A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain-1 gene

The Italian-native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into the wild for angling. A PCR-restriction fragment length polymorphism (RFLP) assay of the diagnostic lactate dehydrogenase chain-1 (LDH-C1) gene sequences has been routinely applied to distinguish exotic from native brown trout lineages and detect Atlantic introgression signals in the Mediterranean wild populations. Here, we used dermal swab DNA obtained from 28 wild trout to improve laboratory procedures to genetically characterise trout samples at the LDH-C1gene through (1) a capillary electrophoresis analysis of the RFLP fragments and (2) the optimisation of a diagnostic single nucleotide polymorphism analysable through mini-sequencing approaches. The developed methods were fully consistent with those obtained through the traditional approach, but their analytical process is almost entirely automated and digitalised, thus improving result readability and accuracy in the detection of alien introgressed traces in wild Mediterranean brown trout populations

Mindfulness Meditation Leads To Increased Dispositional Mindfulness And Interoceptive Awareness Linked To A Reduced Dissociative Tendency

Dissociation is an involuntary defensive mechanism to protect oneself by avoiding unbearable internal conflicts or overwhelming emotions. Cultivating mindful awareness could allow the development of voluntary processes that can offer part of the self-protective function of dissociation while favoring internal integration processes. The aim of the present study was to explore the effects of a 7-week Mindfulness Oriented Meditation (MOM) training on healthy individuals' self-reported dissociative experience, mindfulness skills and interoceptive awareness. After the training, in comparison to a waiting-list control group (N\ua0=\ua0102), the MOM group (N\ua0= 110) showed reduced dissociative tendencies (p < .05), increased dispositional mindfulness (p <\ua0.001) and increased interoceptive awareness (in the aspects of not-worrying, self-regulation and body listening; p <\ua0.001). Moreover, correlational evidence showed that the more MOM participants increased in mindfulness skills after the training the more they reported increased interoceptive awareness and decreased dissociative functioning (p <\ua0.05). Mindfulness skills also improved with more home meditation practice executed by MOM participants (p <\ua0.05). These findings were attributed to a possible role of mindfulness meditation in enabling the development of volitional processes that afford psychological safety and integration, in contrast with the involuntary nature of dissociation

The management of acute venous thromboembolism in clinical practice - study rationale and protocol of the European PREFER in VTE Registry

Background: Venous thromboembolism (VTE) is a major health problem, with over one million events every year in Europe. However, there is a paucity of data on the current management in real life, including factors influencing treatment pathways, patient satisfaction, quality of life (QoL), and utilization of health care resources and the corresponding costs. The PREFER in VTE registry has been designed to address this and to understand medical care and needs as well as potential gaps for improvement. Methods/design: The PREFER in VTE registry was a prospective, observational, multicenter study conducted in seven European countries including Austria, France Germany, Italy, Spain, Switzerland, and the UK to assess the characteristics and the management of patients with VTE, the use of health care resources, and to provide data to estimate the costs for 12 months treatment following a first-time and/or recurrent VTE diagnosed in hospitals or specialized or primary care centers. In addition, existing anticoagulant treatment patterns, patient pathways, clinical outcomes, treatment satisfaction, and health related QoL were documented. The centers were chosen to reflect the care environment in which patients with VTE are managed in each of the participating countries. Patients were eligible to be enrolled into the registry if they were at least 18 years old, had a symptomatic, objectively confirmed first time or recurrent acute VTE defined as either distal or proximal deep vein thrombosis, pulmonary embolism or both. After the baseline visit at the time of the acute VTE event, further follow-up documentations occurred at 1, 3, 6 and 12 months. Follow-up data was collected by either routinely scheduled visits or by telephone calls. Results: Overall, 381 centers participated, which enrolled 3,545 patients during an observational period of 1 year. Conclusion: The PREFER in VTE registry will provide valuable insights into the characteristics of patients with VTE and their acute and mid-term management, as well as into drug utilization and the use of health care resources in acute first-time and/or recurrent VTE across Europe in clinical practice. Trial registration: Registered in DRKS register, ID number: DRKS0000479