Using Color Infrared Imagery to Detect Sooty Mold and Fungal Pathogens of Glasshouse-propagated Plants

Fungi are major biotic constraints for optimum production and quality of glasshouse plants. When plants are infested with sooty mold (Capnodium spp.) or infected with pathogens, the reflected wavelengths of the electromagnetic spectrum are altered. Spectroradiometric measurements and color infrared (CIR) images of control, honeydew- coated, and sooty mold-infested saplings and individual leaves from trifoliate orange (Poncirus trifoliata), sour orange (Citrus aurantium), ‘Valencia’ orange (C. sinensis), and ‘Bo’ tree (Ficus religiosa) were obtained. Grapefruit saplings and individual leaves infected with Mycosphaerella citri (greasy spot) were imaged under glasshouse conditions. Similarly, muskmelon foliage showing low and high levels of powdery mildew (Sphaerotheca fuliginea) disease severity were analyzed. When examining individual leaves, all fungal biotic stressors generally resulted in variable spectral reflectance data, especially in the blue (450 nm) and green (550 nm) wavelengths; however, values in the red (650 nm) tended to increase and values in the near-IR (850 nm) tended to decrease with stress. Near-IR/red image ratios were significantly reduced (P \u3c 0.05) in stressed whole plant foliage and individual leaves relative to healthy controls. The accumulation of insect honeydew (which occurs before sooty mold infestation) significantly increased (P \u3c 0.05) near-IR reflectance values and near-IR/red ratios in ‘Valencia’ orange and near-IR/ratios in ‘Bo’ tree foliage and individual leaves. Image acquisition and enhancement techniques may prove useful in large-scale production greenhouses where existing infrastructure and high plant populations require high throughput data analysis and identification of biotic stressors

Spectral Reflectance and Digital Image Relations Among Five Aquatic Weeds

This study reports on the use of an artificial quartz halogen lighting source to facilitate the acquisition of spectral light reflectance measurements and digital imaging of invasive aquatic weeds. Spectral leaf or leaf/stem reflectance measurements were made on five aquatic weeds: Eurasian watermilfoil (Myriophyllum spicatum L.), hydrilla [Hydrilla verticillata (L. F.) Royle], parrotfeather [Myriophyllum aquaticum (Vall.), waterhyacinth [Eichhornia crassipes (Mart.) Solms], and waterlettuce (Pistia stratiotes L.). Reflectance measurements were studied at five wavelengths of the electromagnetic spectrum: 450 nm (visible blue), 550 nm (visible green), 650 nm (visible red), 680 nm (visible red edge), and 850 nm (near-infrared). Reflectance values differed significantly (P= 0.05) among the species at all five wavelengths. However, more distinct separations among species occurred at the 550 nm, 650 nm, 680 nm, and 850 nm wavelengths. Reflectance differences among species were attributed to variable foliage coloration and vegetative density. Close range conventional color and color-infrared digital images of leaves or leaves/stems of the five species showed they differed in image tonal response. Reflectance measurements were related to the image tonal response of the plant species on both types of imagery. Supervised image classifications performed on both conventional color and color-infrared images showed the computer generally did an adequate job in identifying the image tonal responses of the weed species

An Acid-Bath Technique to Break Seed Dormancy in Common Sunflower, Helianthus L. annuus (Asteraceae)

The phenomenon of seed dormancy is widespread in plants and serves to prevent all or most of a given population from germinating at the “wrong” time, e.g., during an unusually mild fall in an area subject to typically harsh winters. Seed dormancy is an effective survival strategy in many plant populations, but may greatly complicate efforts to establish large cohorts of seedlings (groups of similar age or developmental stage) needed for re-search and other purposes. In an effort to break seed dormancy in common sunflower, Helianthus annuus L. (Asteraceae), we conducted experiments designed to compare germination times and overall germination success among groups of field-collected H. annuus seeds subjected to several treatments. Overall germination success during a 14-d period posttreatment was lowest among untreated controls and groups soaked in a disinfecting solution for 15-h (1.2% and 2.6% germination, respectively;

