Genetic diversity among three Indian populations of black tiger shrimp (Penaeus monodon Fabricious, 1798) using microsatellite DNA markers

Genetic variation is an essential factor while selecting superior stocks for breeding programs. Eight microsatellite loci (TUZX pm2.27, TUZX pm2.28, TUZX pm2.36, TUZX pm2.69, TUZX pm4.84, TUZX pm4.87, TUZX pm6.1 and TUZX pm4.9) were used for molecular characterisation of Penaeus monodon collected from three different locations in India, viz., Kakinada and Chennai on the east coast and Mumbai on the west coast and all the loci were found to be highly polymorphic. The number of alleles and genotypes across the loci ranged from 5 to 23 and 42 to 98, respectively. The observed heterozygosity among the populations across the loci ranged from 0 to 0.1678. The overall FST value was found to be 0.021 (<0.05), which implies a low degree of population differentiation in the species. On the basis of pair-wise allelic differentiation and UPGMA dendrogram, the investigated samples from three locations were allocated in to two distinct populations composed of east coast and west coast populations

Growth promoting activity of Pangasianodon hypophthalmus recombinant growth hormone expressed in Escherichia coli

Recombinant growth hormone of Pangasianodon hypophthalmus (rPhGH) was efficiently expressed in Escherichia coli BL 21 (DE3) cells. The expression vector pET-32a(+) was used to clone and express a 550 bp long cDNA fragment, which encodes the mature region of growth hormone. The rPhGH was expressed as a 6X HIS-tag fusion protein in E. coli upon induction by Isopropyl b-D-thiogalactoside, and formed insoluble inclusion bodies in the host cells. SDS-PAGE analysis indicated that the molecular weight of the fusion protein was about 23 kDa, which is comparable to the theoretical value of the mature growth hormone of the fish. The expressed protein was recovered by solubilising the inclusion bodies under denaturing conditions with urea and then the denatured proteins were refolded and purified on Ni-NTA column. The purified recombinant protein was confirmed by Western blot analysis using anti-His antibodies. Total yield of the refolded and purified protein was 20 mg l-1 of LB medium. Biological activity of the purified recombinant protein was determined in in vivo bioassay by its ability to promote growth in rohu (Labeo rohita) fingerlings, injected with three different concentrations of the hormone. A significant increase in growth was observed in rohu fingerlings administered with rPhGH at a dosage of 1.0 mg g body weight -1

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Not AvailableGenetic variation is an essential factor while selecting superior stocks for breeding programs. Eight microsatellite loci (TUZX pm2.27, TUZX pm2.28, TUZX pm2.36, TUZX pm2.69, TUZX pm4.84, TUZX pm4.87, TUZX pm6.1 and TUZX pm4.9) were used for molecular characterisation of Penaeus monodon collected from three different locations in India, viz., Kakinada and Chennai on the east coast and Mumbai on the west coast and all the loci were found to be highly polymorphic. The number of alleles and genotypes across the loci ranged from 5 to 23 and 42 to 98, respectively. The observed heterozygosity among the populations across the loci ranged from 0 to 0.1678. The overall FST value was found to be 0.021 (<0.05), which implies a low degree of population differentiation in the species. On the basis of pair-wise allelic differentiation and UPGMA dendrogram, the investigated samples from three locations were allocated in to two distinct populations composed of east coast and west coast populations.Not Availabl

Not Available

Not AvailableGenetic variation is an essential factor while selecting superior stocks for breeding programs. Eight microsatellite loci (TUZX pm2.27, TUZX pm2.28, TUZX pm2.36, TUZX pm2.69, TUZX pm4.84, TUZX pm4.87, TUZX pm6.1 and TUZX pm4.9) were used for molecular characterisation of Penaeus monodon collected from three different locations in India, viz., Kakinada and Chennai on the east coast and Mumbai on the west coast and all the loci were found to be highly polymorphic. The number of alleles and genotypes across the loci ranged from 5 to 23 and 42 to 98, respectively. The observed heterozygosity among the populations across the loci ranged from 0 to 0.1678. The overall FST value was found to be 0.021 .Indian Council of Agricultural Research (ICAR), New Delhi, Indi

Microsatellite DNA Analysis of Giant Freshwater Prawn (Macrobrachium rosenbergii) from India

Giant freshwater prawn (Macrobrachium rosenbergii), a commercially important crustacean species, is widely distributed across the Indo-Pacific region. Genetic diversity of this species from five different rivers (Krishna, Mahanadi, Hooghly, Narmada and Kalu) of India was investigated using 5 polymorphic microsatellite loci. The number of alleles across loci varied from 4 to 9. The mean expected and observed heterozygosity at all loci was 0.8359 and 0.5747 respectively. Most of the loci deviated significantly from Hardy-Weinberg expectations across all the populations. Pairwise FST estimates (0.0420 to 0.0841) revealed a significant genetic structure among M. rosenbergii populations of Indian rivers. The highest (0.5140) genetic distance was observed between Krishna and Kalu populations. All five wild populations exhibited significant variation across all five microsatellite loci. The results revealed in the study will be useful for breeding programs and conservation management of this species