15 research outputs found
The impact of gut bacteria producing long chain homologs of vitamin K2 on colorectal carcinogenesis
Effects of oral probiotic supplementation on gut Lactobacillus and Bifidobacterium populations and the clinical status of low-birth-weight preterm neonates : a multicenter randomized, double-blind, placebo-controlled trial
A new electrochemical sensor with the Refreshable Silver Liquid Amalgam Film multi-Electrode for sensitive voltammetric determination of vitamin K2 (menaquinone)
Electrochemical determination of budesonide : a common corticosteroid used to treat respiratory diseases such as COVID-19 and asthma
A high-throughput method for the quantification of iron saturation in lactoferrin preparations
Lactoferrin is considered as a part of the innate
immune system that plays a crucial role in preventing bac-
terial growth, mostly via an iron sequestration mechanism.
Recent data show that bovine lactoferrin prevents late-onset
sepsis in preterm very low birth weight neonates by serving
as an iron chelator for some bacterial strains; thus, it is very
important to control the iron saturation level during diet
supplementation. An accurate estimation of lactoferrin iron
saturation is essential not only because of its clinical appli-
cations but also for a wide range of biochemical experi-
ments. A comprehensive method for the quantification of
iron saturation in lactoferrin preparations was developed to
obtain a calibration curve enabling the determination of iron
saturation levels relying exclusively on the defined ratio of
absorbances at 280 and 466 nm (
A
280/466
). To achieve this
goal, selected techniques such as spectrophotometry, ELISA,and ICP-MS were combined. The ability to obtain samples of
lactoferrin with determination of its iron content in a simple
and fast way has beenproven tobe veryuseful. Furthermore, a
similar approach could easily be implemented to facilitate the
determination of iron saturation level for other metalloproteins
in which metal binding results in the appearance of a distinct
band in the visible part of the spectrum
Fast and reliable voltammetric determination of menaquinone (vitamin K2) produced in vitro by Bacillus subtilis cultures
The in vitro Activity of Vaginal Lactobacillus With Probiotic Properties Against Candida
Lactobacilli, the predominant vaginal microorganisms in healthy premenopausal women, control other members of the
vaginal microflora and thus protect against bacterial vaginosis and urinary tract infections. It has been claimed that some
lactobacilli are also protective against Candida vaginitis. Little is known, however, about the mechanisms by which these
lactobacilli can control vaginal populations of Candida and prevent vaginitis. To address this question, vaginal Lactobacillus
strains with known antagonistic properties against bacteria were tested for their cell surface properties, adhesion to vaginal
cell lines in vitro and antagonistic activities against Candida. A small proportion of the lactobacilli tested adhered strongly to
cultured vaginal epithelial cells and inhibited growth of Candida albicans but not of C. pseudotropicalis. This anticandidal
activity was in some Lactobacillus strains related to hydrogen peroxide (H2O2) production, but catalase treatment did not
suppress this activity in other Lactobacillus strains, suggesting alternative mechanism(s). Moreover, tested vaginal Candida
strains were resistant to relatively high concentrations of H2O2 that markedly exceeded those produced by even the most
active Lactobacillus strains