48 research outputs found
Synthetic Triterpenoids Cooperate with Tumor Necrosis Factor–Related Apoptosis-Inducing Ligand to Induce Apoptosis of Breast Cancer Cells
The Synthetic Triterpenoids, CDDO and CDDO-Imidazolide, Are Potent Inducers of Heme Oxygenase-1 and Nrf2/ARE Signaling
Use of HL-60 cell differentiation for the discovery and characterization of cancer chemopreventive agents.
Use of HL-60 cell differentiation for the discovery and characterization of cancer chemopreventive agents
Chemopreventive Activity of Vitamin E in Breast Cancer: A Focus on γ- and δ-Tocopherol
Vitamin E consists of eight different variants: α-, β-, γ-, and δ-tocopherols (saturated phytyl tail) and α-, β-, γ-, and δ-tocotrienols (unsaturated phytyl tail). Cancer prevention studies with vitamin E have primarily utilized the variant α-tocopherol. To no avail, a majority of these studies focused on variant α-tocopherol with inconsistent results. However, γ-tocopherol, and more recently δ-tocopherol, have shown greater ability to reduce inflammation, cell proliferation, and tumor burden. Recent results have shown that γ-enriched mixed tocopherols inhibit the development of mammary hyperplasia and tumorigenesis in animal models. In this review, we discuss the possible differences between the variant forms, molecular targets, and cancer-preventive effects of tocopherols. We recommend that a γ-enriched mixture, γ- and δ-tocopherol, but not α-tocopherol, are promising agents for breast cancer prevention and warrant further investigation
Histone Demethylase KDM7A Contributes to the Development of Hepatic Steatosis by Targeting Diacylglycerol Acyltransferase 2
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. While the development of NAFLD is correlated with aberrant histone methylation, modifiers of histone methylation involved in this event remain poorly understood. Here, we studied the functional role of the histone demethylase KDM7A in the development of hepatic steatosis. KDM7A overexpression in AML12 cells upregulated diacylglycerol acyltransferase 2 (DGAT2) expression and resulted in increased intracellular triglyceride (TG) accumulation. Conversely, KDM7A knockdown reduced DGAT2 expression and TG accumulation, and significantly reversed free fatty acids-induced TG accumulation. Additionally, adenovirus-mediated overexpression of KDM7A in mice resulted in hepatic steatosis, which was accompanied by increased expression of hepatic DGAT2. Furthermore, KDM7A overexpression decreased the enrichment of di-methylation of histone H3 lysine 9 (H3K9me2) and H3 lysine 27 (H3K27me2) on the promoter of DGAT2. Taken together, these results indicate that KDM7A overexpression induces hepatic steatosis through upregulation of DGAT2 by erasing H3K9me2 and H3K27me2 on the promoter
Peroxisome Proliferator-Activated Receptor-γ-Independent Repression of Collagenase Gene Expression by 2-Cyano-3,12-dioxooleana-1,9-dien-28-oic Acid and Prostaglandin 15-Deoxy-Δ(12,14) J 2
Design and Synthesis of Tricyclic Compounds with Enone Functionalities in Rings A and C:Â A Novel Class of Highly Active Inhibitors of Nitric Oxide Production in Mouse Macrophages
Diastereotopic and Deuterium Effects in Gemini
Changing the geminal methyl groups
on 1α,25-dihydroxyvitamin
D<sub>3</sub> and its analogues to the deuterio versions generally
improves the bioactivity. Derivatives of 1α,25-dihydroxyvitamin
D<sub>3</sub> with two chains emanating at C20, commonly referred
to as gemini, are subject to the same phenomenon. Additionally, gemini
with different side chains are susceptible to bioactivity differentials
where the C17–C20 threo configuration usually imparts higher
activity than the corresponding erythro arrangement. In an effort
to analyze the deuterium effect on gemini with minimal diastereotopic
distortion, we synthesized gemini with equal side chains but introduced
deuterium diastereospecifically on either chain. We solved the crystal
structures of these compounds in the zebra fish zVDR ligand binding
domain as complexes with NCoA-2 coactivator peptide and correlated
the findings with growth inhibition in a breast cancer cell line