A Comprehensive Study for building Resource Information Infrastructure oriented to Digital Archives

科学研究費助成事業(科学研究費補助金)研究成果報告書：基盤研究(A)2010-2012研究課題番号：2224002

Documenting Spatial and Tempral Information for Heritage Preservation: A Case Study of Sri Lanka

Cultural Heritage Properties (CHPs) around the world have been altered or destroyed due to various unforeseen factors, both natural and human-made. Consequently, as a preparedness approach around such disasters, documenting the CHPs are crucial to any efforts to repair, rebuild or relocate them. With advancements in digital technologies, integrating them into our documentation to improve heritage preservation has become a common approach. Here the main concern is on Spatial and Temporal (ST) information and the paper proposes that with recent developments in the field of Geospatial technologies, heritage preservation can be enhanced and improved by documenting ST information parallel to the other information resources. The study area of this research is the CHPs in Sri Lanka. The paper investigates the present condition of the ST information in the heritage arena and the challenges associated with the same. Finally, the paper suggests a metadata standard to acquire primary level ST information as the initial ST documentation strategy. This can be extended further as a complete standard of good practice for CHP documentation in Sri Lanka.The 12th annual meeting of the Document Academy (DOCAM), 2015Days: 20-22 July, 2015Place: Sydney, AustraliaTheme: Documents Unbounde

Documenting Spatial and Temporal Information for Heritage Preservation: A Case Study of Sri Lanka

Cultural Heritage Properties (CHPs) around the world have been altered or destroyed due to various unforeseen factors, both natural and human-made. Consequently, as a preparedness approach around such disasters, documenting the CHPs are crucial to any efforts to repair, rebuild or relocate them. With advancements in digital technologies, integrating them into our documentation to improve heritage preservation has become a common approach. Here the main concern is on Spatial and Temporal (ST) information and the paper proposes that with recent developments in the field of Geospatial technologies, heritage preservation can be enhanced and improved by documenting ST information parallel to the other information resources. The study area of this research is the CHPs in Sri Lanka. The paper investigates the present condition of the ST information in the heritage arena and the challenges associated with the same. Finally, the paper suggests a metadata standard to acquire primary level ST information as the initial ST documentation strategy. This can be extended further as a complete standard of good practice for CHP documentation in Sri Lanka

A Manga Creator Support Tool Based on a Manga Production Process Model - Improving Productivity by Metadata

Manga is a Japanese style comic. Nowadays, production of manga in the digital environment is widely accepted since authoring/drawing tools on PC have become popular. However, the digitalization of manga production is only in the later stages of the whole production process. The goal of this research is to improve productivity of manga production using information technologies in the earlier stages of the process. A fundamental problem exists in information resource management in the production process, e.g., reuse of unused scenarios for a new content, revision of existing character image. In this paper we propose a manga production support tool which helps creators (re)use existing resource in the production process, e.g., annotations attached to design memos, communication history, and so on. The tool uses metadata for various resources used and created in the production process. This paper describes the background of the study and overviews the production support tool.publishedye

Promoter-Level Transcriptome Identifies Stemness Associated With Relatively High Proliferation in Pancreatic Cancer Cells

Both pancreatic intraepithelial neoplasia (PanIN), a frequent precursor of pancreatic cancer, and intraductal papillary mucinous neoplasm (IPMN), a less common precursor, undergo several phases of molecular conversions and finally develop into highly malignant solid tumors with negative effects on the quality of life. We approached this long-standing issue by examining the following PanIN/IPMN cell lines derived from mouse models of pancreatic cancer: Ptf1a-Cre; KrasG12D; p53f/+ and Ptf1a-Cre; KrasG12D; and Brg1f/f pancreatic ductal adenocarcinomas (PDAs). The mRNA from these cells was subjected to a cap analysis of gene expression (CAGE) to map the transcription starting sites and quantify the expression of promoters across the genome. Two RNA samples extracted from three individual subcutaneous tumors generated by the transplantation of PanIN or IPMN cancer cell lines were used to generate libraries and Illumina Seq, with four RNA samples in total, to depict discrete transcriptional network between IPMN and PanIN. Moreover, in IPMN cells, the transcriptome tended to be enriched for suppressive and inhibitory biological processes. In contrast, the transcriptome of PanIN cells exhibited properties of stemness. Notably, the proliferation capacity of the latter cells in culture was only minimally constrained by well-known chemotherapy drugs such as GSK690693 and gemcitabine. The various transcriptional factor network systems detected in PanIN and IPMN cells reflect the distinct molecular profiles of these cell types. Further, we hope that these findings will enhance our mechanistic understanding of the characteristic molecular alterations underlying pancreatic cancer precursors. These data may provide a promising direction for therapeutic research

