Hemispherectomy with corpus callosotomy in pediatric Lennox Gastaut Syndrome associated encephalomalacia cyst: The first case in Indonesia

Lennox-Gastaut syndrome (LGS) is a form of severe epileptic encephalopathy in children. LGS with encephalomalacia cysts is rare in children. We report a six-year-old mentally retarded boy who was referred for an intractable seizure. Seizures were tonic, atonic, and dialeptic in frequency. EEG showed generalized SSW discharges of 1.5-2 Hz, polyspikes, and burst suppression typical for LGS. Head MRI showed an encephalomalacia cyst in the right subcortical temporoparietal lobes with hemiatrophy in the right cerebral hemisphere. He was already treated with three antiepileptic drugs, but the seizures persisted. The patient was then performed right hemispherectomy and corpus callosotomy. It resulted in a good response. A combination of hemispherectomy and corpus callosotomy could be promising in this form of epilepsy disease. Seizure reduction was achieved and showed cognitive improvement and hemiparesis

Ekstraksi Dna Collocalia Fuchiphaga Dengan Metode Phenol Chloroform Extraction Dari Berbagai Material Sumber Genetik

DNA extraction is the first important step in molecular study. The aim of this research was to compare DNA extraction protocols, PCE and extraction kit for each genetic material sources (blood and feathers). For PCE method, we used three different extraction buffer. This study suggested that PCE method was more efficient than the extraction kit method. Meanwhile, extraction buffer 2 was more efficient for extracting feather DNA, while extraction buffer 3 was more efficient for extracting blood DNA. Wing feather was a favorable sample as genetic source for DNA extraction