Antibacterial Activity Ods Fractions of Marine Sponge Auletta SP. Against Mycobacterium Smegmatis

The marine sponge collected from tropical coral reefs in Manado North Sulawesi Indonesia was screened for antimicrobial activities. In the screening program to search for antituberculotic inhibitors, the result found that the ethanol extract ODS fractions 4 – 6 with concentration 5 mg/mL of an Indonesian marine sponge Auletta sp. was exhibited inhibitory activity against nonpathogenic Mycobacterium smegmatis at concentration 10, 20, 30µg/disc each fraction respectively. Fractions 4 - 5 with concentration 10µg/disc were most active, the inhibition zone 11, 12 mm, respectively

Antibacterial Screening Activity of Several Sponges Against Staphylococcus aureus, Escherichia coli, Staphylococcus saprophyticus, dan Pseudomonas aeruginosa

Sponge samples collected around Manado waters were obtained 30 species and their crude extracted have been tested in vitro for their activity in inhibiting bacterial growth. Based on the results of antibacterial screening in 30 sponge extracts, there were 23 sponge extracts which had bioactivity in inhibiting the growth of S. aureus, E. coli, S. saprophyticus and P. aeruginosa, Sponge extract No. 43 (of 30 sponge extracts tested) was the most active in inhibiting bacterial growth and had the widest inhibition zone diameter.Keywords: screening, sponge, crude extract, antibacterial, ManadoABSTRAKSampel spons dikoleksi di sekitar Perairan Manado sebanyak 30 jenis/spesies, dimana ekstrak kasarnya telah diuji secara in vitro aktivitasnya dalam menghambat pertumbuhan bakteri. Berdasarkan hasil skrining antibakteri pada 30 ekstrak spons didapatkan hasil ada 23 ekstrak spons yang mempunyai bioaktivitas dalam menghambat pertumbuhan bakteri S. aureus, E. coli, S. saprophyticus dan P. aeruginosa, Ekstrak spons No. 3 (dari 30 ekstrak spons yang diuji) adalah yang paling aktif dalam menghambat pertumbuhan bakteri dan memiliki diameter zona hambat yang paling lebar.Kata kunci: skrining, spons, ekstrak kasar, antibakteri, Manad

Antibacterial Activity Ods Fractions Of Marine Sponge Auletta sp. Against Mycobacterium smegmatis

The marine sponge collected from tropical coral reefs in Manado North Sulawesi Indonesia was screened for antimicrobial activities. In the screening program to search for antituberculotic inhibitors, the result found that the ethanol extract ODS fractions 4 – 6 with concentration 5 mg/mL of an Indonesian marine sponge Auletta sp. was exhibited inhibitory activity against nonpathogenic Mycobacterium smegmatis at concentration 10, 20, 30µg/disc each fraction respectively. Fractions 4 - 5 with concentration 10µg/disc were most active, the inhibition zone 11, 12 mm, respectively.Keywords:  marine sponge, antimicrobial, antituberculosis, Mycobacterium smegmati

Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp

Isolation of marine fungi symbiont of green algae Bornetella sp as a producer of antibacterial compounds has been carried out. This study aims to obtain symbiont fungi from green algae Bornetella sp which produces antibacterial compounds. The symbiont fungus was isolated using the direct planting method. Screening for the antibacterial activity of pure symbiont fungi isolates against Escheriscia coli and Staphylococcus aureus, and was carried out by the agar diffusion method. The isolation results obtained 4 isolates, but the one with the most potential to inhibit the growth of the tested bacteria was MFALM2. Molecular characterization showed that the MFALM2 isolate was identified as Aspergillus nomius with a 100% closeness level. Keywords: isolation; marine fungi; Aspergillus nomius; green alga; Bornetella sp. AbstrakIsolasi jamur laut yang bersimbion dengan alga hijau Bornetella sp sebagai penghasil senyawa antibakteri telah dilakukan. Penelitian ini bertujuan untuk memperoleh jamur simbion dari alga hijau Bornetella sp yang menghasilkan senyawa antibakteri. Isolasi jamur simbion dilakukan dengan metode direct planting. Skrining aktivitas antibakteri isolat murni jamur simbion terhadap bakteri uji Escheriscia coli dan Staphylococcus aureus dilakukan dengan metode difusi agar.  Hasil isolasi diperoleh 4 isolat, namun yang paling berpotensi menghambat pertumbuhan bakteri uji adalah MFALM2. Karakterisasi molekuler menunjukkan bahwa isolat MFALM2 teridentifikasi sebagai Aspergillus nomius dengan tingkat keeratan sebesar 100%.Kata kunci: isolasi; jamur lau; Aspergillus nomius; alga hijau; Bornetella sp

KARAKTERISASI DAN BIOAKTIF ANTIBAKTERI SENYAWA SPONS Haliclona sp. DARI TELUK MANADO

Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai potensi bioaktif yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk menentukan daya hambat antibakteri ekstrak dan fraksi spons Haliclona sp. terhadap bakteri staphylococcus aureus dan Escherichia coli dan menentukan karakteristik senyawa ekstrak dan fraksi spons Haliclona sp yang memiliki daya hambat paling besar. Sampel diekstraksi secara maserasi dan fraksinasi menggunakan pelarut etanol, fraksi heksan, fraksi kloroform dan fraksi metanol. Aktivitas antibakteri dilakukan dengan metode difusi agar (Kirby and Bauer). Ekstrak atau fraksi terbaik dikarakterisasi menggunakan Spektrofotometri UV-Vis, FTIR dan pereaksi Mayer. Hasil penelitian menunjukkan bahwa ekstrak kasar etanol, fraksi kloroform dan fraksi metanol efektif menghambat bakteri Staphylococcus aureus dan fraksi kloroform dan fraksi metanol efektif menghambat bakteri Escherichia coli, ekstrak dan fraksi dikategorikan kuat berdasarkan kriteria Davis dan Stout. Fraksi metanol dengan daya hambat antibakteri paling besar dengan gugus utama N-H, C-H, C-O, C=O diduga mengandung alkaloid yang berpotensi sebagai antibakteri.Kata kunci: Spons Haliclona sp., Aktivitas antibakteri, Staphylococcus aureus, Escherichia col