Pseudomonas Fluorescens and Pseudomonas Putida for Promoting Growth of Jatropha Curcas Seedling Root

Pseudomonas fluorescensandP. putida are Plant Growth Promoting Rhizobacteria (PGPR) that can produce growth hormone. The objective of this study is to know the effects of those two combined species of PGPR on seedling root growth of Jatrophacurcas. The condition of the seedling root determines the success of dry land cultivation. The root which has wider coverage, is larger in number, and is bigger in diameter makes seedling more resistant to stress in dry land environment. In the experiment, two kinds of plant materials are used for seedling, the Jatropha seed and stem material, which are treated in a mixed culture of PGPR. For the Jatropha seed, this mixed culture of PGPR is given at the same time of cultivating the sprout on the seedling medium. For the stem cutting, the PGPR is poured in together during the first watering of the seedling cultivation medium. In the fourthweek, the observed growth parameters are root length, root diameter, primary and secondary lateral root numbers, Root Length Density (RLD), Frequency of Lateral Root (FLR), and Specific Root Length (SRL). These data are analyzed using analysis of variant with DMRT test at 0.05 level of significance. The result of this study shows that PGPR tend to reduce FLR values on the seedling root made from seeds. On the seedling root made from stem cutting, PGPR increase the root length, primary and secondary lateral root numbers, root diameter, FLR and SRL values as well

Keberadaan Bakteri Escherichia Coli Dan Salmonella SP Pada Limbah Pabrik Pakan Unggas (Pakan Ceceran) Yang Difermentasi Dengan Starter Fungsional (the Escherichia Coli and Salmonella SP in Wastes Poultry Feeds (Scattered Feed) That Was Fermented with Fun

The research was aimed to examine the Escherichia coli and Salmonella sp in wastes poultry feeds (scattered feed) that was fermented with fungsional starter (that contain extarct of fermented vegetable waste, rumen fluid, pollard and aquades also called starfungs'). A completely randomined design was applied with 4 treatment and 4 replications. The treatments measured were T0 : scattered feed + 0% ‘starfungs', T1 : scattered feed + 1% ‘starfungs', T2 : scattered feed + 2% ‘starfungs', T3 : scattered feed + 3% ‘starfungs'. The parameters measured were presence Escherichia coli and Salmonella sp in wastes poultry feeds (scattered feed) that was fermented with ‘starfungs'. Analysis of variance and Duncan multiple range test (DMRT) were used to analyze data. The results of research showed that the ‘starfungs' began to level 1% (T1) to suppress the Escherichia coli. Additional ‘starfungs' did not have a significant effect to suppress the Salmonella sp. This could be concluded that ‘starfungs' could suppress the Escherichia coli started from level 1% (T1), however, it could not suppress the Salmonella sp

Kadar Vfa dan Nh3 secara In Vitro Pakan Sapi Potong Berbasis Limbah Pertanian dan Hasil Samping Pertanian Difermentasi dengan A. Niger

The objectives of the research was to determine and assess the effect variation of level and different time periode fermentation cattle feed based agricultural waste on in vitro level of VFA and NH3. Statistic analysis used Completely Randomized Design (CRD) with a 4x4 factorial pattern repeated 3 times to assess the influence of the levels of A. Niger (0, 2, 4 and 6% of dry matter feed) and time periode cattle feed quality (0, 7, 14 and 21 days). The results showed different level starter and time periode fermentation did not show interaction (P<0,05), but each of treatments affect on VFA and NH3 content. Each adding A. Niger and different time periode affect the increased VFA and NH3. Each of adding A. Niger was increased the levels of VFA and NH3 highest in T4 treatment (A. Niger cedar 4%). Each curing time was increase levels of VFA and NH3 highest in treatment B2 (time periode was 14 days)

Pengaruh Penambahan Aras Ekstrak Kubis Sortir Dan Lama Pemeraman Terhadap Kandungan Nutrisi Silase Ikan

This research aim to study the influence of addition of level of cabbage pickle sort to content of nutrisi silase fish. Research executed in two Laboratory that is Technological Laboratory of Food of Livestock. andLaboratory of Science of Livestock Food, Majors of Nutrisi and Livestock Food, Faculty Of Veterinary of University of Diponegoro Semarang. Research Activity by three phase. First phase early making of cabbagecondensation that is by cleaning cabbage beforehand, later;then cut to pieces to minimize and enhanced salt 2,5Yo from fresh weight of cabbage. Afterwards, mingled called a meeting to order anaerob during 4 day tobe taken by its condensation (starter). count of Fish, later;then steamed and extorted obstetrical until aimya lose the. Molasses as much 15% from fresh weight of fish and enhanced by starter of cabbage condensation12,5yo, 15Yo and l7,5Yo from fresh weight of fish runcah. Second phase is the the mixture into stoples and called a meeting to order. treatment of Time during 0, 4, 8 and 12 day. Last phase, silase of fish tested bylaboratoris cover the harsh protein, harsh fat, dusty, harsh fibre and extract substance without nitrogen of according to procedure AOAC (1975). Result of research indicate that treatment of level pickle 12,5, 15 and17,5%o not show the existence of real influence ( p0,05) to harsh protein harsh fat, and harsh fibre.Obstetrical mean silage nutrition fish successively as follows : cruide protein ( 55,32;53,69 and 55,07%); extraceter (3,66;3,64and4,01%); andcruide fibre( 1,07;1,05 and l,l4%). Pursuantto inferentialresearchresult that treatment of addition of level of cabbage pickle sort not influence of quality of silage fis

