2 research outputs found
High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP markers
Background: Genetic diversity and genetic variation of 10 populations
and subpopulations of Magnolia wufengensis , a new and endangered
endemic species, were examined by inter simple sequence repeat (ISSR)
and sequence-related amplified polymorphism (SRAP) molecular markers.
Compared with other endangered endemic Magnolia taxa, M. wufengensis
holds a relatively high level of genetic variation. Result: Total
genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP
markers. For polymorphic loci (P), the effective mean number of alleles
(Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the
mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for
SRAP markers. Within-population variation was estimated for P as 74.9%
using ISSR and 74.6% with SRAP markers; the number of alleles Ae was
1.379 with ISSR and 1.397 for SRAP and H 0.235 with ISSR and 0.247 for
SRAP markers. Conclusion: The analysis of molecular variation of both
ISSR and SRAP marker systems indicated that most genetic variation is
within populations,with values of 90.64% and 82.92% respectively.
Mantel tests indicated a moderate association between the two marker
systems and a low correlation between genetic and geographic distances.
High levels of genetic diversity and low levels of population
divergence suggest that genetic drift is not currently of great concern
for this species. Severe habitat loss and fragmentation, predominantly
ascribed to anthropogenic pressures, caused in-situ developing
restriction of this species. Action for conserving this rare species
for its long-term survival should be taken immediately
High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP markers
Background: Genetic diversity and genetic variation of 10 populations and subpopulations of Magnolia wufengensis, a new and endangered endemic species, were examined by inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Compared with other endangered endemic Magnolia taxa, M. wufengensis holds a relatively high level of genetic variation.
Result: Total genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP markers. For polymorphic loci (P), the effective mean number of alleles (Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for SRAP markers. Within-population variation was estimated for P as 74.9% using ISSR and 74.6% with SRAP markers; the number of alleles Ae was 1.379 with ISSR and 1.397 for SRAP and H 0.235 with ISSR and 0.247 for SRAP markers.
Conclusion: The analysis of molecular variation of both ISSR and SRAP marker systems indicated that most genetic variation is within populations, with values of 90.64% and 82.92% respectively. Mantel tests indicated a moderate association between the two marker systems and a low correlation between genetic and geographic distances. High levels of genetic diversity and low levels of population divergence suggest that genetic drift is not currently of great concern for this species. Severe habitat loss and fragmentation, predominantly ascribed to anthropogenic pressures, caused in-situ developing restriction of this species. Action for conserving this rare species for its long-term survival should be taken immediately