detect TSSs in base sequences. By modifying the original procedure, including by introducing split ligation at the key cap-selection step, the yield and the accuracy of the reaction has been substantially improved. For example, TSS-seq2 can be conducted using as little as 5 ng of total RNA with an overall accuracy of 96%; this yield a less-biased and more precise detection of TSS. We then applied TSS-seq2 for TSS analysis of four plant species that had not yet been analyzed by any previous TSS method

Attitude Control Simulator for the Small Satellite and Its Validation by On-orbit Data of QSAT-EOS

The role of the microsatellites has become significant due to the progress of performance and miniaturization of the electronics devices. For the purpose of earth observation the constellation flight of the microsatellites has made it possible to obtain the continuous data of the designated location and it is expected that the data be utilized in wide variety of the filed such as science, agriculture, industries, and climate monitoring. As the microsatellites take the LEO, the time of observation on the designated location is limited. Therefore the agile and precise pointing performance is required so as the attitude control accuracies. This paper presents the development of the attitude control simulator for the microsatellite for the use of design of the attitude control subsystem. Quaternion feedback control is proved to be effective and stable. Then the on-orbit data of the QSAT-EOS (Kyushu Satellite for Earth Observation System Demonstration) is analyzed to confirm the effectiveness of the quaternion feedback control. QSAT-EOS was launched in 2014 and now is in the deorbit phase after two year operation. Results of the on-orbit data analysis have suggested some necessities of improvement of the simulator and control algorithm which are the future subject

Development of Total Orbital Real-Time Attitude Control Simulator for Small Satellites

This paper presents the overview of the mathematical simulator TORA-Sim (Total Orbital Real-time Attitude control Simulator) developed by Kyushu University and Mitsubishi Precision Co.,Ltd. used for the satellite attitude control and the simulation results for the typical small satellite application. The results have demonstrated the validity of this simulator in use for the study of the attitude control law. This simulator was used for the development of onboard software for the attitude control of the Kyushu University’s small satellite “QSAT-EOS” launched last autumn