Biofouling of the hydroid Ectopleura larynx on aquaculture nets in Ireland: implications for finfish health

Peer-reviewedThe potential direct health problems posed to marine-farmed salmonids by the biofouling hydroid Ectopleura larynx (Phylum Cnidaria, Class Hydrozoa) and in situ net washing processes to remove the fouling organisms have not yet been addressed. In an attempt to address the possible impacts, the rate of E. larynx growth on aquaculture nets over a net-cleaning cycle was assessed and Atlantic salmon (Salmo salar) smolts were exposed to hydroid-biofouled nets under experimental challenge. After only 1 week of immersion, there was a high settlement of E. larynx on net panels, with the maximum growth observed after 3 week of immersion. For the challenges trials, experimental treatment groups of S. salar were exposed to hydroid net panels or loose hydroid material for 11 hours under controlled conditions. Gills were examined for signs of gross damage and assigned a histopathological gill score. Prior to the experiment, the gills were healthy and did not show signs of damage from any insult. After exposure to E. larynx, focal areas of epithelial sloughing, necrosis and haemorrhage were visible on the gills under histopathology and a maximum gill score of 4 was observed. These results are the first in an investigation of this kind and suggest that E. larynx can damage the gills of S. salar. Further work on this area is vital to develop a better understanding of the pathogenesis of the damage caused by hydroids and their long-term effects on fish health, growth and survival

Cervicovaginal immune activation in Zambian women with female genital schistosomiasis

HIV-1 infection disproportionately affects women in sub-Saharan Africa, where areas of high HIV-1 prevalence and Schistosoma haematobium endemicity largely overlap. Female genital schistosomiasis (FGS), an inflammatory disease caused by S. haematobium egg deposition in the genital tract, has been associated with prevalent HIV-1 infection. Elevated levels of the chemokines MIP-1 alpha (CCL-3), MIP-1 beta (CCL-4), IP-10 (CXCL-10), and IL-8 (CXCL-8) in cervicovaginal lavage (CVL) have been associated with HIV-1 acquisition. We hypothesize that levels of cervicovaginal cytokines may be raised in FGS and could provide a causal mechanism for the association between FGS and HIV-1. In the cross-sectional BILHIV study, specimens were collected from 603 female participants who were aged 18-31 years, sexually active, not pregnant and participated in the HPTN 071 (PopART) HIV-1 prevention trial in Zambia. Participants self-collected urine, and vaginal and cervical swabs, while CVLs were clinically obtained. Microscopy and Schistosoma circulating anodic antigen (CAA) were performed on urine. Genital samples were examined for parasite-specific DNA by PCR. Women with FGS (n=28), defined as a positive Schistosoma PCR from any genital sample were frequency age-matched with 159 FGS negative (defined as negative Schistosoma PCR, urine CAA, urine microscopy, and colposcopy imaging) women. Participants with probable FGS (n=25) (defined as the presence of either urine CAA or microscopy in combination with one of four clinical findings suggestive of FGS on colposcope-obtained photographs) were also included, for a total sample size of 212. The concentrations of 17 soluble cytokines and chemokines were quantified by a multiplex bead-based immunoassay. There was no difference in the concentrations of cytokines or chemokines between participants with and without FGS. An exploratory analysis of those women with a higher FGS burden, defined by >= 2 genital specimens with detectable Schistosoma DNA (n=15) showed, after adjusting for potential confounders, a higher Th2 (IL-4, IL-5, and IL-13) and pro-inflammatory (IL-15) expression pattern in comparison to FGS negative women, with differences unlikely to be due to chance (p=0.037 for IL-4 and p<0.001 for IL-5 after adjusting for multiple testing). FGS may alter the female genital tract immune environment, but larger studies in areas of varying endemicity are needed to evaluate the association with HIV-1 vulnerability.Cancer Signaling networks and Molecular Therapeutic

The IDEAL framework for surgical robotics: development, comparative evaluation and long-term monitoring

The next generation of surgical robotics is poised to disrupt healthcare systems worldwide, requiring new frameworks for evaluation. However, evaluation during a surgical robot’s development is challenging due to their complex evolving nature, potential for wider system disruption and integration with complementary technologies like artificial intelligence. Comparative clinical studies require attention to intervention context, learning curves and standardized outcomes. Long-term monitoring needs to transition toward collaborative, transparent and inclusive consortiums for real-world data collection. Here, the Idea, Development, Exploration, Assessment and Long-term monitoring (IDEAL) Robotics Colloquium proposes recommendations for evaluation during development, comparative study and clinical monitoring of surgical robots—providing practical recommendations for developers, clinicians, patients and healthcare systems. Multiple perspectives are considered, including economics, surgical training, human factors, ethics, patient perspectives and sustainability. Further work is needed on standardized metrics, health economic assessment models and global applicability of recommendations