2,972 research outputs found
A diagnostic real-time PCR assay for the rapid identification of the tomato-potato psyllid, Bactericera cockerelli (Sulc, 1909) and development of a psyllid barcoding database
The accurate and rapid identification of insect pests is an important step in the prevention and control of outbreaks in areas that are otherwise pest free. The potato-tomato psyllid Bactericera cockerelli (Sulc, 1909) is the main vector of 'Candidatus Liberibacter solanacearum' on potato and tomato crops in North America and New Zealand; and is considered a threat for introduction in Europe and other pest-free regions. This study describes the design and validation of the first species-specific TaqMan probe-based real-time PCR assay, targeting the ITS2 gene region of B. cockerelli. The assay detected B. cockerelli genomic DNA from adults, immatures, and eggs, with 100% accuracy. This assay also detected DNA from cloned plasmids containing the ITS2 region of B. cockerelli with 100% accuracy. The assay showed 0% false positives when tested on genomic and cloned DNA from 73 other psyllid species collected from across Europe, New Zealand, Mexico and the USA. This included 8 other species in the Bactericera genus and the main vectors of 'Candidatus Liberibacter solanacearum' worldwide. The limit of detection for this assay at optimum conditions was 0.000001ng DNA (similar to 200 copies) of ITS2 DNA which equates to around a 1:10000 dilution of DNA from one single adult specimen. This assay is the first real-time PCR based method for accurate, robust, sensitive and specific identification of B. cockerelli from all life stages. It can be used as a surveillance and monitoring tool to further study this important crop pest and to aid the prevention of outbreaks, or to prevent their spread after establishment in new areas
TIME Impact - a new user-friendly tuberculosis (TB) model to inform TB policy decisions.
Tuberculosis (TB) is the leading cause of death from infectious disease worldwide, predominantly affecting low- and middle-income countries (LMICs), where resources are limited. As such, countries need to be able to choose the most efficient interventions for their respective setting. Mathematical models can be valuable tools to inform rational policy decisions and improve resource allocation, but are often unavailable or inaccessible for LMICs, particularly in TB. We developed TIME Impact, a user-friendly TB model that enables local capacity building and strengthens country-specific policy discussions to inform support funding applications at the (sub-)national level (e.g. Ministry of Finance) or to international donors (e.g. the Global Fund to Fight AIDS, Tuberculosis and Malaria).TIME Impact is an epidemiological transmission model nested in TIME, a set of TB modelling tools available for free download within the widely-used Spectrum software. The TIME Impact model reflects key aspects of the natural history of TB, with additional structure for HIV/ART, drug resistance, treatment history and age. TIME Impact enables national TB programmes (NTPs) and other TB policymakers to better understand their own TB epidemic, plan their response, apply for funding and evaluate the implementation of the response.The explicit aim of TIME Impact's user-friendly interface is to enable training of local and international TB experts towards independent use. During application of TIME Impact, close involvement of the NTPs and other local partners also builds critical understanding of the modelling methods, assumptions and limitations inherent to modelling. This is essential to generate broad country-level ownership of the modelling data inputs and results. In turn, it stimulates discussions and a review of the current evidence and assumptions, strengthening the decision-making process in general.TIME Impact has been effectively applied in a variety of settings. In South Africa, it informed the first South African HIV and TB Investment Cases and successfully leveraged additional resources from the National Treasury at a time of austerity. In Ghana, a long-term TIME model-centred interaction with the NTP provided new insights into the local epidemiology and guided resource allocation decisions to improve impact
A Mission to Explore the Pioneer Anomaly
The Pioneer 10 and 11 spacecraft yielded the most precise navigation in deep
space to date. These spacecraft had exceptional acceleration sensitivity.
However, analysis of their radio-metric tracking data has consistently
indicated that at heliocentric distances of astronomical units,
the orbit determinations indicated the presence of a small, anomalous, Doppler
frequency drift. The drift is a blue-shift, uniformly changing with a rate of
Hz/s, which can be interpreted as a
constant sunward acceleration of each particular spacecraft of . This signal has become known as the Pioneer
anomaly. The inability to explain the anomalous behavior of the Pioneers with
conventional physics has contributed to growing discussion about its origin.
There is now an increasing number of proposals that attempt to explain the
anomaly outside conventional physics. This progress emphasizes the need for a
new experiment to explore the detected signal. Furthermore, the recent
extensive efforts led to the conclusion that only a dedicated experiment could
ultimately determine the nature of the found signal. We discuss the Pioneer
anomaly and present the next steps towards an understanding of its origin. We
specifically focus on the development of a mission to explore the Pioneer
Anomaly in a dedicated experiment conducted in deep space.Comment: 8 pages, 9 figures; invited talk given at the 2005 ESLAB Symposium
"Trends in Space Science and Cosmic Vision 2020", 19-21 April 2005, ESTEC,
Noordwijk, The Netherland
Fundamental Physics with the Laser Astrometric Test Of Relativity
The Laser Astrometric Test Of Relativity (LATOR) is a joint European-U.S.
