Transcriptional analysis of highly syntenic regions between Medicago truncatula and Glycine max using tiling microarrays

The comparative transcriptional analysis of highly syntenic regions in six different organ types between Medicago truncatula (barrel medic) and Glycine max (soybean), using nucleotide tiling microarrays, provides insights into genome organization and transcriptional regulation in these legume plants

PIF3 Is Involved in the Primary Root Growth Inhibition of Arabidopsis Induced by Nitric Oxide in the Light

PHYTOCHROME INTERACTING FACTOR3 (PIF3) is an important component in the phytochrome signaling pathway and mediates plant responses to various environmental conditions. We found that PIF3 is involved in the inhibition of root growth of Arabidopsis thaliana seedlings induced by nitric oxide (NO) in light. Overexpression of PIF3 partially alleviated the inhibitory effect of NO on root growth, whereas the pif3-1 mutant displayed enhanced sensitivity to NO in terms of root growth. During phytochrome signaling, the photoreceptor PHYB mediates the degradation of PIF3. We found that the phyB-9 mutant had a similar phenotype to that of PIF3ox in terms of responsiveness to NO. Furthermore, NO treatment promoted the accumulation of PHYB, and thus reduced PIF3 content. Our results further show that the activity of PIF3 is regulated by the DELLA protein RGL3[RGA (repressor of ga1-3) LIKE 3]. Therefore, we speculate that PIF3 lies downstream of PHYB and RGL3, and plays an important role in the inhibitory effect of NO on root growth of Arabidopsis seedlings in light

Classification of TARs based on physical location relative to the predicted genes

Pie charts showing percentage of all identified TARs in different genome components relative to the predicted gene structures in barrel medic and soybean. Number of non-exonic TARs in different sub-genic regions in barrel medic and soybean.<p><b>Copyright information:</b></p><p>Taken from "Transcriptional analysis of highly syntenic regions between and using tiling microarrays"</p><p>http://genomebiology.com/2008/9/3/R57</p><p>Genome Biology 2008;9(3):R57-R57.</p><p>Published online 19 Mar 2008</p><p>PMCID:PMC2397509.</p><p></p

Tiling microarray detection of the predicted genes in the 1 Mb region syntenic between barrel medic and soybean

Pie charts showing the number and percentage of genes detected by tiling arrays in at least one of the six examined organ types. Tiling array detection rates of predicted genes in the six organ types in barrel medic and soybean.<p><b>Copyright information:</b></p><p>Taken from "Transcriptional analysis of highly syntenic regions between and using tiling microarrays"</p><p>http://genomebiology.com/2008/9/3/R57</p><p>Genome Biology 2008;9(3):R57-R57.</p><p>Published online 19 Mar 2008</p><p>PMCID:PMC2397509.</p><p></p