A study on the differentiation of filamentous cyanobacterial isolates using DNA fingerprinting approach

337-345The present study investigates the implication of DNA fingerprinting in discrimination of class isolates collected from a spread of habitats in south west Khasi Hills district of Meghalaya, India. Five totally different primers i.e., M13, enterbacterial repetitive intergenic consensus (ERIC), short tandem repetitive repeats (STRRs) 1A & B and long tandem repetitive repeats (LTRRs), derived from repetitive sequences gift within the ordering of true bacteria, were utilized. The generated knowledge was then evaluated for fingerprinting exploitation procedure and unweighted pairgroup method of arithmetic average (UPGMA) based dendrograms. Clear and variable stripe patterns were obtained for every of the targeted genomic regions used, LTRR being the sole exception. The stripe patterns delineate the existence of 2 basic distinct populations of true bacteria among the 19 isolates. The UPGMA cluster analyses in addition, disclosed the presence of numerous lineages among the isolates. On examination the resolution capability of the four genomic regions studied, it had been seen that, with the exception of LTRR, all the opposite regions hold smart resolution and therefore the ability to differentiate between numerous class populations. The results of the current study indicate the incidence of genetic variants among the 19 morphologically identical isolates of filiform cyanobacteria. Additionally, the study additionally explicates the quality of DNA fingerprinting supplemented with UPGMA cluster analysis as a speedy, economical and value effective tool for differentiating among morphologically identical populations

Spin-phonon coupling suppressing the structural transition in perovskite-like oxide

Multifunctional properties in quantum systems require the interaction between different degrees of freedom. As such, spin-phonon coupling emerges as an ideal mechanism to tune multiferroicity, magnetism, and magnetoelectric response. In this letter, we demonstrate and explain, based on theoretical and experimental analyses, an unusual manifestation of spin-phonon coupling, i.e., prevention of a ferroelastic structural transition, and locking of high-temperature R-3m phase in a magnetically frustrated perovskite-like oxide Ba2NiTeO6. We present Ba2NiTeO6 as a prototype example among its family where long-range antiferromagnetic structure couples with a low-frequency Eg mode (at 55 cm-1) that exhibits a large anharmonicity. Our findings establish that spin-phonon coupling clearly suppresses the phonon anharmonicity preventing the structural phase transition from the R-3m to the C2/m phase in Ba2NiTeO6

Traditional knowledge for dairy animals in Una district of Himachal Pradesh

662-668Traditional knowledge (TK), also known as indigenous knowledge (IK) or local knowledge (LK) generally refers to the matured long-standing traditions and practices of certain regional, indigenous, or local communities. India stands at number 1 in milk production as dairy master across the world. However, due to large number of animal population, distant/remote location, shortage of veterinary staff, some time, veterinary aids are not available to the farmers. In such instances, it is the traditional knowledge of the people which guides them to mitigate the sufferings of the animals. In this article, some of the ITKs are described based on oral communication with the people

Traditional knowledge for dairy animals in Una district of Himachal Pradesh

Traditional knowledge (TK), also known as indigenous knowledge (IK) or local knowledge (LK) generally refers to the matured long-standing traditions and practices of certain regional, indigenous, or local communities. India stands at number 1 in milk production as dairy master across the world. However, due to large number of animal population, distant/remote location, shortage of veterinary staff, some time, veterinary aids are not available to the farmers. In such instances, it is the traditional knowledge of the people which guides them to mitigate the sufferings of the animals. In this article, some of the ITKs are described based on oral communication with the people

Immunoglobulin Class of Leptospira Pomona Antibodies in Cattle

103 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1970.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD

Study of Newbie Fractal Controlled by TAN Function

Abstract I

Study of Newbie Fractal Controlled by SIN Function

Abstract I

<span style="font-size:15.0pt;mso-bidi-font-size: 12.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">A comparative study of immobilized lipase produced from <i style="mso-bidi-font-style:normal">Penicillium chrysogenum</i><span style="mso-bidi-font-style:italic"> SNP5 on two different anionic carriers for its pH and thermostability</span></span>

301-305The present work aimed to increase the thermostability and pH stability of lipase produced from Penicillium chrysogenum SNP5 using different anionic carriers. The immobilized lipase from the fungus on 1.25% sodium alginate beads and 12% polyacrylamide beads retained 37.28 and 59.75% immobilization efficiency, respectively, on 3 mm bead size. Immobilized lipase on calcium alginate and polyacrylamide beads retained 56.16 and 91.11% of its initial activity after 3rd use, whereas significant loss in lipase activity was observed after 5th and 7th reuse, respectively. Properties like optimum pH, Km, Vmax and thermostability of immobilized lipase were changed with respect to free lipase. Optimum pH shifted from 7 to 7.5 in calcium alginate and from 7 to 9 in polyacrylamide beads, whereas t1/2 increased from 71 min to 205 min in calcium alginate and from 71 min to 380 min in polyacrylamide beads at 45°C. Km and Vmax values at free state lipase were observed as 0.30 mM and 31.948 μM min-1 mL-1, whereas after immobilization on calcium alginate and polyacrylamide beads values changed to 0.188 mM and 27.24 μM min-1 mL-1, and 0.24 mM and 30.96 μmol min-1 mL-1, respectively. Thus, on immobilization, lipase has shown enhanced thermostability, specificity and working range of pH, which would be more beneficial for its industrial application. </span

Modulatory effects by neodymium-doped yttrium aluminum garnet laser on fibroblast attachment to single rooted tooth surfaces following ultrasonic scaling and root planning: An in vitro study

Context: One of the most important goals of periodontal therapy is connective tissue reattachment to previously diseased root surfaces. In the recent years, laser therapy has been considered as an important tool in improving the treatment of periodontal disease. Aims: To evaluate the neodymium-doped yttrium aluminum garnet (Nd: YAG) lasers effects on root surfaces affected by periodontal disease and compare this treatment with scaling and root planning (SRP) in terms of fibroblast attachment. Materials and Methods: A sample of 30 single-rooted human teeth extracted because of advanced periodontal disease was used in this study. Sixty specimens obtained by longitudinal sectioning were randomly divided in three groups. Group A control (untreated); Group B SRP; Group C laser (Nd: YAG) and ultrasonic scaling. All specimens were incubated with fibroblast suspension and then fixed and observed under scanning electron microscope. Results: With a median of 8, the control group (Group A) exhibited the least number of total fibroblasts among all the three groups. The laser and scaling - treated group (Group C) showed the highest number of fibroblasts (median = 49, mean ± standard deviation [SD] = 48.28 ± 17.18), followed by SRP only (Group B, median = 22, mean ± SD = 22.24 ± 8.67). Conclusions: Nd: YAG laser irradiation at specific energy densities can be used as a useful tool to condition the root surfaces, enhancing fibroblast attachment. Hence aiding in re-establishment of the connective tissue attachment to the root surfaces of previously diseased teeth