Tropidosteptes forestierae (Hemiptera: Heteroptera: Miridae: Mirinae): a new species of Plant Bug injuring ornamental Florida Swampprivet, Forestiera segregata (Oleaceae), in South Florida

The mirine plant bug Tropidosteptes forestierae, new species (Hemiptera: Miridae) is described from Collier County, Florida, where it was found causing serious injury to an extensive ornamental hedge of Florida swampprivet, Forestiera segregata (Jacq.) Krug and Urb. (Oleaceae). Adult male and female, fifth instar, and egg are described. Color images of the adults, nymph, egg, and injury; scanning photomicrographs of selected adult structures; and illustrations of male genitalia are provided. A key to help distinguish the 16 species of Tropidosteptes known to occur in the southeastern United States is given

Cemented mounds and hydrothermal sediments on the detachment surface at Kane Megamullion : a new manifestation of hydrothermal venting

Author Posting. © American Geophysical Union, 2013. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry, Geophysics, Geosystems 14 (2013): 3352–3378, doi:10.1002/ggge.20186.Long-lived detachment faults are now known to be important in tectonic evolution of slow-spreading mid-ocean ridges, and there is increasing evidence that fluid flow plays a critical role in development of detachment systems. Here we document a new manifestation of low-temperature hydrothermal venting associated with the detachment fault that formed Kane Megamullion ∼3.3–2.1 m.y. ago in the western rift-valley wall of the Mid-Atlantic Ridge. Hydrothermal effects on the detachment surface include (1) cemented mounds of igneous rock and chalk debris containing hydrothermal Mn oxides and Fe oxyhydroxides, and (2) layered deposits of similar Fe-Mn minerals ± interbedded chalks. Mounds are roughly conical, ∼1–10 m high, and contain primarily basalts with lesser gabbro, serpentinite, and polymict breccia. The layered Fe-Mn-rich sediments are flat-bedded to contorted and locally are buckled into low-relief linear or polygonal ridges. We propose that the mounds formed where hydrothermal fluids discharged through the detachment hanging wall near the active fault trace. Hydrothermal precipitates cemented hanging-wall debris and welded it to the footwall, and this debris persisted as mounds as the footwall was exhumed and surrounding unconsolidated material sloughed off the sloping detachment surface. Some of the layered Fe-Mn-rich deposits may have precipitated from fluids discharging from the hanging-wall vents, but they also precipitated from low-temperature fluids venting from the exposed footwall through overlying chalks. Observed natural disturbance and abnormally thin hydrogenous Fe-Mn crusts on some contorted, hydrothermal Fe-Mn-rich chalks on ∼2.7 Ma crust suggest diffuse venting that is geologically recent. Results of this study imply that there are significant fluid pathways through all parts of detachment systems and that low-temperature venting through fractured detachment footwalls may continue for several million years off-axis.NSF grant 0118445 supported data acquisition and processing for Knorr Cruise 180- 2. The Deep Ocean Exploration Institute at Woods Hole Oceanographic Institution supported research and analytical costs for this study.2014-03-0

Ceramic Microchips for Capillary Electrophoresis-electrochemistry

A capillary electrophoresis–electrochemistry chip constructed from low-temperature co-fired ceramic (LTCC) tape is presented. This is the first report of such a chip constructed in this manner using these materials. Electroosmotic flow at pH 7 is demonstrated by the migration of a neutral marker, catechol. The separation and detection of two phenolic compounds are presented

Evaluation of site-specific methylation of the CMV promoter and its role in CHO cell productivity of a recombinant monoclonal antibody

We previously demonstrated that increased monoclonal antibody productivity in dihydrofolate reductase (DHFR)-amplified CHO cells correlates with phosphorylated transcription factor-cytomegalovirus (CMV) promoter interactions. In this article, we extend the characterization to include CMV promoter methylation and its influence on NFκB and CREB1 transcription factor binding to the CMV promoter in two families of DHFR-amplified CHO cell lines. CMV promoter methylation was determined using bisulfite sequencing. To overcome Sanger-sequencing limitations due to high CG bias and multiple transgenes copies, pyrosequencing was used to determine the frequency of methylated cytosines in regions proximal to and containing the NFκB and CREB1 transcription-factor consensus binding sites. Chromatin immunoprecipitation was performed to interrogate transcription factor-DNA interactions. Antibodies to CREB1 and NFκB were used to immunoprecipitate formaldehyde-crosslinked protein-DNA fractions, followed by reverse transcription quantitative real-time polymerase chain reaction to quantitate the number of copies of CMV-promoter DNA bound to the various transcription factors. The relative unmethylated fraction at the CREB1 and NFκB consensus binding sites determined by pyrosequencing was correlated with transcription factor binding as determined by chromatin immunoprecipitation. Azacytidine treatment reduced methylation in all treated samples, though not at all methylation sites, while increasing transcription. Distinct promoter methylation patterns arise upon clonal selection in different families of cell lines. In both cell line families, increased methylation was observed upon amplification. In one family, the NFκB binding-site methylation was accompanied by increased CREB1 interaction with the promoter. In the other cell line family, lower methylation frequency at the NFκB consensus binding site was accompanied by more NFκB recruitment to the promoter region

Integrated management of invasive cattails (Typha spp.) for wetland habitat and biofuel in the Northern Great Plains of the United States and Canada: A review

On many public lands in the Great Plains region of the USA and Canada, cattail (Typha spp.) growth has far exceeded the 50:50 distribution recommended for optimum wetland wildlife habitat. Excessive cattail growth is the primary concern of wetland managers and its integrated management is reviewed here. The coverage of this mostly hybrid cattail (T. latifolia × T. angustifolia) is often over 90 % and if partially removed for habitat enhancement represents a substantial biomass resource in sites such as conservation wetlands, water retention basins and roadside drainage ditches. Available biomass is estimated to be 3,000 kg/ha assuming a 50 % harvest rate. Cattail control using mowing, herbicides, and burning is expensive, therefore if harvest logistics can be improved along with developing biomass markets, harvest management would become much more viable. Energy values of cattails are comparable to wood pellets at 20 MJ/kg. Cattails can be simultaneously managed for wetland wildlife, harvested for biofuel, serve as a partial substitute for coal, generate carbon credits, and remove phosphorus from the watershed. Cattails extract nitrogen and phosphorous from runoff water that enters rivers and lakes that could be used for agricultural fertiliser while reducing eutrophication. Additionally, rural economies could be boosted by harvesting a renewable energy resource, especially in areas with little fossil fuels or unsustainable biomass practices