3DFusion, A real-time 3D object reconstruction pipeline based on streamed instance segmented data

This paper presents a real-time segmentation and reconstruction system that utilizes RGB-D images to generate accurate and detailed individual 3D models of objects within a captured scene. Leveraging state-of-the-art instance segmentation techniques, the system performs pixel-level segmentation on RGB-D data, effectively separating foreground objects from the background. The segmented objects are then reconstructed into distinct 3D models in a high-performance computation platform. The real-time 3D modelling can be applied across various domains, including augmented/virtual reality, interior design, urban planning, road assistance, security systems, and more. To achieve real-time performance, the paper proposes a method that effectively samples consecutive frames to reduce network load while ensuring reconstruction quality. Additionally, a multi-process SLAM pipeline is adopted for parallel 3D reconstruction, enabling efficient cutting of the clustering objects into individuals. This system employs the industry-leading framework YOLO for instance segmentation. To improve YOLO's performance and accuracy, modifications were made to resolve duplicated or false detection of similar objects, ensuring the reconstructed models align with the targets. Overall, this work establishes a robust real-time system with a significant enhancement for object segmentation and reconstruction in the indoor environment. It can potentially be extended to the outdoor scenario, opening up numerous opportunities for real-world applications

Optothermal Microbubble Assisted Manufacturing of Nanogap-Rich Structures for Active Chemical Sensing

Guiding analytes to the sensing area is an indispensable step in a sensing system. Most of the sensing systems apply a passive sensing method, which waits for the analytes to diffuse towards the sensor. However, passive sensing methods limit the detection of analytes to a picomolar range on micro/nanosensors for a practical time scale. Therefore, active sensing methods need to be used to improve the detection limit in which the analytes are forced to concentrate on the sensors. In this article, we have demonstrated the manufacturing of nanogap-rich structures for active chemical sensing. Nanogap-rich structures are manufactured from metallic nanoparticles through an optothermally generated microbubble (OGMB) which is a laser-induced micron-sized bubble. The OGMB induces a strong convective flow that helps to deposit metallic nanoparticles to form nanogap-rich structures on a solid surface. In addition, the OGMB is used to guide and concentrate analytes towards the nanogap-rich structures for the active sensing of analytes. An active sensing method can improve the detection limit of chemical substances by an order of magnitude compared to a passive sensing method. The microbubble assisted manufacturing of nanogap-rich structures together with an active analyte sensing method paves a new way for advanced chemical and bio-sensing applications

The Impact of Oxygen on Bacterial Enteric Pathogens

Bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. During their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. However, the majority of enteric infection research has been conducted under aerobic conditions. To raise awareness of the importance in understanding the impact of oxygen, or lack of oxygen, on enteric pathogenesis, we describe in this review the metabolic and physiological responses of nine bacterial enteric pathogens exposed to environments with different oxygen levels. We further discuss the effects of oxygen levels on virulence regulation to establish potential connections between metabolic adaptations and bacterial pathogenesis. While not providing an exhaustive list of all bacterial pathogens, we highlight key differences and similarities among nine facultative anaerobic and microaerobic pathogens in this review to argue for a more in-depth understanding of the diverse impact oxygen levels have on enteric pathogenesis

Multislice CT Angiography of Fenestrated Endovascular Stent Grafting for Treating Abdominal Aortic Aneurysms: a Pictorial Review of the 2D/3D Visualizations

Fenestrated endovascular repair of an abdominal aortic aneurysm has been developed to treat patients with a short or complicated aneurysm neck. Fenestration involves creating an opening in the graft fabric to accommodate the orifice of the vessel that is targeted for preservation. Fixation of the fenestration to the renal arteries and the other visceral arteries can be done by implanting bare or covered stents across the graft-artery ostia interfaces so that a portion of the stent protrudes into the aortic lumen. Accurate alignment of the targeted vessels in a longitudinal aspect is hard to achieve during stent deployment because rotation of the stent graft may take place during delivery from the sheath. Understanding the 3D relationship of the aortic branches and the fenestrated vessel stents following fenestration will aid endovascular specialists to evaluate how the stent graft is situated within the aorta after placement of fenestrations. The aim of this article is to provide the 2D and 3D imaging appearances of the fenestrated endovascular grafts that were implanted in a group of patients with abdominal aortic aneurysms, based on the multislice CT angiography. The potential applications of each visualization technique were explored and compared with the 2D axial images

