HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand

Phellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1 - 10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099 - 0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms

การวิเคราะห์ปริมาณสารสำคัญและฤทธิ์ต้านอนุมูลอิสระของสารสกัดใบกะเปียดเครือ

วารสารวิชาการและวิจัย มทร.พระนคร, ปีที่ 15, ฉบับที่ 2 (ก.ค.-ธ.ค. 2564), หน้า 118-129Premna trichostoma Miq. (Lamiaceae) mainly distribute in Asia including Thailand. Folk healers use this plant to treat fever as a juice, splash, and bathe. The aims of this study are to evaluate the quantity of active constituents and antioxidant activity of P. trichostoma leaf extract. Total phenolic compounds were determined using Folin-Ciocalteu method. Total flavonoid contents were determined by Aluminum chloride method. The amount of apigenin and kaempferol contents were analyzed by high performance liquid chromatography (HPLC). In addition, the antioxidant activity was evaluated by DPPH and ABTS assay. The results showed that total phenolic contents, total flavonoid contents, and the amount of apigenin and kaempferol of P. trichostoma leaf extract was 46.45±15.30 mg GAE/g extract, 25.48±2.02 mg CE/g extract, 11.16±0.04 and 4.54±0.08 µg/g extract, respectively. The IC50 values from the DPPH and ABTS assay was 117.50±1.22 and 36.14±1.53 µg/ml, respectively. This study shows the antioxidant properties and active constituents of P. trichostoma leaf extract which indicates the potentials for further development of this plantRajamangala University of Technology Phra Nakho