Preclinical Toxicology Studies of Recombinant Human Platelet-Derived Growth Factor-BB Either Alone or in Combination with Beta-Tricalcium Phosphate and Type I Collagen

Human platelet-derived growth factor-BB (hPDGF-BB) is a basic polypeptide growth factor released from platelets at the injury site. It is a multifunctional molecule that regulates DNA synthesis and cell division and induces biological effects that are implicated in tissue repair, atherosclerosis, inflammatory responses, and neoplastic diseases. This paper is an overview of the toxicology data generated from a broad testing platform to determine bone, soft tissue, and systemic responses following administration of rhPDGF-BB. Moreover, the systemic and local toxicity of recombinant human PDGF-BB (rhPDGF-BB) in combination with either beta-tricalcium phosphate (β-TCP) or collagen combined with β-TCP was studied to determine dermal sensitization, irritation, intramuscular tissue responses, pyrogenicity, genotoxicity, and hemolytic properties. All data strongly suggest that rhPDGF-BB either alone or in combination with β-TCP or collagen with β-TCP is biocompatible and has neither systemic nor local toxicity, supporting its safe use in enhancing wound healing in patients

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Self-Folding Textiles through Manipulation of Knit Stitch Architecture

This research presents a preliminary study on finding predictable methods of controlling the self-folding behaviors of weft knit textiles for use in the development of smart textiles and garment devices, such as those with shape memory, auxetic behavior or transformation abilities. In this work, Shima Seiki SDS-One Apex computer-aided knitting technology, Shima Seiki industrial knitting machines, and the study of paper origami tessellation patterns were used as tools to understand and predict the self-folding abilities of weft knit textiles. A wide range of self-folding weft knit structures was produced, and relationships between the angles and ratios of the knit and purl stitch types were determined. Mechanical testing was used as a means to characterize differences produced by stitch patterns, and to further understand the relationships between angles and folding abilities. By defining a formulaic method for predicting the nature of the folds that occur due to stitch architecture patterns, we can better design self-folding fabrics for smart textile applications

Understanding the importance of endosporulation methods for generating endospores that can resist harsh conditions and produce calcite in bio self-healing of concrete

Vegetative cells used for the concrete bio self-healing process often face threatening environmental conditions such as extreme temperature, pH, salinity, shear stress, and starvation during the hardening process and the service life of the concrete. These conditions can eventually lead to cell death. Since endospores are likely to remain dormant for prolonged periods and can survive, germinate, and grow under inhospitable conditions, they are a suitable bacterial phenotype to introduce into concrete for microbial-inducing calcite precipitation. This study investigated how different endosporulation methods affect the endosporulation ratio (i.e., the fraction of vegetative cells that are converted to endospores during endosporulation), as well as the germination ratio (i.e., the fraction of endospores that are converted to vegetative cells following germination) and the microbial-induced calcite precipitation (MICP) performance of germinated endospores after facing harsh conditions of concrete, specifically, freeze and that cycling. Results from this study show that thermal shock followed by cell incubation in alkaline conditions leads to increased sporulation and germination ratios. It was also observed that freeze and thaw cycling had negligible effects on calcite production by endospores, while exposure of vegetative cells to these harsh conditions led to not only less biomass and calcite production but also to a lower mass of calcite produced per mass of cells, as determined by thermogravimetric analysis (TGA). The results from this study provide key insights into improving methods for endosporulation and germination to effectively use them for bio self-healing applications in concrete