Multislice CT angiography in the follow-up of fenestrated endovascular grafts: Effect of slice thickness on 2D and 3D visualization of the fenestrated stents

Purpose: To investigate the effect of multislice computed tomography (CT) protocols on the visualization of target vessel stents in patients with treated with fenestrated endovascular grafts.Methods: Twenty-one patients (19 men; mean age 75 years, range 63?86) undergoing fenestrated endovascular repair of abdominal aortic aneurysm (AAA) were retrospectively studied. Multislice CT angiography was performed with several protocols, and the section thicknesses used in each were compared to identify any relationship between slice thickness and target vessel stents visualized on 2-dimensional (2D) axial, multiplanar reformatted (MPR), and 3-dimensional (3D) virtual intravascular endoscopy (VIE) images. Image quality was assessed based on the degree of artifacts and their effect on the ability to visualize the configuration, intra-aortic location, and intraluminal appearance of the target vessel stents and measure their protrusion into the aortic lumen.Results: There were 7 different multislice CT scanning protocols employed in the 21 patients (25 datasets, with 2 sets of follow-up images in 4 patients). The slice thicknesses and numbers (n) of studies included were 0.5 (n=3), 0.625 (n=6), 1.0 (n=1), 1.25 (n=9), 2.5 (n=3), 3.0 (n=1), and 5.0 mm (n=2). Of these CT protocols, images (especially 2D/3D reconstructions) acquired at 2.5, 3.0, and 5.0 mm were significantly compromised by interference from artifacts. Images acquired with a slice thickness of 1.0 or 1.25 mm were scored equally or lower than those acquired with a submillimeter section thickness (0.5 or 0.625 mm), with minor degrees of artifacts resulting in acceptable image quality.Conclusion: Visualization of the target vessel stents depends on the appropriate selection of multislice CT scanning protocols. Our results showed that studies performed with a slice thickness of 1.0 or 1.25 mm produced similar image quality to those with a thickness of 0.5 or 0.625 mm. Submillimeter slices are not recommended in imaging patients treated with fenestrated stent-grafts, as they did not add additional information to the visualization

Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates

Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts have focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate to detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, and an understanding of bioconjugate stability is needed. Here, we show a method for optimizing Listeria monocytogenes polyclonal antibodies bioconjugation mechanisms to AuNPs via covalent binding at different pH values, from 2 to 11, and 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid, NaOH, HCl conditions. By fitting Lorentz curves to the amide I and II regions, we analyze the stability of the antibody secondary structure. This shows an increase in the apparent breakdown of the antibody secondary structure during bioconjugation as pH decreases from 7.9 to 2. We find variable adsorption efficiency, measured as the percentage of antibody adsorbed to the AuNP surface, from 17 to 27% as pH increases from 2 to 6 before decreasing to 8 and 13% at pH 7.9 and 11, respectively. Transmission electron microscopy (TEM) analysis reveals discrepancies between size and morphological changes due to the corona layer assembly from antibody binding to single nanoparticles versus aggregation or cluster self-assembly into large aggregates. The corona layer formation size increases from 3.9 to 5.1 nm from pH 2 to 6, at pH 7.9, there is incomplete corona formation, whereas at pH 11, there is a corona layer formed of 6.4 nm. These results indicate that the covalent binding process was more efficient at lower pH values; however, aggregation and deactivation of the antibodies were observed. We demonstrate that optimum bioconjugation condition was determined at pH 6 and MES buffer-type by indicators of covalent bonding and stability of the antibody secondary structure using Fourier transform-infrared, the morphological characteristics and corona layer formation using TEM, and low wavelength shifts of ultraviolet–visible after bioconjugation

CT virtual intravascular endoscopy in the follow-up of endoluminal fenestrated stent grafts

