Tissue reaction against implantation of nanocomposite and giomers

Dentistry today has inherited technological advancement from other Dental Material sciences, examples are Nanocomposite and Giomers. Nanocomposites and Giomers are common materials used in Dentistry. But what are the implications when these materials are used in practice? The aim for this the study shows the analysis of tissue reaction due to implantation of Nanocomposite and Giomers. The subcutaneous tissue of a mouse is substituted with the human gum tissues. In the experimental group, Nanocomposite and Giomers were implanted in the subcutaneous tissue abdomen region in mice. The slides were made from the surrounding of implantation for both experimental and control groups. The evaluation of the effects of the implant is done in a time interval. Evaluated time intervals are 24 hours, 7" day, 14th day, 21st day and 28th day respectively. The amounts of inflammatory cells formation in both groups were compared.Once the results of the inflammatory cells are evaluated in the given time interval for Nanocomposite, Giomer, and control group then they are statistically analyzed. The statistics used in the experiments is Mann-Whitney and Wilcoxon. The conclusion of this research showed that statistically significant differences on lymphocytes value between treatment and control group

Immunoexpression of cytokeratin 19 in oral swab from fixed orthodontic appliance users

The use of fixed orthodontic appliances can improve someone's mastication, speech and appearance. However, this appliance acts as a strange object that may cause irritation to the mucosa epithelial of oral cavity, because of the friction and pressure from the components of the fixed orthodontic appliances which are in direct contact with the oral mucosa. Irritation in the oral mucosa could stimulate the increase of cytokeratin. The appearance of cytokeratin is then used to identify the condition of these cells. This study was a descriptive research to find the expression of cytokeratin 19 with immunohistochemical method in oral mucosa epithelial of fixed orthodontic appliances users. Sample in this study was chosen from 30 fixed orthodontic appliances users. The result of this study was determined by calculating the number of positive cells (brown), compared with total number of cells. The account of positive cells would present the reaction of the epithelial cells according to the inflamation stage which caused by the use of orthodontic appliances. As a conclusion of this study, the use of fixed orthodontic appliances may cause changes in epithelial mucosa which form an adaptation process by increasing the number of progenitor cells marked by cytokeratin 19

Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini

Histologic localization of PLAG1 (pleomorphic adenoma gene 1) in pleomorphic adenoma of the salivary gland: cytogenetic evidence of common origin of phenotypically diverse cells.

Pleomorphic adenoma gene 1 (PLAG1), a zinc finger transcription factor gene, is consistently rearranged and overexpressed in human pleomorphic adenomas of the salivary glands with 8q12 translocations. In this report, we describe the immunohistochemical localization of PLAG1 protein in pleomorphic adenomas of the salivary gland and corresponding normal tissue, in relation to cytokeratin, vimentin, and BCL-2 expression. Normal salivary gland tissue was not immunoreactive for PLAG1. In primary pleomorphic adenomas, cells strongly immunoreactive for PLAG1 were detected in the outer layer of tubulo-ductal structures, which are thought to be the origin of cells with bi-directional, epithelial, and mesenchymal phenotypes. In contrast, epithelial cells with abundant cytokeratin in the inner tubulo-ductal structures only sporadically expressed PLAG1. BCL-2 immunoreactivity was found mainly in the cells surrounding the tubulo-ductal structures and in the solid undifferentiated cellular masses, within the areas that had moderate PLAG1 immunoreactivity. The variability of PLAG1 expression in neoplastic cells seemed to reflect the morphologic heterogeneity that correlated with the stage of differentiation of the tumor cells. Immunohistochemical/cytogenetic evaluation of two pleomorphic adenomas with t(3;8)(p21;q12) or t(5;8)(p13;q12) translocations demonstrated the clonal nature of immunophenotypically diverse cells. This finding confirms the theory that pleomorphic adenoma cells share a common single-cell origin, most likely from the epithelial progenitor basal duct cells