Performance Analysis and Approximate Message Passing Detection of Orthogonal Time Sequency Multiplexing Modulation

In orthogonal time sequency multiplexing (OTSM) modulation, the information symbols are conveyed in the delay-sequency domain upon exploiting the inverse Walsh Hadamard transform (IWHT). It has been shown that OTSM is capable of attaining a bit error ratio (BER) similar to that of orthogonal time-frequency space (OTFS) modulation at a lower complexity, since the saving of multiplication operations in the IWHT. Hence we provide its BER performance analysis and characterize its detection complexity. We commence by deriving its generalized input-output relationship and its unconditional pairwise error probability (UPEP). Then, its BER upper bound is derived in closed form under both ideal and imperfect channel estimation conditions, which is shown to be tight at moderate to high signal-to-noise ratios (SNRs). Moreover, a novel approximate message passing (AMP) aided OTSM detection framework is proposed. Specifically, to circumvent the high residual BER of the conventional AMP detector, we proposed a vector AMP-based expectation-maximization (VAMP-EM) detector for performing joint data detection and noise variance estimation. The variance auto-tuning algorithm based on the EM algorithm is designed for the VAMP-EM detector to further improve the convergence performance. The simulation results illustrate that the VAMP-EM detector is capable of striking an attractive BER vs. complexity trade-off than the state-of-the-art schemes as well as providing a better convergence. Finally, we propose AMP and VAMP-EM turbo receivers for low-density parity-check (LDPC)-coded OTSM systems. It is demonstrated that our proposed VAMP-EM turbo receiver is capable of providing both BER and convergence performance improvements over the conventional AMP solution.Comment: Accepted in IEEE Transactions on Wireless Communication

Preliminary Assessment of Intramuscular Depot of Lipid-Based Decoquinate Formulation for Long-Term Chemoprophylaxis of Malaria

Sustained-release formulations of decoquinate were evaluated for the long-term prophylaxis of malaria. In the initial experiment, mice were protected from liver-stage Plasmodium infection by intramuscular administration of a lipids-based formulation at a dose of decoquinate 200 mg/kg. The mice that were inoculated with Plasmodium berghei sporozoites 34 days after the administration of a one-time drug dose were continuously monitored for 60 days and shown to be free of Plasmodium parasites. The optimized formulation for the sustained release of decoquinate was prepared by hot melt extrusion, constructed by lipids including cholesterol and mono or diglycerides, and had a drug load of 20 to 40% and particle size of 30 to 50 μm. Decoquinate of the lipids-based formulation was slowly released in vitro at a constant rate for the duration of two months, and was examined and continuously exposed at a therapeutic level in the blood for as long as 4 to 6 months. Further evaluation showed that the lipids-based formulation at doses of decoquinate 100 to 150 mg/kg could protect mice from Plasmodium infection for a period of 120 days. It is the first time that cholesterol has been used for a controlled drug delivery system of decoquinate. The results may provide useful information, not only for preparing a formulation of long-acting decoquinate but also in general for developing a controlled drug release system. The one-time administration of pharmaceutical agents in such a slow-release system may serve patients with no concerns about compliance

Preparation of Monoclonal Antibody for Brevetoxin 1 and Development of Ic-ELISA and Colloidal Gold Strip to Detect Brevetoxin 1

Brevetoxin-1 (BTX-1), a marine toxin mostly produced by the dinoflagellatae Karenia brevis, has caused the death of marine organisms and has had numerous toxicological effects on human health. Hence, it is very necessary to develop a rapid, economical, and reliable immunoassay method for BTX-1 detection. In this study, two kinds of complete antigen were synthesized using the succinic anhydride and isobutyl chloroformate two-step methods. Conjugate BTX-1-OVA was used as an antigen for mice immunization, and BTX-1-BSA for measuring the titer of the produced antibodies. A hybridoma cell line 6C6 stably secreting monoclonal antibody (mAb) against BTX-1 was obtained by fusing SP2/0 myeloma cells with the spleen cells from the immunized mouse. The hybridoma 6C6 was injected into the abdomen of BALB/c mice to obtain ascites, and the anti-BTX-1 mAb was harvested from ascites by precipitation with caprylic acid/ammonium sulfate (CA-AS). The anti-BTX-1 mAb was identified as an IgG1 subtype, and the cross-reactivity results showed that anti-BTX-1 mAb was highly specific to BTX-1 with the affinity of 1.06 × 108 L/mol. The indirect competitive ELISA results indicated that the linear range for BTX-1 detection was 14–263 ng/mL with IC50 of 60 ng/mL, and a detection limit of 14 ng/mL. The average recovery rate from the spiked samples was 88 ± 2% in intra-assay and 89 ± 2% in inter-assay. The limit of detection (LOD) using the colloidal gold strip was 200 ng/mL with high specificity. Therefore, the anti-BTX-1 mAb can be used to detect BTX-1 in shellfish and other related samples

Contrasting responses to drought stress between Chrysanthemum japonense and C. nankingense

The response of Chrysanthemum japonense and C. nankingense to drought stress induced by polyethylene glycol was characterized at the level of leaf water status, leaf surface morphology and cuticular wax (quantity and composition), the activity of antioxidant enzymes, the extent of membrane lipid peroxidation, the accumulation of proline, photosynthesis performance and abscisic acid (ABA) accumulation. The more tolerant species C. japonense maintained its water status more effectively than C. nankingense, probably because its leaves form more cuticular wax and are able to accumulate higher levels of ABA. Superoxide dismutase activity was higher in C. japonense than in C. nankingense, as was that of catalase and ascorbate peroxidase during the later part of the stress episode, but levels of peroxidase were not differentiated at the end of the stress period. Membrane damage, as measured by electrolyte leakage and malondialdehyde accumulation, was less severe in C. japonense, which was also able to generate higher levels of free proline after a 10 h exposure to stress. Thus the superior response of C. japonense also reflects a more adapted system of osmoprotection and antioxidation. As a result, photosynthesis was compromised less by drought stress in C. japonense than in C. nankingense. That provides a scientific basis for the development and application of drought tolerance resources of chrysanthemum

Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior