Pre-clinical animal model for ovarian cancer using live imaging system

posterDevelopment of a live imaging animal model for orthotopic ovarian cancers in order to better understand disease mechanisms and response to potential anticancer drug treatments

Transcriptional regulation of the ABCC1/MRP1 by CBF1-dependent Notch1signaling.

Transcriptional regulation of the ABCC1/MRP1 by CBF1-dependent Notch1signaling

Heterarchical SFCS??? ?????? ICPL ????????? ?????? ??????

This paper deals with the design and development of a real-time integrated communication architecture for heterarchical SFCS(Shop Floor Control System). In autonomous agent-based heterarchical SFCS, each functional unit of parts and resources is equipped with an intelligent controller (agent) that acts as the representative of the entity. The controllers communicate and negotiate with other controllers on a real-time basis through message passing and bidding protocol to achieve mutual agreements for task sharing. ICPL(Integrated Communication Protocol and Language) is proposed for this purpose. ICPL is a language and a protocol for supporting communication among intelligent controllers. Based on the speech act theory, this paper proposes a semantic description for ICPL that associates the description of the cognitive states of controllers with the use of language primitives (message_type). Semantics for the basic set of ICPL messages is described. Eventually, an ICPL-based communication architecture can provide the implementation of the distributed and heterarchical SFCS, and makes the intelligent controller transparent to the negotiation problem.clos

Oral Vaccine Delivery for Intestinal Immunity—Biological Basis, Barriers, Delivery System, and M Cell Targeting

Most currently available commercial vaccines are delivered by systemic injection. However, needle-free oral vaccine delivery is currently of great interest for several reasons, including the ability to elicit mucosal immune responses, ease of administration, and the relatively improved safety. This review summarizes the biological basis, various physiological and immunological barriers, current delivery systems with delivery criteria, and suggestions for strategies to enhance the delivery of oral vaccines. In oral vaccine delivery, basic requirements are the protection of antigens from the GI environment, targeting of M cells and activation of the innate immune response. Approaches to address these requirements aim to provide new vaccines and delivery systems that mimic the pathogen’s properties, which are capable of eliciting a protective mucosal immune response and a systemic immune response and that make an impact on current oral vaccine development

Additional file 1: of Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus

Preparation of hIgG1-Fc-9Arg. A. C-terminal 9Arg extension of human IgG1-Fc by PCR. Lane 1. hIgG1-Fc-9Arg PCR (annealing Tm:55, 850 bp), Mw. DNA ladder, lane 2. hIgG1-Fc-9Arg PCR (annealing Tm:52, 850 bp). Template for PCR was an Avastin heavy chain. B. DNA sequence analysis of hIgG1-Fc-9Arg expression vector. C. SDS-PAGE with reduced hIgG1-Fc-9Arg. Mw. protein ladder (10–245 kDa), lane1. 0.1 μg, lane 2. one μg, land 3. ten μg and PAGE with non-reduced hIgG1-Fc-9Arg (lane 1) and Mw (protein ladder (10–245 kDa)). (PPTX 314 kb

Design and Characterization of Bioengineered Cancer-Like Stem Cells

<div><p>Cancer stem cells (CSCs) are a small subset of cancer cells responsible for maintenance and progression of several types of cancer. Isolation, propagation, and the differentiation of CSCs in the proper stem niches expose the intrinsic difficulties for further studies. Here we show that induced cancer like stem cells (iCLSCs) can be generated by <i>in vitro</i> oncogenic manipulation of mouse embryonic stem cells (mESCs) with well-defined oncogenic elements; SV40 LTg and H<i>ras</i>V12 by using a mouse stem virus long terminal repeat (MSCV-LTR)-based retroviral system. The reprogrammed mESCs using both oncogenes were characterized through their oncogenic gene expression, the enhancement of proliferation, and unhampered maintenance of stem properties <i>in vitro</i> and <i>in vivo</i>. In addition, these transformed cells resulted in the formation of malignant, immature ovarian teratomas <i>in viv</i>o. To successfully further expand these properties to other organs and species, more research needs to be done to fully understand the role of a tumor- favorable microenvironment. Our current study has provided a novel approach to generate induced cancer like stem cells through <i>in vitro</i> oncogenic reprogramming and successfully initiated organ-specific malignant tumor formation in an orthotopic small animal cancer model.</p></div

Characterization of genetically modified, retrovirally transduced mESCs.

<p>Representative images from immunoblot analysis: (A) H<i>ras</i>V12, (B) SV40 LTg. (C) CCK cell proliferation assay for 7 days of proliferation period of mESCs and transformed mESCs. (D) Comparison of proliferations at day 7. Mean ± S.D. (n = 3), *, **, and #, ## p<0.05. ANOVA test was performed with Tukey’s post-test using the GraphPad Prism software.</p

Additional file 3: of Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus

Endosomal trafficking of hIgG1-Fc-9Arg complex A. Cellular uptake and endosomal escape of FITC-hIgG1-Fc-9Arg in Caco-2 cells. B. Determination of bobo-3-pDNA complexed with hIgG1-Fc-9Arg on 50/1 weight ratio for tracking complexes mediated FcRn and evaluation of presence pDNA in Caco-2 cells. Lipofectamine used as positive control. Scale bar is 50 Οm. (PPTX 1263 kb