Large-scale phenotyping of patients with long COVID post-hospitalization reveals mechanistic subtypes of disease

One in ten severe acute respiratory syndrome coronavirus 2 infections result in prolonged symptoms termed long coronavirus disease (COVID), yet disease phenotypes and mechanisms are poorly understood1. Here we profiled 368 plasma proteins in 657 participants ≥3 months following hospitalization. Of these, 426 had at least one long COVID symptom and 233 had fully recovered. Elevated markers of myeloid inflammation and complement activation were associated with long COVID. IL-1R2, MATN2 and COLEC12 were associated with cardiorespiratory symptoms, fatigue and anxiety/depression; MATN2, CSF3 and C1QA were elevated in gastrointestinal symptoms and C1QA was elevated in cognitive impairment. Additional markers of alterations in nerve tissue repair (SPON-1 and NFASC) were elevated in those with cognitive impairment and SCG3, suggestive of brain–gut axis disturbance, was elevated in gastrointestinal symptoms. Severe acute respiratory syndrome coronavirus 2-specific immunoglobulin G (IgG) was persistently elevated in some individuals with long COVID, but virus was not detected in sputum. Analysis of inflammatory markers in nasal fluids showed no association with symptoms. Our study aimed to understand inflammatory processes that underlie long COVID and was not designed for biomarker discovery. Our findings suggest that specific inflammatory pathways related to tissue damage are implicated in subtypes of long COVID, which might be targeted in future therapeutic trials

SARS-CoV-2-specific nasal IgA wanes 9 months after hospitalisation with COVID-19 and is not induced by subsequent vaccination

BACKGROUND: Most studies of immunity to SARS-CoV-2 focus on circulating antibody, giving limited insights into mucosal defences that prevent viral replication and onward transmission. We studied nasal and plasma antibody responses one year after hospitalisation for COVID-19, including a period when SARS-CoV-2 vaccination was introduced. METHODS: In this follow up study, plasma and nasosorption samples were prospectively collected from 446 adults hospitalised for COVID-19 between February 2020 and March 2021 via the ISARIC4C and PHOSP-COVID consortia. IgA and IgG responses to NP and S of ancestral SARS-CoV-2, Delta and Omicron (BA.1) variants were measured by electrochemiluminescence and compared with plasma neutralisation data. FINDINGS: Strong and consistent nasal anti-NP and anti-S IgA responses were demonstrated, which remained elevated for nine months (p < 0.0001). Nasal and plasma anti-S IgG remained elevated for at least 12 months (p < 0.0001) with plasma neutralising titres that were raised against all variants compared to controls (p < 0.0001). Of 323 with complete data, 307 were vaccinated between 6 and 12 months; coinciding with rises in nasal and plasma IgA and IgG anti-S titres for all SARS-CoV-2 variants, although the change in nasal IgA was minimal (1.46-fold change after 10 months, p = 0.011) and the median remained below the positive threshold determined by pre-pandemic controls. Samples 12 months after admission showed no association between nasal IgA and plasma IgG anti-S responses (R = 0.05, p = 0.18), indicating that nasal IgA responses are distinct from those in plasma and minimally boosted by vaccination. INTERPRETATION: The decline in nasal IgA responses 9 months after infection and minimal impact of subsequent vaccination may explain the lack of long-lasting nasal defence against reinfection and the limited effects of vaccination on transmission. These findings highlight the need to develop vaccines that enhance nasal immunity. FUNDING: This study has been supported by ISARIC4C and PHOSP-COVID consortia. ISARIC4C is supported by grants from the National Institute for Health and Care Research and the Medical Research Council. Liverpool Experimental Cancer Medicine Centre provided infrastructure support for this research. The PHOSP-COVD study is jointly funded by UK Research and Innovation and National Institute of Health and Care Research. The funders were not involved in the study design, interpretation of data or the writing of this manuscript

Productivity of three sub-types of Sudan desert sheep at El Huda research station, Sudan

Shugor, Dubasi and Watish sub-types of Sudan Desert sheep were evaluated on the basis of 3 productivity indices (weight of lamb weaned/ ewe/yr, weight weaned/kg ewe/yr and weight weaned/kg 0.75/ewe/yr). Components used to construct the indices were litter weight at 120 d, parturition interval, survival to 120 d and ewe postpartum weight. There were differences among sub-types in all components but, because of interactions among them, there were no differences in the indices. The indices were 16.8 kg, 419 g and 1.14 kg and were lower than those found in other studies. Suggestions for improving productivity are made

GROWTH AND YIELD OF BEETROOT (BETA VULGARIS) AS AFFECTED BY THE APPLICATION OF DIFFERENT SOURCES AND VARYING RATES OF NUTRIENTS OF ANIMAL ORIGIN IN GIDAN KWANU, MINNA, NIGER STATE OF NIGERIA

Two experiments were conducted concurrently at the Horticultural Nursery of the Department of Crop Production, Federal University of Technology. Gidan Kwanu, Minna (latitude 6o 30’ N and Longitude 90 40’ E) during the dry season of 2016. The objective of the two trials was to determine the nutrient source and rates required in the production of beetroot (Beta vulgaris). The experimental treatment consists of three organic nutrient sources namely; cow dung, poultry manure and goat droppings at ratio by weight of 1:1:2 and 1:1:4 of sand: topsoil and organic nutrient. The experiments were laid arranged in completely randomized design.(CRD) and replicated thrice. Improved seeds of beetroot were soaked for 24 hours and sown at 3cm depth. Data were collected on growth parameters such as crop germination, crop vigor, plant height, number of offshoot, leaf area index, shoot girth, and fresh leafy yield. All data collected were subjected to analysis of variance (ANOVA) and the means were separated using Duncan multiple range test(DMRT). Result obtained indicated that 10t ha-1 poultry manure exhibited better growth performance of beetroot and resulted in higher fresh leafy yield than all other organic sources and rates. The study therefore recommends the application of poultry manure at the rate of 10 t ha-1 for beetroot farmers in the study area