An Overview of Aircraft Remote Sensing in Integrated Pest Management

English: This paper presents four exemplary applications of aerial photography and videography, global positioning system (GPS), and geographic information system (GIS) technologies for detecting, monitoring, and mapping insect infestations in agriculture, forestry, and rangeland areas. Applications demonstrated include detecting and mapping: (1) citrus blackfly (Aleurocanthus woglumi Ashby) infestations in citrus orchards; (2) silverleaf whitefly (Bemisia argentifolii Bellows and Perring) infestations in cotton; (3) harvester ant (Pogonomyrmex barbatus F. Smith) infestations on rangelands; and (4) western pine beetle (Dendroctonus brevicomis LeConte) infestations in a forested area. The integration of a GPS with the video imagery permitted latitude and longitude coordinates of insect infestations to be recorded on each image. The GPS coordinates were entered into a GIS to map insect infestations on a regional scale. The integration of remote sensing, GPS, and GIS provide valuable tools that can enable resource managers to develop maps showing the distribution of insect infestations over large areas. The digital imagery can serve as a permanent geographically located image data base for monitoring future contraction or spread of insect infestations over time. Spanish: En este artículo se presentan cuatro ejemplos de las aplicaciones de las tecnologías de fotografía y videografía aéreas, el sistema de posición global (GPS), y del sistema de información geográfica (GIS) para la detección, monitoreo, y mapeo de infestaciones de insectos en áreas agrícolas, forestales y en pastizales. Las aplicaciones demonstradas incluyeron la detección y el mapeo de (1) infestaciones por la mosca negra de los cítricos (Aleurocanthus woglumi Ashby) en huertas de cítricos; (2) mosquita blanca (Bemisia argentifolii Bellows and Perring) en algodón; (3) hormiga cosechadora (Pogonomyrex barbatus F. Smith) en pastizales; y (4) escarabajo del pino del oeste (Dendroctonus brevicomis Le Conte) en una área forestal. La integración del sistema de GPS con las imágenes de video permitió registrar las coordenadas de longitud y latitud en cada imagen. Las coordenadas de cada imagen se incorporaron en un mapa de GIS para crear un mapa de las infestaciones de insectos a una escala regional. La integración de un sistema de detección a distancia, GPS, and GIS brinda herramientas valiosas para permitir a los supervisores de recursos desarrollar mapas que muestren la distribución de infestaciones de insectos en áreas extensas. El sistema de imágenes digital puede servir como una base permanente de datos localizados geográficamente para el monitoreo de futuras concentraciones o dispersiones de plagas de insectos a lo largo del tiempo

Visual Responses of Adult Asian Citrus Psyllid (Hemiptera: Liviidae) to Colored Sticky Traps on Citrus Trees

The effects of five differently-colored sticky traps in capturing adult Diaphorina citri were evaluated in citrus orchards. Trap catches of D. citri were monitored fortnightly on blue, green, red, white and yellow sticky cards placed on three citrus varieties during D. citri active flight period from April to July in south Texas. Evaluation of mean trap catches of each color by repeated measures analysis of variance produced three separate groups: yellow traps caught significantly more D. citri adults than the other four traps; red and green traps caught significantly more D. citri than blue and white traps, which were not significantly different. Although the number of adult psyllid captured on all trap types significantly increased with time during the trapping period, the performance of traps did not change with time. Trap catches were also significantly influenced by the citrus species; traps placed on lemon trees captured more D. citri than those placed on sweet orange and grapefruit, suggesting that plant preference exhibited by D. citri may influence the performance of traps. The ratio of trap reflectance between the 680 to 700 nm and the 450 nm was significantly correlated with total trap catches in all host species studied. Thus, this index was a good indicator of the attractiveness of adult D. citri to colored traps. Additionally, we compared the reflectance values of young versus mature flush shoots of the three host plants used in this study as related to densities of D. citri recorded in colored traps. We discussed the importance of visual cues in the host finding behavior of adult D. citri

Use of Archive Aerial Photography for Monitoring Black Mangrove Populations

A study was conducted on the South Texas Gulf Coast to evaluate archive aerial color-infrared (CIR) photography combined with supervised image analysis techniques to quantify changes in black mangrove [Avicennia germinans (L.) L.] populations over a 26-year period. Archive CIR film from two study sites (sites 1 and 2) was studied. Photographs of site 1 from 1976,1988, and 2002 showed that black mangrove populations made up 16.2%, 21.1%, and 29.4% of the study site, respectively. Photographs of site 2 from 1976 and 2002 showed that black mangrove populations made up 0.4% and 2.7% of the study site, respectively. Over the 26-year period, black mangrove had increases in cover of 77% and 467% on sites 1 and 2, respectively. These results indicate that aerial photographs coupled with image analysis techniques can be useful tools to monitor and quantify black mangrove populations over time

