Distribution of BoLA-DRB3

The role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in the BoLA-DRB3 in Iranian buffaloes. Heminested PCR-RFLP method was used to identify the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the study herd (12 alleles). Almost 63.50% of the alleles were accounted for by four alleles (BoLA-DRB3.2 *48, *20, *21, and obe) in Iranian buffalo. The DRB3.2 *48 allele frequency (24.20%) was higher than the others. The frequencies of the DRB3.2 *20 and DRB3.2 *21 are 14.52 and 14.00, respectively, and obe and gbb have a new pattern. Significant distinctions have been found between Iranian buffalo and other cattle breed studied. In the Iranian buffaloes studied alleles associated with resistance to various diseases are found

Annual dynamics of parameters of physical development of boys and girls aged 18 and 19

The questions concerning the terms of juvenile age body measurement as well as body composition variability remain relevance and academic novelty. Such studies results data are essential for systematic analysis on secular trend of young generation development.Material and methods. Longitudinal study of physical development (length, body weight, muscle and fat component) of boys and girls aged 18 and 19, studying at 1–2 courses of university and living in a dormitory has been applied.Results and discussion. In boys and girls aged 18–19, body length and body weight have increased for 1 year on average by 1.5 and 0.9 cm and by 1.3 and 1.2 kg, respectively. In 22.6 % of boys and 54.8 % of girls body length does not change. The annual changes in the fat component are not reliable, whereas the muscle component increases significantly by 3 % in boys and by 0.8 % in girls. Students living in a dormitory often experience a lack of night sleep, they do not have enough physical activity, consume not enough calories, valuable proteins and fats. Because of this, physical development may deteriorate.Conclusions. The annual variability of the indicators of physical development of students aged 18–19 years indicates the ongoing processes of growth and development, which are more pronounced in boys, in girls there is a tendency to stabilize the length of the body. Knowledge about the sensitivity of the body of first-year students mastering the requirements for studying at a university and independent living in a dormitory is necessary for the organization of work on pedagogical support during the adaptation of first-year students

Clustering as a criterion for the success of modern industrial enterprises

The paper reveals the problems of the use of clusters as an important factor of innovative development of Russian enterprises and territorial entities delineated a theoretical basis for the existence of territorial formations, showing their relationship with the clusters displayed in the cluster's functioning as an open system. All the more important in the formation of innovation territorial entities in Russia acquire the cluster structure based on cooperation of enterprises, financial institutions, educational institutions etc. In the developed countries have long had and continue to have such structures in various industries. In the works of famous foreign scientists clearly defined theoretical bases of formation and functioning of clusters, use cluster models to ensure the competitiveness of the economy, their advantages and disadvantages. Foreign experience can be useful to develop a national strategy for clustering of Russia, with a comprehensive study of its socioeconomic development. These tasks at this stage be a priority for Central and local public authorities. Issues related to the principles of clusters, innovation economy, have been studied by many scientists, both Russian and foreign. In published works, the essence of cluster analysis, given the definition of "cluster" classification of clusters, the role of innovative development of economy, experience of the use of cluster models, etc

Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library

A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-β-lactamase: casein proteins synthesized in Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine χ-casein, clone 3-H7 contained a cDNA fragment of β-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of α_si-casein

Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library

A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-β-lactamase: casein proteins synthesized in Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine χ-casein, clone 3-H7 contained a cDNA fragment of β-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of α_si-casein

Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences

BACKGROUND: In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences. METHODS: Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLASTN program and public databases. To localise the INTs PCR-based screening of GeneBridge4-RH-panel was used. RESULTS: Twelve cellular sequences adjacent to integrated HPV16 (INT markers) expressed in squamous cell cervical carcinomas were isolated. For 11 INT markers homologous human genomic sequences were readily identified and 9 of these showed significant homologies to known genes/ESTs. Using the known locations of homologous cDNAs and the RH-mapping techniques, mapping studies showed that the INTs are distributed among different human chromosomes for each tumour sample and are located in regions with the high levels of expression. CONCLUSIONS: Integration of HPV genomes occurs into the different human chromosomes but into regions that contain highly transcribed genes. One interpretation of these studies is that integration of HPV occurs into decondensed regions, which are more accessible for integration of foreign DNA

A Genome Scan for Positive Selection in Thoroughbred Horses

Thoroughbred horses have been selected for exceptional racing performance resulting in system-wide structural and functional adaptations contributing to elite athletic phenotypes. Because selection has been recent and intense in a closed population that stems from a small number of founder animals Thoroughbreds represent a unique population within which to identify genomic contributions to exercise-related traits. Employing a population genetics-based hitchhiking mapping approach we performed a genome scan using 394 autosomal and X chromosome microsatellite loci and identified positively selected loci in the extreme tail-ends of the empirical distributions for (1) deviations from expected heterozygosity (Ewens-Watterson test) in Thoroughbred (n = 112) and (2) global differentiation among four geographically diverse horse populations (FST). We found positively selected genomic regions in Thoroughbred enriched for phosphoinositide-mediated signalling (3.2-fold enrichment; P<0.01), insulin receptor signalling (5.0-fold enrichment; P<0.01) and lipid transport (2.2-fold enrichment; P<0.05) genes. We found a significant overrepresentation of sarcoglycan complex (11.1-fold enrichment; P<0.05) and focal adhesion pathway (1.9-fold enrichment; P<0.01) genes highlighting the role for muscle strength and integrity in the Thoroughbred athletic phenotype. We report for the first time candidate athletic-performance genes within regions targeted by selection in Thoroughbred horses that are principally responsible for fatty acid oxidation, increased insulin sensitivity and muscle strength: ACSS1 (acyl-CoA synthetase short-chain family member 1), ACTA1 (actin, alpha 1, skeletal muscle), ACTN2 (actinin, alpha 2), ADHFE1 (alcohol dehydrogenase, iron containing, 1), MTFR1 (mitochondrial fission regulator 1), PDK4 (pyruvate dehydrogenase kinase, isozyme 4) and TNC (tenascin C). Understanding the genetic basis for exercise adaptation will be crucial for the identification of genes within the complex molecular networks underlying obesity and its consequential pathologies, such as type 2 diabetes. Therefore, we propose Thoroughbred as a novel in vivo large animal model for understanding molecular protection against metabolic disease

Clustering as a criterion for the success of modern industrial enterprises

The paper reveals the problems of the use of clusters as an important factor of innovative development of Russian enterprises and territorial entities delineated a theoretical basis for the existence of territorial formations, showing their relationship with the clusters displayed in the cluster's functioning as an open system. All the more important in the formation of innovation territorial entities in Russia acquire the cluster structure based on cooperation of enterprises, financial institutions, educational institutions etc. In the developed countries have long had and continue to have such structures in various industries. In the works of famous foreign scientists clearly defined theoretical bases of formation and functioning of clusters, use cluster models to ensure the competitiveness of the economy, their advantages and disadvantages. Foreign experience can be useful to develop a national strategy for clustering of Russia, with a comprehensive study of its socioeconomic development. These tasks at this stage be a priority for Central and local public authorities. Issues related to the principles of clusters, innovation economy, have been studied by many scientists, both Russian and foreign. In published works, the essence of cluster analysis, given the definition of "cluster" classification of clusters, the role of innovative development of economy, experience of the use of cluster models, etc

The cientificWorldJOURNAL Research Article Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

The role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in the BoLA-DRB3 in Iranian buffaloes. Heminested PCR-RFLP method was used to identify the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the study herd (12 alleles). Almost 63.50% of the alleles were accounted for by four alleles (BoLA-DRB3.2 * 48