Karakterisasi Senyawa Aktif Antioksidan Dan Antibakteri Dalam Ekstrak Etanol Buah Namnam (Cynometra Cauliflora L.)

Telah dilaporkan penelitian untuk mengetahui senyawa yang memiliki aktivitas antioksidan dan antibakteri dalam ekstrak etanol buah namnam (C. cauliflora L.) menggunakan instrumen GC-MS. Hasil analisa GC-MS menunjukkan adanya senyawa 5-hiroksimetilfurfural sebagai komponen utama dalam ekstrak etanol buah namnam. Ekstrak etanol buah namnam memiliki aktivitas antioksidan dengan nilai IC50 sebesar 328,29 ppm dan memiliki aktivitas antibakteri terhadap E. coli dan S. aureus dengan zona hambat masing-masing 16 mm pada konsentrasi 20%

Karakt£risasi Senyawa Aktif Anti Bakteri Minyak Atsiri Bunga Cengkeh (Syzygium Arimaticum)

Water distillation and purification of drought clove (Syzygium arimaticum) interest yields clove interest essential oils 6,5 % (v/w), rust colored ofchocolate, refractive index (25°C) 1,52874, specific mass (25°C) 1,0636 g/ml and condensation in ethanol 70% (1:2) limpid. Oil of clove haves the character of antibacterial to five test bacteriums ( B. subtilis, B. cereus, S. aureus, P. aeroginosa and E. coli). Result of analysis GCMS shows existence of eugenol compound (72,98%), kariaphilena (10,40%), a-humulena (1,04%) and acetate eugenol ( 15,58%). SpectraphotometerJKTI, VOL. 12, No.1, Juni 2010 analysis UV- Vis existence of absorption at Amaks 207,63 nm (ethanol) and 283,76 nm (eugenol). Analysis FTIR existence of bunch - OH (3543,39 em'), aliphatic C-H (1605,79 - 1766,90 em'), aromatic C=C ( 1433,60 - 1514,74 em'), C-Caril (1433,60-1514,74em-I) and cO (1268,44 em')

Uji Toksisitas Ekstrak Daun Pandan Wangi (Pandanus Amaryllifolius Roxb.) Dengan Metode Brine Shrimp Lethality Test (BSLT)

Telah dilakukan penelitian untuk mengetahui toksisitas dari ekstrak daun pandan wangimenggunakan metode Brine Shrimp Lethality Test (BSLT). Ekstrak dibuat dengan cara maserasimenggunakan tiga macam pelarut, yaitu butanol, etil asetat, dan petroleum eter. Uji toksisitasdilakukan dengan menggunakan larva udang Artemia salina Leach yang berumur 48 jam. Efektoksik masing-masing ekstrak diidentifikasi dengan presentase kematian larva udangmenggunakan analisis probit (LC50). Ekstrak aktif kemudian diuji kandungan fitokimianya dansenyawa bioaktif yang terkandung di dalamnya dengan menggunakan GC-MS. Hasilnyamenunjukkan ekstrak etil asetat bersifat toksik (LC50 : 288,4 ppm). Senyawa yang terkandungdalam ekstrak etil asetat adalah senyawa terpenoid dan steroid

Uji Potensi Aktivitas Anti Kanker Ekstrak Daun Pandan Wangi (Pandanus Amaryllifolius Roxb.) Dengan Metode Brine Shrimp Lethality Test (Bslt)

Has been done research to know anticancerpotencial activity from fragrant screw pine leaf extractapplies method Brine Shrimp Lethality Test ( BSLT).Extract is made by the way of macerate to apply threekinds of solvent, that is butanol, ethyl acetate, and etherpetroleum. Toxicity test is done by using prawn larvaArtemia salina Leach which age 48 hours. Toxic effecteach extract is identified with presentase death of prawnlarva applies probit analysis (LC50). Active extract thenis tested its the phytochemistry content and compoundbioa1(fi! suggested applies GC-MS. Result of his (itsshowing ethyl acetate extract to have the character oftoxic ( LC50 : 288,4 ppm). The toxic compounds whichpredietion.implied in ethyl acetate extract is terpenoidsand steroid

Sifat Fisiko Kimia Dan Aktivitas Antioksidan Minyak Kelapa Murni (Vco) Hasil Fermentasi Rhizopus Orizae

Research on physicochemical properties and antioxidant activity of VCO obtained from Rhizopus orizaefermentation had been done. Antioxidant activity was determined by DPPH method. Production of VCO had been carried out from coconut sample obtained from Pandeglang region with fermentation by Rhizopusorizae in variation of inoculum asfollow 2%, 5%, 7%, 10% and 12 %. Physicochemical test consist of density, refractive index, water content, free fatty acid, iodine value, peroxide value while the composition of fatty acid determined by GCMS, The result showed that variation of inoculum had significantly 0,024 influenced the yielded of VCO producted. Based on this result, quality of VCO still in the range of CODEX Standard, 19-1991rev.2-1999. It was found that antioxidant activity of VCO only 6.3% at 1000 ppm. The composition offatty acid based in lauric acid content measured by GCMS showed a significant result (49.48%-50%) agreed with COD EX Standard

