Focus Groups in Ethnography of Communication: Expanding Topics of Inquiry Beyond Participant Observation

Historically, ethnography of communication has viewed participant observation as the central and necessary methodological point of departure for any ethnographic endeavor. However, as this article illustrates, this becomes problematic when particular topics of inquiry do not provide ample opportunities for observation. After struggles to participant observe conversations on women\u27s marital naming practices, I was able to research this topic and produce participant observation-like understandings after I incorporated focus groups into my methods. Based upon these experiences and study, this article urges ethnography of communication scholars to step outside their traditional methodological practices, when necessary, and integrate the focus group method into their research protocols

Gender Role and Feminism Revisited: A Follow-Up Study

In this follow-up to our earlier study (Toller, Suter, & Trautman, Gender role identity and attitudes towards feminism, Sex Roles, 51, 85–90, 2004) we examine the interrelationships among gender role, support for feminism, and willingness to self-label as feminist. Ten percent of college students previously surveyed participated in qualitative interviews, which elicited characterizations of feminists, whether students self-identified as feminist, suggestions for garnering support for feminism, and for interpretation of the initial study’s findings. Students were asked to speculate why we found that highly masculine men and highly feminine women were neither likely to self-identify as feminist nor to support the feminist movement and why more feminine men and more masculine women were found to be more willing to self-label as feminist and more likely to support the feminist movement

Gender Role Identity and Attitudes Toward Feminism

In this study we examined relationships among gender role identity, support for feminism, nontraditional gender roles, and willingness to consider oneself a feminist in a sample of college students (N D 301). For female participants, we found positive relationships among higher masculinity on the PAQ (Personal Attributes Questionnaire), nontraditional attitudes toward gender roles, and the combined SRAI (Sex Role Attitudinal Inventory). A negative correlation was also found between lower scores on the PAQ masculinity–femininity index and the combined SRAI in women. For male participants, we found positive relationships among high femininity on the SIS (Sexual Identity Scale), willingness to consider oneself a feminist, positive attitudes toward the women’s movement, and the combined SRAI. We also found a negative relationship between high masculinity on the PAQ and willingness to consider oneself a feminist in men. The implications of these findings for the feminist movement are discussed

Particle Association of Enterococcus and Total Bacteria in the Lower Hudson River Estuary, USA

Bacterial particle association has important consequences for water-quality monitoring and modeling. Parti-cle association can change vertical and horizontal transport of bacterial cells, as well as patterns of persis-tence and production. In this study, the abundance and particle association of total bacteria and the fe-cal-indicator, Enterococcus, were quantified between June and October 2008 in the lower Hudson River Es-tuary (HRE). Twelve sites were sampled, including mid-channel, near shore, and tributary habitats, plus a sewage outfall. Total bacterial cell counts averaged 9.2 × 109 ± 6.4 × 109 cell·l–1 (1 standard deviation), com-parable to previous sampling in the HRE. Unlike earlier studies, bacterial abundance did not change consis-tently along the north/south estuarine salinity gradient. Enterococcus concentrations were highly variable, but mid-channel stations had significantly lower values than other habitat categories. Counts of total bacteria and Enterococci were both correlated with turbidity, which was also significantly lower at mid-channel sta-tions. A larger fraction of Enterococci were associated with particles (52.9% ± 20.9%, 1 standard deviation) than in the pool of total bacteria (23.8% ± 15.0%). This high frequency of particle association, relative to total bacteria, could cause Enterococci to be preferentially retained near input sources because of enhanced deposition to bottom sediments, where they would be available for later resuspension. In turn, retention and resuspension in nearshore environments may explain the observed cross-channel variability of turbidity and Enterococci. Assessments and predictive models of estuarine water quality may be improved by incorporat-ing cross-channel variability and the effects of particle association on key indicators

A Tale of Two Mommies: (Re)Storying Family of Origin Narratives

This study examined co-mother family of origin stories. Origin stories, representing the formation of a family, are culturally understood within a master narrative of heterosexual love and biological childbearing. Beginnings of co-mother families rupture this dominant, gendered, boy-meets-girl script. Investigating whether or not co-mother stories reify the normative master narrative or if instead their narrations resist and/or possibly transform conventional understandings, analysis identified three co-mother origin story themes: Becoming a Family (1) as Normal, (2) as Negotiation, and (3) as Normalization. Themes differ in terms of depiction of co-mother family formation as congruent with current norms, as something that needs to be made to seem normal (i.e., in need of normalization), or as something between normal and normalization—to be negotiated internally within the couple. Study results are discussed within a broader framework of family coming-together stories

A Tale of Two Mommies: (Re)Storying Family of Origin Narratives

This study examined co-mother family of origin stories. Origin stories, representing the formation of a family, are culturally understood within a master narrative of heterosexual love and biological childbearing. Beginnings of co-mother families rupture this dominant, gendered, boy-meets-girl script. Investigating whether or not co-mother stories reify the normative master narrative or if instead their narrations resist and/or possibly transform conventional understandings, analysis identified three co-mother origin story themes: Becoming a Family (1) as Normal, (2) as Negotiation, and (3) as Normalization. Themes differ in terms of depiction of co-mother family formation as congruent with current norms, as something that needs to be made to seem normal (i.e., in need of normalization), or as something between normal and normalization—to be negotiated internally within the couple. Study results are discussed within a broader framework of family coming-together stories

