Phosphorylation of Vasodilator-Stimulated Phosphoprotein (VASP) dampens hepatic ischemia-reperfusion injury

Recent work has demonstrated that the formation of platelet neutrophil complexes (PNCs) affects inflammatory tissue injury. Vasodilator-stimulated phosphoprotein (VASP) is crucially involved into the control of PNC formation and myocardial reperfusion injury. Given the clinical importance of hepatic IR injury we pursued the role of VASP during hepatic ischemia followed by reperfusion. We report here that VASP−/− animals demonstrate reduced hepatic IR injury compared to wildtype (WT) controls. This correlated with serum levels of lactate dehydrogenase (LDH), aspartate (AST) and alanine (ALT) aminotransferase and the presence of PNCs within ischemic hepatic tissue and could be confirmed using repression of VASP through siRNA. In studies employing bone marrow chimeric mice we identified hematopoietic VASP to be of crucial importance for the extent of hepatic injury. Phosphorylation of VASP on Ser153 through Prostaglandin E1 or on Ser235 through atrial natriuretic peptide resulted in a significant reduction of hepatic IR injury. This was associated with a reduced presence of PNCs in ischemic hepatic tissue. Taken together, these studies identified VASP and VASP phosphorylation as crucial target for future hepatoprotective strategies

The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro

Background Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes in vitro. Methods Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions in vitro were investigated by transfection of a specific siRNA. Results Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli in vitro. Conclusions The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. In vitro experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration

BlockNet Report: Exploring the Blockchain Skills Concept and Best Practice Use Cases

In order to explore the practical potential and needs of interdisciplinary knowledge and competence requirements of Blockchain technology, the project activity "Development of Interdisciplinary Blockchain Skills Concept" starts with the literature review identifying the state of the art of Blockchain in Supply Chain Management and Logistics, Business and Finance, as well as Computer Science and IT-Security. The project activity further explores the academic and industry landscape of existing initiatives in education which offer Blockchain courses. Moreover, job descriptions and adverts are analyzed in order to specify today's competence requirements from enterprises. To discuss and define the future required competence, expert workshops are organized to validate the findings by academic experts. Based on the research outcome and validation, an interdisciplinary approach for Blockchain competence is developed. A second part focuses on the development of the Blockchain Best Practices activity while conducting qualitative empirical research based on case studies with industry representatives. Therefore, company interviews, based on the theoretical basis of Output 1, explore existing Blockchain use cases in different sectors. Due to the interdisciplinary importance of Blockchain technology, these skills will be defined by different perspectives of Blockchain from across multiple mentioned disciplines. The use cases and companies for the interviews will be selected based on various sampling criteria to gain results valid for a broad scale. The analysis of the various use cases will be conducted and defined in a standardized format to identify the key drivers and competence requirements for Blockchain technology applications and their adoption. On the one hand, this approach ensures comparability, on the other hand, it facilitates the development of a structured and systematic framework.Comment: arXiv admin note: text overlap with arXiv:2102.0322

Identifying rare chaotic and regular trajectories in dynamical systems with Lyapunov weighted path sampling

Depending on initial conditions, individual finite time trajectories of dynamical systems can have very different chaotic properties. Here we present a numerical method to identify trajectories with atypical chaoticity, pathways that are either more regular or more chaotic than average. The method is based on the definition of an ensemble of trajectories weighted according to their chaoticity, the Lyapunov weighted path ensemble. This ensemble of trajectories is sampled using algorithms borrowed from transition path sampling, a method originally developed to study rare transitions between long-lived states. We demonstrate our approach by applying it to several systems with numbers of degrees of freedom ranging from one to several hundred and in all cases the algorithm found rare pathways with atypical chaoticity. For a double-well dimer embedded in a solvent, which can be viewed as simple model for an isomerizing molecule, rare reactive pathways were found for parameters strongly favoring chaotic dynamics.Comment: 8 pages, 5 figure

Genome information processing by the INO80 chromatin remodeler positions nucleosomes [preprint]

The fundamental molecular determinants by which ATP-dependent chromatin remodelers organize nucleosomes across eukaryotic genomes remain largely elusive. Here, chromatin reconstitutions on physiological, whole-genome templates reveal how remodelers read and translate genomic information into nucleosome positions. Using the yeast genome and the multi-subunit INO80 remodeler as a paradigm, we identify DNA shape/mechanics encoded signature motifs as sufficient for nucleosome positioning and distinct from known DNA sequence preferences of histones. INO80 processes such information through an allosteric interplay between its core- and Arp8-modules that probes mechanical properties of nucleosomal and linker DNA. At promoters, INO80 integrates this readout of DNA shape/mechanics with a readout of co-evolved sequence motifs via interaction with general regulatory factors bound to these motifs. Our findings establish a molecular mechanism for robust and yet adjustable +1 nucleosome positioning and, more generally, remodelers as information processing hubs that enable active organization and allosteric regulation of the first level of chromatin

ExacTrac Dynamic workflow evaluation: Combined surface optical/thermal imaging and X‐ray positioning

In modern radiotherapy (RT), especially for stereotactic radiotherapy or stereotactic radiosurgery treatments, image guidance is essential. Recently, the ExacTrac Dynamic (EXTD) system, a new combined surface-guided RT and image-guided RT (IGRT) system for patient positioning, monitoring, and tumor targeting, was introduced in clinical practice. The purpose of this study was to provide more information about the geometric accuracy of EXTD and its workflow in a clinical environment. The surface optical/thermal- and the stereoscopic X-ray imaging positioning systems of EXTD was evaluated and compared to cone-beam computed tomography (CBCT). Additionally, the congruence with the radiation isocenter was tested. A Winston Lutz test was executed several times over 1 year, and repeated end-to-end positioning tests were performed. The magnitude of the displacements between all systems, CBCT, stereoscopic X-ray, optical-surface imaging, and MV portal imaging was within the submillimeter range, suggesting that the image guidance provided by EXTD is accurate at any couch angle. Additionally, results from the evaluation of 14 patients with intracranial tumors treated with open-face masks are reported, and limited differences with a maximum of 0.02 mm between optical/thermal- and stereoscopic X-ray imaging were found. As the optical/thermal positioning system showed a comparable accuracy to other IGRT systems, and due to its constant monitoring capability, it can be an efficient tool for detecting intra-fractional motion and for real-time tracking of the surface position during RT