27 research outputs found

    Complete Genome Sequences of Mycobacterium smegmatis Phages Chewbacca, Reptar3000, and Riparian, Isolated in Las Vegas, Nevada

    Get PDF
    Here, we present the complete genome sequences of Mycobacterium smegmatis phages Chewbacca, Reptar3000, and Riparian, isolated from soil in Las Vegas, NV. The phages were isolated and annotated by undergraduate students enrolled in the Phage Discovery course offered by the School of Life Sciences at the University of Nevada, Las Vega

    Nitric Oxide Facilitates Delivery and Mediates Improved Outcome of Autologous Bone Marrow Mononuclear Cells in a Rodent Stroke Model

    Get PDF
    Bone marrow mononuclear cells (MNC) represent an investigational treatment for stroke. The objective of this study was to determine the relevance of vasoactive mediators, generated in response to MNC injection, as factors regulating cerebral perfusion (CP), the biodistribution of MNC, and outcome in stroke.Long Evans rats underwent transient middle cerebral artery occlusion. MNC were extracted from the bone marrow at 22 hrs and injected via the internal carotid artery or the femoral vein 2 hours later. CP was measured with MRI or continuous laser Doppler flowmetry. Serum samples were collected to measure vasoactive mediators. Animals were treated with the Nitric Oxide (NO) inhibitor, L-NAME, to establish the relevance of NO-signaling to the effect of MNC. Lesion size, MNC biodistribution, and neurological deficits were assessed.CP transiently increased in the peri-infarct region within 30 min after injecting MNC compared to saline or fibroblast control. This CP increase corresponded temporarily to serum NO elevation and was abolished by L-NAME. Pre-treatment with L-NAME reduced brain penetration of MNC and prevented MNC from reducing infarct lesion size and neurological deficits.NO generation in response to MNC may represent a mechanism underlying how MNC enter the brain, reduce lesion size, and improve outcome in ischemic stroke

    Organizing Pedagogies: Transgressing Campus-Movement Boundaries Through Radical Study and Action

    No full text
    This article considers the speculative and pedagogical character of campus abolitionist organizing. Extending education research into the knowledge (re)producing functions of radical activism, we draw upon the Black Radical Tradition to theorize the intersections of learning and imagination in both activism and education

    L-NAME inhibits the beneficial effect of MNCs.

    No full text
    <p>(A) Chronic infarct cavity at 28 days after stroke in animals treated with saline or MNCs. Animals at 24 hrs after stroke received saline followed by IV MNCs (10 million cells), or L-NAME followed by IV MNCs (10 million cells), or saline only. Lesion size was reduced by MNCs at 28 days after stroke. However, pre-treatment with L-NAME before MNC infusion abolished this reduction in lesion size (§p<0.05 compared with saline). The lesion size was calculated as a percent of the contralateral cortex N = 8 per group. (B) Neurological deficits serially evaluated after stroke in animals treated with saline IP followed by IV infusion of MNCs or L-NAME IP followed by IV infusion of MNCs. At 28 days, animals treated with saline followed by MNCs showed a significant reduction in deficits compared with animals treated with L-NAME+MNCs or compared with animals treated with saline only (§p<0.05). The x-axis represents the time in days after stroke. Baseline refers to the time point before stroke. N = 8 per group.</p
    corecore