Salty sensors, fresh ideas: The use of molecular and imaging sensors in understanding plankton dynamics across marine and freshwater ecosystems

Understanding plankton dynamics in marine ecosystems has been advanced using in situ molecular and imaging instrumentation. A range of research objectives have been addressed through high‐resolution autonomous sampling, from food web characterization to harmful algal bloom dynamics. When used together, molecular and imaging sensors can cover the full‐size range, genetic identity, and life stages of plankton. Here, we briefly review a selection of in situ instrumentation developed for the collection of molecular and imaging information on plankton communities in marine ecosystems. In addition, we interviewed a selection of instrumentation developers to determine if the transfer of sensor technology from marine to freshwater ecosystems is feasible and to describe the process of creating in situ sensors. Finally, we discuss the status of in situ molecular and imaging sensors in freshwater ecosystems and how some of the reviewed sensors could be used to address basic and applied research questions

Eukaryotic parasites are integral to a productive microbial food web in oxygen-depleted waters

© The Author(s), 2022]. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Suter, E. A., Pachiadaki, M., Taylor, G. T., & Edgcomb, V. P. Eukaryotic parasites are integral to a productive microbial food web in oxygen-depleted waters. Frontiers in Microbiology, 12, (2022): 764605, https://doi.org/10.3389/fmicb.2021.764605.Oxygen-depleted water columns (ODWCs) host a diverse community of eukaryotic protists that change dramatically in composition over the oxic-anoxic gradient. In the permanently anoxic Cariaco Basin, peaks in eukaryotic diversity occurred in layers where dark microbial activity (chemoautotrophy and heterotrophy) were highest, suggesting a link between prokaryotic activity and trophic associations with protists. Using 18S rRNA gene sequencing, parasites and especially the obligate parasitic clade, Syndiniales, appear to be particularly abundant, suggesting parasitism is an important, but overlooked interaction in ODWC food webs. Syndiniales were also associated with certain prokaryotic groups that are often found in ODWCs, including Marinimicrobia and Marine Group II archaea, evocative of feedbacks between parasitic infection events, release of organic matter, and prokaryotic assimilative activity. In a network analysis that included all three domains of life, bacterial and archaeal taxa were putative bottleneck and hub species, while a large proportion of edges were connected to eukaryotic nodes. Inclusion of parasites resulted in a more complex network with longer path lengths between members. Together, these results suggest that protists, and especially protistan parasites, play an important role in maintaining microbial food web complexity, particularly in ODWCs, where protist diversity and microbial productivity are high, but energy resources are limited relative to euphotic waters.This work was supported by the National Science Foundation (NSF) grants (OCE-1336082 to VE and OCE-1335436 and OCE-1259110 to GT). The Cyverse infrastructure and resources are supported by the NSF under Award Numbers DBI-0735191, DBI-1265383, and DBI-1743442 (www.cyverse.org). Support was also provided by the Faculty Scholarship and Academic Advancement Committee at Molloy College

Hidden in plain sight: The importance of cryptic interactions in marine plankton

Here, we present a range of interactions, which we term “cryptic interactions.” These are interactions that occur throughout the marine planktonic foodweb but are currently largely overlooked by established methods, which mean large-scale data collection for these interactions is limited. Despite this, current evidence suggests some of these interactions may have perceptible impacts on foodweb dynamics and model results. Incorporation of cryptic interactions into models is especially important for those interactions involving the transport of nutrients or energy. Our aim is to highlight a range of cryptic interactions across the plankton foodweb, where they exist, and models that have taken steps to incorporate these interactions. Additionally, it is discussed where additional research and effort is required to continue advancing our understanding of these cryptic interactions. We call for more collaboration between ecologists and modelers in order to incorporate cryptic interactions into biogeochemical and foodweb models

Diverse secondary metabolites are expressed in particle-associated and free-living microorganisms of the permanently anoxic Cariaco Basin

Secondary metabolites play essential roles in ecological interactions and nutrient acquisition, and are of interest for their potential uses in medicine and biotechnology. Genome mining for biosynthetic gene clusters (BGCs) can be used for the discovery of new compounds. Here, we use metagenomics and metatranscriptomics to analyze BGCs in free-living and particle-associated microbial communities through the stratified water column of the Cariaco Basin, Venezuela. We recovered 565 bacterial and archaeal metagenome-assembled genomes (MAGs) and identified 1154 diverse BGCs. We show that differences in water redox potential and microbial lifestyle (particle-associated vs. free-living) are associated with variations in the predicted composition and production of secondary metabolites. Our results indicate that microbes, including understudied clades such as Planctomycetota, potentially produce a wide range of secondary metabolites in these anoxic/euxinic waters