Dune and Vegetation Stability at South Padre Island, Texas, United States of America

Padre Island is the longest of five barrier islands occurring along the Texas Gulf Coast. South Padre Island is separated from the northern two-thirds of the island by the Mansfield Channel. The composition and pattern of vegetation on South Padre Island are relatively well known, but data on the interrelationship of dune and vegetation stability are lacking. We hypothesized that (1) there should be an inverse relationship between elevation change and percent cover on transects across the nearshore dunes of South Padre Island; and (2) percent cover, species composition, and species importance should be most stable where elevation change was least. We tested these hypotheses using three study sites differing in vegetation abundance. Elevation measurements were taken at 1.0-m intervals along three transects at each site using survey-grade Global Positioning System equipment. Vegetation abundance was determined in 10-m intervals along each transect. No sites or topographic zones were devoid of elevation change. Even a site that had a mean percent cover of 65.1% had a mean elevation change of 15.6 cm. However, cover does not have to be great to provide considerable stability. There was no significant difference in elevation change at Site 1, where cover was 57%, and Site 2, where cover was only 12.5%. As hypothesized, there was a significant inverse correlation between elevation change and percent cover when analyzed over all transects and sites, but the relationship did not hold for all sites or topographic zones when these were examined separately. Lack of correlation may be due to differences among sites and zones in the number of different perturbations and their intensities and frequencies. Only Site 2 showed a significant difference in percent cover between the initial and final samples. Species composition and importance were more stable where elevation changes were low

The Biological Flora of Coastal Dunes and Wetlands: Avicennia germinans (L.) L.

Avicennia germinans (L.) L. is a pantropical, subtropical, and occasionally warm-temperate mangrove species that occurs on shorelines that have a broad horizontal tidal range. Also known as black mangrove, stands typically develop under anoxic, water-logged conditions in substrates of silt or clay. Black mangrove can tolerate salinity values ranging from 0 to 90 parts per thousand. Salt is excreted from salt glands on both leaf epidermal surfaces, and aerosol salt spray and salt crystals are frequently observed on the upper leaf epidermis. Avicennia germinans is viviparous. The embryos have no dormancy requirements, and there is no seed bank. This mangrove species has the northernmost distributional range of any mangrove species in North America. It occurs in Louisiana and northern Florida. Color-infrared photography and airborne video imagery techniques have been used successfully to map stands of black mangrove on subtropical coastal shorelines. Reforestation efforts using seedlings and saplings have been successful in several areas of the tropics and subtropics. Avicennia germinans stands play an important role in ecosystem functions as a natural barrier to coastal erosion caused by tropical storms, as habitat for a wide range of organisms in intertidal food chains, and as a carbon repository

Detection of Temporal Changes in Vegetative Cover on South Padre Island, Texas Using Image Classifications Derived from Aerial Color-Infrared Photographs

Supervised image classifications developed from 23 x 23 cm aerial color-infrared aerial photographs (1:5,000 scale) were used to evaluate temporal changes in vegetative cover occurring within three 150 x 300-m research sites on South Padre Island, Texas. Use of high-resolution digitized imagery (ground pixel resolution of ca. 0.1 m) and survey-grade GPS for positional measurements of ground control points (20-25 1.0m2 targets within each research site) resulted in consistently high levels of geometric accuracy, with root mean square errors (RMSEs) ranging between 0.397 – 2.867. Similarly, use of relatively simple information categories (dry and wet sand, live and dead vegetative cover, and water) resulted in supervised image classifications with consistently high levels of overall thematic accuracy (90.0 – 98.0%). Temporal comparisons of image classifications using a cross-tabulation procedure indicated that changes in total vegetative cover had been minimal at most locations during the course the two-year study (2003-2005). However, a localized but significant disruption of native vegetation caused by the dumping of sand by highway maintenance crews was detected in one of the study sites (2004), and ground inspection revealed numerous other sand-dumping sites at various locations on the barrier island. In the first situation, comparison of CIR aerial photographs acquired before and after the sand-dumping incident provided a reliable means by which to evaluate the extent of damage caused by the dumped sand, and to monitor its eventual recovery as a result of recolonization by native plant species