Purpose: To investigate the potential value of CT virtual intravascular endoscopy in the follow-up of patients with abdominal aortic aneurysms treated with endoluminal fenestrated stent grafts.Methods and Materials: Eight patients with abdominal aortic aneurysms unsuitable for open surgery or conventional endovascular repair undergoing fenestrated stent grafts were included in the study. Both pre-and post-fenestration multislice CT data were collected in these patients and used for generation of virtual intravascular endoscopy images of aortic ostium and endovascular stents. Virtual endoscopy images were compared with conventional axial CT and multiplanar images with the aim of identifying the appearances of the endoluminal stents. The length of endoluminal stents that protruded into the aortic lumen was measured on virtual endoscopy images.Results: Variable fenestrations were deployed in 27 aortic branches with scallop fenestration implanted in 5 aortic ostia, large fenestration in 7 aortic ostia and small fenestration in 15 renal ostia, respectively. All of the fenestrated branches remained patent. Virtual endoscopy was superior to conventional 2D images in the visualization of configuration of endovascular stents. Most of the stents (68%) were found to be circular visualized on virtual endoscopy images, while the remaining stents were irregular in terms of endoluminal appearances. 95% of these stents were shown to protrude into the lumen with length less than 7.0 mm. There is no significant difference of the diameters of aortic ostia between pre-and post- fenestration (p>0.05).Conclusion: Our preliminary study shows that virtual intravascular endoscopy could be a valuable technique to follow-up patients treated with endoluminal fenestrated stent grafts

Detection and Aggregation of Listeria monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates

Magnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs) as surface-enhanced Raman spectroscopy (SERS) probes. Listeria monocytogenes were used as the pathogenic bacteria and the pAb-Lis-AuMNs, 300 nm diameter, were used as probes allowing facile magnetic separation and aggregation. An optimized covalent bioconjugation procedure between the magnetic nanoshells and the polyclonal antibody was performed at pH six via a carbodiimide crosslinking reaction. Spectroscopic and morphological characterization techniques confirmed the fabrication of stable pAb-Lis-AuMNs. The resulting pAb-Lis-AuMNs acted as a SERS probe for L. monocytogenes based on the targeted capture via surface binding interactions and magnetically induced aggregation. Label-free SERS measurements were recorded for the minimum detectable amount of L. monocytogenes based on the SERS intensity at the 1388 cm−1 Raman shift. L. monocytogenes concentrations exhibited detection limits in the range of 104–107 CFU ml−1, before and after aggregation. By fitting these concentrations, the limit of detection of this method was ∼103 CFU ml−1. Using a low-intensity magnetic field of 35 G, pAb-Lis-AuMNs aggregated L. monocytogenes as demonstrated with microscopy techniques, including SEM and optical microscopy. Overall, this work presents a label-free SERS probe method comprised of a surface-modified polyclonal antibody sub-micron magnetic nanoshell structures with high sensitivity and magnetic induced separation that could lead to the fabrication of multiple single-step sensors

Computer simulation and analysis of hemodynamic changes in abdominal aortic aneurysms treated with fenestrated endovascular grafts

The purpose of this study was to perform a simulation of blood flow and analyze the hemodynamic changes in patients with abdominal aortic aneurysms (AAA) treated with fenestrated stent grafts. Four patients with AAA undergoing multislice computed tomography angiography pre-and post-fenestrated stent graft implantation were selected for inclusion in the study. Geometric models and hexahedral volume meshes were successfully generated for pre- and post-stent fenestrated implantation. The blood flow pattern was simulated inside the abdominal aortic aneurysm and arterial branches, as well as with a stentgraft in situ. Flow visualization showed that flow disturbances inside the aneurysm were apparently decreased and flow rate was not affected significantly at the renal arteries after deployment of the fenestrated stents into these branches. The wall pressure was found to reduce inside the aneurysm sac following implantation of stent grafts. In this preliminary study, we successfully simulated the flow characteristics in abdominal aortic aneurysm before and after fenestrated endovascular repair

High Seroprevalence Against Typhus Group and Spotted Fever Group Rickettsiae in Rural Indigenous Populations of Peninsular Malaysia

Rickettsioses of the typhus group (TG) and spotted fever group (SFG) are emerging bacterial infections worldwide, especially in the tropics. Only a few studies on these pathogens and their respective clinical diseases have been conducted in Malaysia. Here, we performed a seroprevalence study among 544 healthy, afebrile indigenous people (Orang Asli) from peninsular Malaysia for TG and SFG rickettsioses in nine rural and peri-urban settlements. The study population encompassed children, adolescents, and adults. The overall seroprevalence of rickettsiosis in the Orang Asli was 48.5%, with 27.9% seroprevalence against TG rickettsiae and 20.6% seroprevalence against SFG rickettsiae. In 7.9% of the study participants, antibodies against both rickettsial groups were found. The highest seropositivity rates against TG and SRG rickettsiae were detected in young children and adults. Overall, there were no gender differences. Seroprevalences were similar among inhabitants of different settlements, except for two localities. More studies are needed to shed more light on the ecology and risk factors for TG and SFG rickettsioses in Malaysia