Michelson-Morley-type experiment designed to test the pure tensor metric nature
of gravitation - a fundamental postulate of Einstein's theory of general
relativity. By using a combination of independent time-series of highly
accurate gravitational deflection of light in the immediate proximity to the
Sun, along with measurements of the Shapiro time delay on interplanetary scales
(to a precision respectively better than 0.1 picoradians and 1 cm), LATOR will
significantly improve our knowledge of relativistic gravity. The primary
mission objective is to i) measure the key post-Newtonian Eddington parameter
\gamma with accuracy of a part in 10^9. (1-\gamma) is a direct measure for
presence of a new interaction in gravitational theory, and, in its search,
LATOR goes a factor 30,000 beyond the present best result, Cassini's 2003 test.
The mission will also provide: ii) first measurement of gravity's non-linear
effects on light to ~0.01% accuracy; including both the Eddington \beta
parameter and also the spatial metric's 2nd order potential contribution (never
measured before); iii) direct measurement of the solar quadrupole moment J2
(currently unavailable) to accuracy of a part in 200 of its expected size; iv)
direct measurement of the "frame-dragging" effect on light by the Sun's
gravitomagnetic field, to 1% accuracy. LATOR's primary measurement pushes to
unprecedented accuracy the search for cosmologically relevant scalar-tensor
theories of gravity by looking for a remnant scalar field in today's solar
system. We discuss the mission design of this proposed experiment.Comment: 8 pages, 9 figures; invited talk given at the 2005 ESLAB Symposium
"Trends in Space Science and Cosmic Vision 2020," 19-21 April 2005, ESTEC,
Noodrwijk, The Netherland
Transcriptomic Profiling in Childhood H1N1/09 Influenza Reveals Reduced Expression of Protein Synthesis Genes
We compared the blood RNA transcriptome of children hospitalized with influenza A H1N1/09, respiratory syncytial virus (RSV) or bacterial infection, and healthy controls. Compared to controls, H1N1/09 patients showed increased expression of inflammatory pathway genes and reduced expression of adaptive immune pathway genes. This was validated on an independent cohort. The most significant function distinguishing H1N1/09 patients from controls was protein synthesis, with reduced gene expression. Reduced expression of protein synthesis genes also characterized the H1N1/09 expression profile compared to children with RSV and bacterial infection, suggesting that this is a key component of the pathophysiological response in children hospitalized with H1N1/09 infection
The Polarizing Power of the Interstellar Medium in Taurus
We present a study of the polarizing power of the dust in cold dense regions
(dark clouds) compared to that of dust in the general interstellar medium
(ISM). Our study uses new polarimetric, optical, and spectral classification
data for 36 stars to carefully study the relation between polarization
percentage (p) and extinction (A_V) in the Taurus dark cloud complex. We find
two trends in our p-A_V study: (1) stars background to the warm ISM show an
increase in p with A_V; and (2) the percentage of polarization of stars
background to cold dark clouds does not increase with extinction. We detect a
break in the p-A_V relation at an extinction 1.3 +/- 0.2 mag, which we expect
corresponds to a set of conditions where the polarizing power of the dust
associated with the Taurus dark clouds drops precipitously. This breakpoint
places important restrictions on the use of polarimetry in studying
interstellar magnetic fields.Comment: 17 pages, 2 figures, to appear in ApJLett, AASTeX was use
First Constraints on Source Counts at 350 Microns
We have imaged a 6 arcminute region in the Bo\"otes Deep Field
using the 350 m-optimised second generation Submillimeter High Angular
Resolution Camera (SHARC II), achieving a peak 1 sensitivity of 5
mJy. We detect three sources above 3, and determine a spurious source
detection rate of 1.09 in our maps. In the absence of detections, we
rely on deep 24 m and 20 cm imaging to deduce which sources are most
likely to be genuine, giving two real sources. From this we derive an integral
source count of 0.84 sources arcmin at mJy,
which is consistent with 350 m source count models that have an
IR-luminous galaxy population evolving with redshift. We use these constraints
to consider the future for ground-based short-submillimetre surveys.Comment: accepted for publication in The Astrophysical Journa
Bumblebee family lineage survival is enhanced in high quality landscapes
Insect pollinators such as bumblebees (Bombus spp.) are in global decline1,2, a major cause of which is habitat loss due to agricultural intensification3. A range of global and national initiatives aimed at restoring pollinator habitats and populations have been developed4-6. However, the success of these initiatives depends critically upon understanding how landscape change affects key population-level parameters, such as survival between lifecycle stages7, in target species. Such understanding is lacking for bumblebees because of the difficulty of systematically finding and monitoring colonies in the wild. We used a novel combination of habitat manipulation, land-use and habitat surveys, molecular genetics8 and demographic and spatial modelling to examine between-year survival of family lineages in field populations of three bumblebee species. Here we show that the survival of family lineages from the summer worker to the spring queen stage in the following year increases significantly with the proportion of high-value foraging habitat, including spring floral resources, within 250-1000 m of the natal colony. This is the first evidence of a positive impact of habitat quality on survival and persistence between successive colony cycle stages in bumblebee populations. The findings provide strong support for conservation interventions that increase floral resources at a landscape scale and throughout the season having positive effects on wild pollinators in agricultural landscapes
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