G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase

金沢大学医薬保健研究域医学系Aims: The lysophospholipid mediator sphingosine-1-phosphate (S1P) activates G protein-coupled receptors (GPCRs) to induce potent inhibition of platelet-derived growth factor (PDGF)-induced Rac activation and, thereby, chemotaxis in rat vascular smooth muscle cells (VSMCs). We explored the heterotrimeric G protein and the downstream mechanism that mediated S1P inhibition of Rac and cell migration in VSMCs. Methods and results: S1P inhibition of PDGF-induced cell migration and Rac activation in VSMCs was abolished by the selective S1P2 receptor antagonist JTE-013. The C-terminal peptides of Gα subunits (Gα-CTs) act as specific inhibitors of respective G protein-GPCR coupling. Adenovirus-mediated expression of Gα12-CT, Gα13-CT, and Gα q-CT, but not that of Gαs-CT or LacZ or pertussis toxin treatment, abrogated S1P inhibition of PDGF-induced Rac activation and migration, indicating that both G12/13 and Gq classes are necessary for the S1P inhibition. The expression of Gαq-CT as well as Gα12-CT and Gα13-CT also abolished S1P-induced Rho stimulation. C3 toxin, but not a Rho kinase inhibitor or a dominant negative form of Rho kinase, abolished S1P inhibition of PDGF-induced Rac activation and cell migration. The angiotensin II receptor AT1, which robustly couples to Gq, did not mediate either Rho activation or inhibition of PDGF-induced Rac activation or migration, suggesting that activation of Gq alone was not sufficient for Rho activation and resultant Rac inhibition. However, the AT1 receptor fused to Gα12 was able to induce not only Rho stimulation but also inhibition of PDGF-induced Rac activation and migration. Phospholipase C inhibition did not affect S1P-induced Rho activation, and protein kinase C activation by a phorbol ester did not mimic S1P action, suggesting that S1P inhibition of migration or Rac was not dependent on the phospholipase C pathway. Conclusion: These observations together suggest that S1P2 mediates inhibition of Rac and migration through the coordinated action of G 12/13 and Gq for Rho activation in VSMCs. © The Author 2008.

Clinical Characteristics and Outcomes in 314 Japanese Patients with Bacterial Endophthalmitis : A Multicenter Cohort Study from J-CREST

Bacterial endophthalmitis is an intraocular infection that causes rapid vison loss. Pathogens can infect the intraocular space directly (exogenous endophthalmitis (ExE)) or indirectly (endogenous endophthalmitis (EnE)). To identify predictive factors for the visual prognosis of Japanese patients with bacterial endophthalmitis, we retrospectively examined the bacterial endophthalmitis characteristics of 314 Japanese patients and performed statistics using these clinical data. Older patients, with significantly more severe clinical symptoms, were prevalent in the ExE group compared with the EnE group. However, the final best-corrected visual acuity (BCVA) was not significantly different between the ExE and EnE groups. Bacteria isolated from patients were not associated with age, sex, or presence of eye symptoms. Genus Streptococcus, Streptococcus pneumoniae, and Enterococcus were more prevalent in ExE patients than EnE patients and contributed to poor final BCVA. The presence of eye pain, bacterial identification, and poor BCVA at baseline were risk factors for final visual impairment

Background Factors Affecting Visual Acuity at Initial Visit in Eyes with Central Retinal Vein Occlusion : Multicenter Study in Japan

Purpose: To determine the baseline characteristics of patients with central retinal vein occlusion (CRVO) that were significantly associated with the best-corrected visual acuity (BCVA) at the initial examination. Methods: This was a retrospective multicenter study using the medical records registered in 17 ophthalmological institutions in Japan. Patients with untreated CRVO (≥20-years-of-age) who were initially examined between January 2013 and December 2017 were studied. The patients’ baseline factors that were significantly associated with the BCVA at the initial examination were determined by univariate and multivariate linear regression analyses. Results: Data from 517 eyes of 517 patients were analyzed. Univariate analyses showed that an older age (r = 0.194, p < 0.001) and the right eye (r = −0.103, p < 0.019) were significantly associated with poorer BCVA at the initial visit. Multivariate analyses also showed that an older age (β = 0.191, p < 0.001) and the right eye (β = −0.089, p = 0.041) were significantly associated with poorer BCVA at the initial visit. Conclusions: The results indicate that an older age, a known strong factor, and the right eye were significantly associated with poorer BCVA at the initial visit to the hospital. These results suggest that functional and/or anatomical differences between the right and left eyes may be involved in these results