Effect of bone morphogenetic protein-2 on diabetic retinopathy and its mechanism of action

Purpose: To investigate the effect of bone morphogenetic protein-2 (BMP-2) on human retinal vascular endothelial cells (RECs) and human retinal pigment epithelial cells (RPE) cultured in high glucose (HG) in vitro, and the underlying mechanism. Methods: Cell counting kit-8 (CCK-8) was used to determine cell proliferation while Western blot was used to assay the expressions of extracellular matrix and angiogenesis-related factors, Expressions of cytokines and chemokines were assessed by quantitative real time polymerase chain reaction (qRTPCR) and enzyme-linked immunosorbent assay (ELISA). Changes in Smad, ERK, JNK and p38MAPK signal pathway were measured by transfection and interference. Results: The level of expression of BMP-2 in HG group was higher than that in normal glucose (NG) culture group. The expressions of angiogenesis-related factors i.e. vascular endothelial growth factor (VEGF) and intercellular cell adhesion molecule-1 (ICAM1), pro-inflammatory factors i.e. IL-6 and chemokine monocyte chemokine protein-1 (MCP1), increased significantly in HG group compared to NG and HG + BMP-2 groups. Phosphorylation of Smad1/5/8 and activation of ERK, JNK and p38MAPK signaling pathways were enhanced by BMP-2. Conclusion: These results suggest that BMP-2 promotes angiogenesis and enhances the expressions of inflammatory cytokines via Smad signaling pathway

Hyperbolic Circle Packings and Total Geodesic Curvatures on Surfaces with Boundary

This paper investigates a generalized hyperbolic circle packing (including circles, horocycles or hypercycles) with respect to the total geodesic curvatures on the surface with boundary. We mainly focus on the existence and rigidity of circle packing whose contact graph is the $1$-skeleton of a finite polygonal cellular decomposition, which is analogous to the construction of Bobenko and Springborn [4]. Motivated by Colin de Verdi\`ere's method [6], we introduce the variational principle for generalized hyperbolic circle packings on polygons. By analyzing limit behaviours of generalized circle packings on polygons, we give an existence and rigidity for the generalized hyperbolic circle packing with conical singularities regarding the total geodesic curvature on each vertex of the contact graph. As a consequence, we introduce the combinatoral Ricci flow to find a desired circle packing with a prescribed total geodesic curvature on each vertex of the contact graph.Comment: 26 pages, 7 figure

Distributed State Fusion Kalman Predictor Based on WLS Algorithm

AbstractBased on the weighted least square (WLS) algorithm, a unified multisensor distributed state fusion estimation criterion is presented in this paper, which includes the optimal weighted fusion algorithms weighted by matrices, scalars and diagonal matrices as the special cases. Furthermore, the corresponding optimal distributed state fusion Kalman predictor is presented for the multisensor linear discrete time-varying stochastic control systems with different measurement matrices and correlated noises. A simulation example for a tracking system with 3-sensor shows its effectiveness