Pengaruh Pemberian Limbah Pabrik Pakan (Pakan Ceceran) Yang Difermentasi Dengan Starter Fungsional Terhadap Profil Kolesterol Pada Ayam Broiler (the Effect of Waste Poultry Feeds (Scattered Feed) That Was Fermented with Fungsional Starteron Cholesterol Pr

The research was aimed to study the effect of waste poultry feeds (scattered feed) that was fermented with fungsional starter (that contain extarct of fermented vegetable waste, rumen fluid, pollard and aquades also called starfungs) on cholesterol profile in broiler chickens. The material used was 105 day old broiler chick (DOC), waste poultry feeds, commercial feed BR11 and \u27starfungs\u27. A completely randomized design (CRD) was applied with 3 treatments and 7 replications of each treatment. The treatments measured were commercial feed (T0), feed unfermented (T1) and fermented feed (T2). The parameters observed were: total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) on broiler blood. Analysis of variance and Duncan multiple range test (DMRT) were used to analysis data. The results of the research showed that there was no significant effect of feeding fermented waste poultry feeds on blood cholesterol levels, LDL and HDL. The conclusion was that waste poultry feeds fermented with starfungs had no effect on blood cholesterol profile of broiler that could be seen on the level cholesterol, LDL and HDL

Pengaruh Aras Starter Lactobacillus SP Terhadap Performa Mikrobiologi Silase Ikan Dilihat Dari Total Bakteri, Bakteri Asam Laktat Dan Fungi

The aim of the research was to evaluate the effect of addition starter Lactobacillus sp on microbiologic performances was been seen from total bacterial, lactic acid bacteria and fungal of fish iilage. The researih material were fish waste (ikan rucah), rice bran, Lactobacillus sp, fisiologic NaCl, NA (Nutrient Agar), MRS (de Man, Rogosa, and Sharpe) Broth, and SGA (Saboroud Glucose Agar). The completely randomized design was been used on this research with 5 treaments and 3 replications. The treatments were C0 (level starter 07o), Cl (level starter2Yo), C2 (level starter 4o/o), C3 (level starter 60/o) and C4 (level starter 8%). fheparameters were total bacterial, lactic acid bacteria and fungal of fish silage. The results showed that addition of starter Lactobacillus sp has no affected to total bacterial and fungal of fish silage. The addition increased(p<0,01) total lactic acid bacteria of fish silage. The conclusion was addition of starter Lactobacillus rp wir been stored during 2 weeks no affect total bacteria and fungi of fish silage. Addition of 4% level itarterLactobacillus sp increased total lactic acid bacteria offish silage.Keyword ; stafter Lactobacillus sp, fish silage, total bacteria, total fung

Isolasi Dan Identifikasi Bakteri Asam Laktat Pada Caecum Ayam Daging (Isolation and Ldentitication of Lactic Acid Bacteria in the Broiler's Caecum)

Lactic acid bacteria is one of natural microbe which is often used as fermentation agent. The Purpose of this researchwas isolated and identified lactic acid bacteria in broiler's caecum. Exploration experimental method by 6 repetition with 2 sub research was used. The ldentification of Lactic acid bacteria was based on morphological, biochemical and physiological of the character. The morphological characteristic were shape cell and gram test. The biochemical characteristic was catalase test. The physiological characteristic was clear zone test. The result obtained l2 isolates of Lactic acid bacteria, rod and coccus , gam positive, catalase negative and showed clear zone colony at lok CaCOt in MRS. That were early characteristic of lactic acid bacteria which were apparenly as genus of Lactobacillus and Leuconostoc

ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PADA CAECUM AYAM DAGING (Isolation and ldentitication of Lactic Acid Bacteria in the Broiler's Caecum)

Abstract Lactic acid bacteria is one of natural microbe which is often used as fermentation agent. The Purpose of this researchwas isolated and identified lactic acid bacteria in broiler's caecum. Exploration experimental method by 6 repetition with 2 sub research was used. The ldentification of Lactic acid bacteria was based on morphological, biochemical and physiological of the character. The morphological characteristic were shape cell and gram test. The biochemical characteristic was catalase test. The physiological characteristic was clear zone test. The result obtained l2 isolates of Lactic acid bacteria, rod and coccus , gam positive, catalase negative and showed clear zone colony at lok CaCOt in MRS. That were early characteristic of lactic acid bacteria which were apparenly as genus of Lactobacillus and Leuconostoc. Keywords : caecum, Lactic acid bacteri

Meningkatkan Kemampuan Bekerjasama melalui Bermain Simbolik

The problem of this research was whether or not students' cooperative skill can be improved through symbolic games. The aim of this research was to improve students' cooperative skill through symbolic games. The type of this research was a classroom action research consisted of two cycles with three meetings in every cycle. The subject of this research was B2 group of PAUD (early childhood education program) of Haqiqi Bengkulu city with the total of 10 students. The technique of collecting the data was done by doing observation. The data analysis was conducted by using mean test. The result of this research showed that symbolic games activity can improve the students' cooperative skill. The mean score for students' cooperative skill was 4.435 or in good criteria. Therefore, it is recommended for the teacher that symbolic games can be applied to improve the students' cooperative skill