Karakterisasi Senyawa Hasil Isolasi Dari Ekstrak Etil Asetat Daun Namnam (Cynometra Cauliflora L.) Yang Memiliki Aktivitas Antibakteri

Namnam is known as a plant family of fabacea that contains phenolic compounds with diverse biological activities including antibacterial. The aim of this study was to isolate and characterize the antibacterial activity of compounds isolated from ethyl acetate extract of the leaves namanam (Cynometra cauliflora L.). Extraction was done by maceration, fractionation by column chromatography, antibacterial essay by disc diffusion method, and characterization of compounds with UV-Vis, FTIR spectroscopy and LCMS. Ethyl acetate extracts have antibacterial activity with inhibition zone respectively 12.25 and 6.00 mm to Escherecia coli and Stapilococcus aureus at a concentration of 100,000 ppm. The results of column chromatography fractions 23-30 yielded three isolates with Rf 0.20 cm; 0.33 cm and 0.87 cm. Characteristics of antibacterial active compounds in isolates 2 (Rf 0.33 cm) is based on the analysis results are UV-Vis absorption at λmaks 206.93 nm, 268.40 nm, 328.58 nm, 383.98 nm and 386.98 nm , FTIR (KBr) showed -OH group 3415.68 cm-1, CH 2958.10 cm-1, C = C aromatic 1651.18 cm-1, C-OH cyclic 1019.88 cm-1 and CH aromatic 694.56 cm-1 and LCMS produce three main peak at a retention time of 4.82; 6.87 and 7.64 which is thought to be the compound 2-isopropyl-5-metilsikloheksil 2-hidroksipropanoat, Cuelure, and 2-[(2-Hydroxycyclohexyl) oxy] cyclohexanecarboxylate

Elusidasi Struktur Kimia Senyawa Bioaktif Pengendali Serangga Ulat Kubis Dari Kulit Batang Aglaia Disoxylum (Meliaceae)

Telah dilakukan penelitian elusidasi struktur kimia senyawa fenol-2-(1-metiletoksi)-metilkarbamat, yang bersifat bioaktif (LC50 = 3,57 ppm) pengendali serangga ulat kubis(Crocidolomia binotalis Zeller) dari ekstrak etil asetat kulit batang Aglaia disoxylum(Meliaceae), salah satu tumbuhan yang dikenal sebagai pasak bumis. Senyawa inidiperoleh berupa kristal tidak berwarna, berbentuk jarum (40 mg), titik leleh 99–101 oC,rumus molekul C11H15NO3 dan BM = 209. Penetapan struktur kimia senyawa tersebutdilakukan berdasarkan data spektroskopi UV-VIS, FTIR, 1H dan 13C NMR, HMQC,HMBC, dan MS (GC-MS)

Aktivitas Antidiabetes Ektrak Etil Asetat Daun Pandan Wangi (Pandanus Amaryllifolius Roxb.)

This research done to know activity antidiabetes from fragrant screw pine leaf ethyl acetate extract (Pandanus amaryllifolius Roxb.) applies method aglukosidase. Extract is made by the way ofmaceration to apply ethyl acetate. Test antidiabetes is done by using enzyme a-glukosidase and PNP-a-D-glukopiranosida. Fragrant screw pine leaf ethyl acetate extract haves the character of antidiabetes with resistance activity, IC50 value as 94,23 ppm. Result of GCMS identification shows fragrant screw pine leaf ethyl acetate extract contains active compound of its the fatty acid and deriuaies, terpenoids, and steroid

Uji Aktivitas Antibakteri Ekstrak Air Bunga Kecombrang terhadap Bakteri E. Coli dan S. Aureus sebagai Bahan Pangan Fungsional

The testing has been conducted research on the antibacterial activity of aqueous extracts of flowers kecombrang against E. coli and S. Aureus. This study was aims to provided scientific evidence of excellence kecombrang plants as functional food ingredients. Antibacterial activity assays performed using dispersive method. From the results of antibacterial testing kecombrang flower water extract against E. coli concentration of 20% = 0 mm, 40% = 0 mm, 60% = 4.8 mm, 80% = 5.2 mm, 100% = 7.3 mm and the test bacteria S. aureus concentration of 20% = 8.67 mm, 40% = 9.11 mm, 60% = 12:33 mm, 80% = 12:44 mm, 100% = 13.89 mm