Crescimento e sobrevivência da ostra de fundo, Crassostrea gasar, cultivada no Nordeste e Sul do Brasil.

This study evaluated the growth, survival, and time to reach the minimum market size (50 mm shell height) of the bottom oyster Crassostrea gasar with seeds produced at hatchery. Culture areas were located in the States of Maranhão [1-Morro do Meio (MM); 2-Torto (TT)] and Santa Catarina [1-São Francisco do Sul (SFS); 2-Florianópolis (SB)]. Eight thousand seeds were transferred to each location and cultivated from June 2012 to July 2013. Oyster growth in Santa Catarina was bigger than in Maranhão..

Optimizing broodstock conditioning for the tropical clam Anomalocardia brasiliana

The tropical clam Anomalocardia brasiliana is a commercially important bivalve living along the south Atlantic coast of Latin America. Harvest of natural stocks is the principal method for supplying market demand-the market average price ranging from 5 to 10 US$ per kilogram of meat. As a first step in the development of hatchery techniques for A. brasiliana seed production, this study examined the effects of temperature and salinity on conditioning broodstock for spawning. Two treatments tested conditioning at 25 degrees C at salinities of either 30 or 35. A third treatment examined the effects of an initial 10-day conditioning at 16 degrees C followed by a gradual temperature increase to 25 degrees C at a salinity of 35. As a control, clams were sampled from the wild over the same experimental period. Tissue samples were taken at the initiation of the experiment and at 15, 30, 45, and 55 days of conditioning and examined histologically to determine changes in the sexual development of the clams. Four reproductive stages were identified during the experimental period: gametogenesis, mature, spawned, and absorption. The treatment with the initial 10-day conditioning period at 16 degrees C demonstrated that it is possible to synchronize gamete development in both males and females to result in the maximum proportion of mature broodstock at 55 days of conditioning

Anesthesia in oysters of the genus crassostrea cultured in Brazil.

201

Assessing in vitro combinations of antifungal drugs against yeasts and filamentous fungi: comparison of different drug interaction models.

Contains fulltext : 48405.pdf (publisher's version ) (Closed access)Non-parametric and parametric approaches of two competing zero-interaction theories--the Loewe additivity and the Bliss independence - were evaluated for analyzing the in vitro interactions of various antifungal drugs. Fifty-one data sets, derived from three drug combinations, tested in triplicate against 17 clinical yeast and mold isolates with a two-dimensional checkerboard microdilution technique, were selected to span from strong synergy to strong antagonism. These were analyzed with the standard FIC index model and modern concentration-effect response surface models: the fully parametric model developed by Greco et al. and the 3-D analysis developed by Prichard et al. The FIC index model is subjective, sensitive to experimental errors and resulted in approximated results and variable conclusions depending on the MIC endpoints determined and interpretation endpoints used. By using the MIC-2 endpoint (lowest drug concentration showing 50% of growth) for calculating the FIC indices, problems due to trailing phenomena were reduced and weak interactions could be detected; higher levels of reproducibility and agreement with the other models were achieved using the MIC-0 and MIC-1 (lowest drug concentration showing 10 and 25% of growth, respectively). High reproducibility was achieved in interpreting the FIC indices when the cutoffs of 0.25 and 4 (for single experiments) and the cutoff of 1 (for replicates) were used for defining the limits of additivity/indifference. Although the fully parametric Greco model did not describe precisely the entire response surface of all antifungal drug interactions, it was able to differentiate synergistic from non-synergistic interactions with a non-unit, reproducible, concentration-independent interaction parameter, including its uncertainty, without requiring replication. The Bliss independence based models resulted in mosaics of synergistic and antagonistic combinations, raising questions about the concentration-dependent nature of antifungal drug interaction. The sum of all statistically significant interactions were used as a summary interaction parameter for the entire response surface, concluding synergy or antagonism when it was positive or negative, respectively. The cutoffs of 100% and 200% were used to distinguish weak and moderate interactions, respectively in 12-16 x 8-12 checkerboard formats. Semi-parametric approaches need particular care as experimental errors are not eliminated from the entire response surface

Systems biology and bioinformatics in aging research: a workshop report

In an "aging society," health span extension is most important. As in 2010, talks in this series of meetings in Rostock-Warnemünde demonstrated that aging is an apparently very complex process, where computational work is most useful for gaining insights and to find interventions that counter aging and prevent or counteract aging-related diseases. The specific topics of this year's meeting entitled, "RoSyBA: Rostock Symposium on Systems Biology and Bioinformatics in Ageing Research," were primarily related to "Cancer and Aging" and also had a focus on work funded by the German Federal Ministry of Education and Research (BMBF). The next meeting in the series, scheduled for September 20-21, 2013, will focus on the use of ontologies for computational research into aging, stem cells, and cancer. Promoting knowledge formalization is also at the core of the set of proposed action items concluding this report. © Copyright 2012, Mary Ann Liebert, Inc