Tetra­aqua­tetra­kis­(4,4′-bipyridine dioxide-κO)terbium(III) octa­cyanido­molybdate(V)

In the title compound, [Tb(C10H8N2O2)4(H2O)4][Mo(CN)8], both metal atoms are eight-coordinated. The TbIII atom displays a dodecahedral geometry, while the MoV ion exhibits a distorted square-anti­prismatic geometry. The Tb atoms are located on a special position of site symmetry , whereas the Mo atoms are located on a twofold rotation axis. The cations are linked by O—H⋯O hydrogen bonds

Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNA

RNA interference (RNAi) has been successfully applied in suppression of hepatic cancer genes. In hepatocelluar carcinoma cell, one methionine adenosyltransferase (MAT) isozyme, MATII was found to have two catalytic subunits which were encoded by MAT2A and MAT2β respectively. During tumorigeness of hepatocelluar carcinoma, expressions of the two genes were discovered to be increased combining with a switch of MAT (form MATI to MATII), To figure out the role played by MATII in hepatic cancer, In this study, for the first time we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules specifically targeting two genes. To test the effectiveness of this system, we applied this approach to express simultaneously two different siRNA duplexes that specifically target MAT2A and MAT2β genes of hepatocelluar carcinoma respectively in HepG2 cell. Results indicated that dual siRNA could simultaneously inhibit the expression of MAT2A and MAT2β gene by 89.5% and 97.8% respectively, In addition, dual siRNA molecules were able to significantly suppress growth of hepatocelluar carcinoma cell in vitro as well as induce apoptosis which was involved in arrest cell cycle at the G1/S checkpoint and the expressions of p21, p27 and Bax

Pyrolysis gas as a carbon source for biogas production via anaerobic digestion

Carbon is an important resource for anaerobes to enhance biogas production. In this study, the possibility of using simulated pyrolysis gas (SPG) as a carbon source for biogas production was investigated. The effects of stirring speed (SS), gas holding time (GHT), and H2 addition on biomethanation of SPG were evaluated. The diversity and structure of microbial communities were also analyzed under an illumina MiSeq platform. Results indicated that at a GHT of 14 h and an SS at 400 rpm, SPG with up to 64.7% CH4could be bio-upgraded to biogas. Gas–liquid mass transfer is the limitation for SPG biomethanation. For the first time, it has been noticed that the addition of H2 can bioupgrade SPG to high quality biogas (with 91.1% CH4). Methanobacterium was considered as a key factor in all reactors. This study provides an idea and alternative way to convert lignocellulosic biomass and solid organic waste into energy (e.g., pyrolysis was used as a pretreatment to produce pyrolysis gas from biomass, and then, pyrolysis gas was bioupgraded to higher quality biogas via anaerobic digestion)

Computational Prediction of Protein-Protein Interactions of Human Tyrosinase

The various studies on tyrosinase have recently gained the attention of researchers due to their potential application values and the biological functions. In this study, we predicted the 3D structure of human tyrosinase and simulated the protein-protein interactions between tyrosinase and three binding partners, four and half LIM domains 2 (FHL2), cytochrome b-245 alpha polypeptide (CYBA), and RNA-binding motif protein 9 (RBM9). Our interaction simulations showed significant binding energy scores of −595.3 kcal/mol for FHL2, −859.1 kcal/mol for CYBA, and −821.3 kcal/mol for RBM9. We also investigated the residues of each protein facing toward the predicted site of interaction with tyrosinase. Our computational predictions will be useful for elucidating the protein-protein interactions of tyrosinase and studying its binding mechanisms

Developing the Symptoms and Functional Impairment Rating Scale:A Multi-Dimensional ADHD Scale

Objective: This research developed a practical, multi-dimensional attention deficit hyperactivity disorder (ADHD) rating scale (i.e., the Symptoms and Functional Impairment Rating Scale, SFIRS) for Chinese children, aged 6-12 years, with ADHD. Methods: The structural validity, criterion validity, internal consistency, and test-retest reliability of the scale were evaluated. Item screening was conducted with 412 ADHD patients and 322 developmentally typical controls. Results: The scale includes 44 items, divided among Hyperactivity-Impulsivity, Self-Control, Inattention, Self-Management, Academic Performance, and Social Interaction. The six-factor model showed good data fit, with each factor significantly correlated with its corresponding criterion (r=0.690-0.841). The Cronbach's α of the full scale was 0.976. Total score test-retest reliability was r=0.816 (p<0.01). Conclusion: The SFIRS thus demonstrated good reliability and validity and may be used to assess ADHD among children aged 6-12 years in China

Effects of age, sex and pathological type on the risk of multiple polyps: A Chinese teaching hospital study

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162741/2/cdd12863.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162741/1/cdd12863_am.pd

Deep Brain Magnetic Stimulation Promotes Neurogenesis and Restores Cholinergic Activity in a Transgenic Mouse Model of Alzheimer’s Disease

Alzheimer’s disease (AD) is characterized by progressive decline of memory and cognitive functions. Deep magnetic stimulation (DMS), a noninvasive and nonpharmacological brain stimulation, has been reported to alleviate stress-related cognitive impairment in neuropsychiatric disorders. Our previous study also discovered the preventive effect of DMS on cognitive decline in an AD mouse model. However, the underlying mechanism must be explored further. In this study, we investigated the effect of DMS on spatial learning and memory functions, neurogenesis in the dentate gyrus (DG), as well as expression and activity of the cholinergic system in a transgenic mouse model of AD (5XFAD). Administration of DMS effectively improved performance in spatial learning and memory of 5XFAD mice. Furthermore, neurogenesis in the hippocampal DG of DMS-treated 5XFAD mice was clearly enhanced. In addition, DMS significantly raised the level of acetylcholine and prevented the increase in acetylcholinesterase activity as well as the decrease in acetyltransferase activity in the hippocampus of 5XFAD mice. These findings indicate that DMS may be a promising noninvasive tool for treatment and prevention of AD cognitive impairment by promoting neurogenesis and enhancing cholinergic system function

Research Progress on the molecular mechanism of the Utilization of Human Milk Oligosaccharides in Bifidobacterium longum subsp. infantis and Its probiotic effect

Human milk is the most important source of nutrition in early infancy, which can meet all the nutritional needs in the first 6 months after birth. It contains many bioactive substances that can regulate the intestinal flora, promote the development of the immune system, and enhance the intestinal barrier. Human milk oligosaccharides (HMOs) are one of the active substances in human milk. They cannot be directly digested and absorbed by infants, but can be used as a prebiotic to stimulate the establishment and evolution of the gut microbiota. Bifidobacterium longum subsp. infantis is a dominant microorganism in the gut of breastfed infants, which has almost all gene clusters required for metabolizing the major HMOs, and its interaction with HMOs plays a key role in the early intestinal health of infants. This review summarizes the composition and structure of HMOs, describes the utilization of HMOs by B. longum subsp. infantis and summarizes the beneficial effects B. longum subsp. infantis exerts in infants by metabolizing HMOs, which will lay the foundation for exploring the interaction mechanism between HMOs and the gut microbiota, as well as its role in infant intestinal development and maturation