2,627 research outputs found
Electrical Investigation of the Oblique Hanle Effect in Ferromagnet/Oxide/Semiconductor Contacts
We have investigated the electrical Hanle effect with magnetic fields applied
at an oblique angle ({\theta}) to the spin direction (the oblique Hanle effect,
OHE) in CoFe/MgO/semiconductor (SC) contacts by employing a three-terminal
measurement scheme. The electrical oblique Hanle signals obtained in
CoFe/MgO/Si and CoFe/MgO/Ge contacts show clearly different line shapes
depending on the spin lifetime of the host SC. Notably, at moderate magnetic
fields, the asymptotic values of the oblique Hanle signals (in both contacts)
are consistently reduced by a factor of cos^2({\theta}) irrespective of the
bias current and temperature. These results are in good agreement with
predictions of the spin precession and relaxation model for the electrical
oblique Hanle effect. At high magnetic fields where the magnetization of CoFe
is significantly tilted from the film plane to the magnetic field direction, we
find that the observed angular dependence of voltage signals in the CoFe/MgO/Si
and CoFe/MgO/Ge contacts are well explained by the OHE, considering the
misalignment angle between the external magnetic field and the magnetization of
CoFe.Comment: 19 pages, 8 figure
Electrical spin injection and accumulation in CoFe/MgO/Ge contacts at room temperature
We first report the all-electrical spin injection and detection in
CoFe/MgO/moderately doped n-Ge contact at room temperature (RT), employing
threeterminal Hanle measurements. A sizable spin signal of ~170 k{\Omega}
{\mu}m^2 has been observed at RT, and the analysis using a single-step
tunneling model gives a spin lifetime of ~120 ps and a spin diffusion length of
~683 nm in Ge. The observed spin signal shows asymmetric bias and temperature
dependences which are strongly related to the asymmetry of the tunneling
process.Comment: 28 pages, 5 figure
Soft X-ray resonant Kerr rotation measurement and simulation of element-resolved and interface-sensitive magnetization reversals in a NiFe/FeMn/Co trilayer structure
We report experimental observations of element- and buried interface-resolved magnetization reversals in an oppositely exchange-biased NiFeFeMnCo trilayer structure by soft x-ray resonant Kerr rotation measurements. Not only Co-, Ni-, Fe-specific exchange-biased loops but also interfacial uncompensated (UC) Fe reversal loops coupled to the individual Co and NiFe layers are separately observed. From the experimental results interpreted with the help of the model simulations of soft x-ray resonant Kerr rotation, the effective thicknesses of interfacial UC regions at the buried interfaces of both FeMnCo and NiFeFeMn are found to be tUC =13??2 A and 6??4 A, respectively. The depth sensitivity as well as element specificity of the x-ray resonant Kerr effect offer an elegant way into the investigations of element- and depth-resolved magnetization reversals of ferromagnetic ultrathin regions at buried interfaces in multicomponent multilayer films.open91
Transvaginal Endoscopic Appendectomy
Since Kalloo and colleagues first reported the feasibility and safety of a peroral transgastric approach in the porcine model in 2004, various groups have reported more complex natural orifice transluminal endoscopic surgery (NOTES) procedures, such as the cholecystectomy, splenectomy and liver biopsy, in the porcine model. Natural orifice access to the abdominal cavity, such as transgastric, transvesical, transcolonic, and transvaginal, has been described. Although a novel, minimally invasive approach to the abdominal cavity is a peroral endoscopic transgastric approach, there are still some challenging issues, such as the risk of infection and leakage, and the method of gastric closure. Hybrid-NOTES is an ideal first step in humans. Human hybrid transvaginal access has been used for years by many surgeons for diagnostic and therapeutic purposes. Here, we report a transvaginal flexible endoscopic appendectomy, with a 5-mm umbilical port using ultrasonic scissors in a 74-year-old woman with acute appendicitis
Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A
Hepatitis C virus (HCV) utilizes autophagy to promote its propagation. Here we show the autophagy-mediated suppression of HCV replication via the endoplasmic reticulum (ER) protein SCOTIN. SCOTIN overexpression inhibits HCV replication and infectious virion production in cells infected with cell culture-derived HCV. HCV nonstructural 5A (NS5A) protein, which is a critical factor for HCV RNA replication, interacts with the IFN-beta-inducible protein SCOTIN, which transports NS5A to autophagosomes for degradation. Furthermore, the suppressive effect of SCOTIN on HCV replication is impaired in both ATG7-silenced cells and cells treated with autophagy or lysosomal inhibitors. SCOTIN does not affect the overall flow of autophagy; however, it is a substrate for autophagic degradation. The physical association between the transmembrane/proline-rich domain (TMPRD) of SCOTIN and Domain-II of NS5A is essential for autophagosomal trafficking and NS5A degradation. Altogether, our findings suggest that IFN-beta-induced SCOTIN recruits the HCV NS5A protein to autophagosomes for degradation, thereby restricting HCV replication.1110Ysciescopu
Akt regulates the expression of MafK, synaptotagmin I, and syntenin-1, which play roles in neuronal function
<p>Abstract</p> <p>Background</p> <p>Akt regulates various cellular processes, including cell growth, survival, and metabolism. Recently, Akt's role in neurite outgrowth has also emerged. We thus aimed to identify neuronal function-related genes that are regulated by Akt.</p> <p>Methods</p> <p>We performed suppression subtractive hybridization on two previously established PC12 sublines, one of which overexpresses the wild-type (WT) form and the other, the dominant-negative (DN) form of Akt. These sublines respond differently to NGF's neuronal differentiation effect.</p> <p>Results</p> <p>A variety of genes was identified and could be classified into several functional groups, one of which was developmental processes. Two genes involved in neuronal differentiation and function were found in this group. v-Maf musculoaponeurotic fibrosarcoma oncogene homolog K (MafK) induces the neuronal differentiation of PC12 cells and immature telencephalon neurons, and synaptotagmin I (SytI) is essential for neurotransmitter release. Another gene, <it>syntenin-1 </it>(<it>Syn-1</it>) was also recognized in the same functional group into which <it>MafK </it>and <it>SytI </it>were classified. Syn-1 has been reported to promote the formation of membrane varicosities in neurons. Quantitative reverse transcription polymerase chain reaction analyses show that the transcript levels of these three genes were lower in PC12 (WT-Akt) cells than in parental PC12 and PC12 (DN-Akt) cells. Furthermore, treatment of PC12 (WT-Akt) cells with an Akt inhibitor resulted in the increase of the expression of these genes and the improvement of neurite outgrowth. These results indicate that dominant-negative or pharmacological inhibition of Akt increases the expression of <it>MafK</it>, <it>SytI</it>, and <it>Syn-1 </it>genes. Using lentiviral shRNA to knock down endogenous Syn-1 expression, we demonstrated that Syn-1 promotes an increase in the numbers of neurites and branches.</p> <p>Conclusions</p> <p>Taken together, these results indicate that Akt negatively regulates the expression of <it>MafK</it>, <it>SytI</it>, and <it>Syn-1 </it>genes that all participate in regulating neuronal integrity in some way or another.</p
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