Association Analysis of IL-17A and IL-17F Polymorphisms in Chinese Han Women with Breast Cancer

Background: Research into the etiology of breast cancer has recently focused on the role of the immunity and inflammation. The proinflammatory cytokines IL-17A and IL-17F can mediate inflammation and cancer. To evaluate the influences of IL-17A and IL-17F gene polymorphisms on the risk of sporadic breast cancer, a case-control study was conducted in Chinese Han women. Methodology and Principal Findings: We genotyped three single-nucleotide polymorphisms (SNPs) in IL-17A (rs2275913, rs3819025 and rs3748067) and five SNPs in IL-17F (rs7771511, rs9382084, rs12203582, rs1266828 and rs763780) to determine the haplotypes in 491 women with breast cancer and 502 healthy individuals. The genotypes were determined using the SNaPshot technique. The differences in the genotypic distribution between breast cancer patients and healthy controls were analyzed with the Chi-square test for trends. For rs2275913 in IL-17A, the frequency of the AA genotype was higher in patients than controls (P = 0.0016). The clinical features analysis demonstrated significant associations between IL-17 SNPs and tumor protein 53 (P53), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and triple-negative (ER-/PR-/Her-2-) status. In addition, the haplotype analysis indicated that the frequency of the haplotype A rs2275913G rs3819025G rs3748067, located in the IL-17A linkage disequilibrium (LD) block, was higher in patients than in controls (P = 0.0471 after correction for multiple testing)

Clinical features associated with IL-17A and IL-17F gene polymorphisms.

<p>Clinical features associated with IL-17A and IL-17F gene polymorphisms.</p

Haplotype frequencies in breast cancer patients and healthy controls.

<p>The haplotypes contain the following SNPs in the order listed: IL-17A: rs2275913, rs3819025 and rs3748067; IL-17F: rs763780, rs7771511, rs12203582, rs9382084 and rs1266828. The haplotypes occurred with a frequency of = 10% in the case-control population.</p>a<p><i>P</i> = 0.0471 after correcting the <i>P</i>-value for multiple testing with the Haploview program using 10,000 permutations.</p

Allele frequencies of eight SNPs in the IL-17A and IL-17F genes.

†<p>controls = 501, missing = 1;</p>‡<p>controls = 499, missing = 3.</p>a<p><i>P</i> = 0.0311 after correcting the <i>P</i>-value for multiple testing with the Haploview program using 10,000 permutations.</p

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes