410 research outputs found
Nonlinear elasticity of stiff biopolymers connected by flexible linkers
Networks of the biopolymer actin, cross-linked by the compliant protein filamin, form soft gels. They can, however, withstand large shear stresses due to their pronounced nonlinear elastic behavior. The nonlinear elasticity can be controlled by varying the number of cross-links per actin filament. We propose and test a model of rigid filaments decorated by multiple flexible linkers that is in quantitative agreement with experiment. This allows us to estimate loads on individual cross-links, which we find to be less than 10 pN. Β© 2009 The American Physical Society
On the form of growing strings
Patterns and forms adopted by Nature, such as the shape of living cells, the
geometry of shells and the branched structure of plants, are often the result
of simple dynamical paradigms. Here we show that a growing self-interacting
string attached to a tracking origin, modeled to resemble nascent polypeptides
in vivo, develops helical structures which are more pronounced at the growing
end. We also show that the dynamic growth ensemble shares several features of
an equilibrium ensemble in which the growing end of the polymer is under an
effective stretching force. A statistical analysis of native states of proteins
shows that the signature of this non-equilibrium phenomenon has been fixed by
evolution at the C-terminus, the growing end of a nascent protein. These
findings suggest that a generic non-equilibrium growth process might have
provided an additional evolutionary advantage for nascent proteins by favoring
the preferential selection of helical structures.Comment: 4 pages, 3 figures. Accepted for publication in Phys. Rev. Let
Dynamic Phase Transitions in Cell Spreading
We monitored isotropic spreading of mouse embryonic fibroblasts on
fibronectin-coated substrates. Cell adhesion area versus time was measured via
total internal reflection fluorescence microscopy. Spreading proceeds in
well-defined phases. We found a power-law area growth with distinct exponents
a_i in three sequential phases, which we denote basal (a_1=0.4+-0.2), continous
(a_2=1.6+-0.9) and contractile (a_3=0.3+-0.2) spreading. High resolution
differential interference contrast microscopy was used to characterize local
membrane dynamics at the spreading front. Fourier power spectra of membrane
velocity reveal the sudden development of periodic membrane retractions at the
transition from continous to contractile spreading. We propose that the
classification of cell spreading into phases with distinct functional
characteristics and protein activity patterns serves as a paradigm for a
general program of a phase classification of cellular phenotype. Biological
variability is drastically reduced when only the corresponding phases are used
for comparison across species/different cell lines.Comment: 4 pages, 5 figure
Stress-Dependent Elasticity of Composite Actin Networks as a Model for Cell Behavior
Networks of filamentous actin cross-linked with the actin-binding protein filamin A exhibit remarkable strain stiffening leading to an increase in differential elastic modulus by several orders of magnitude over the linear value. The variation of the frequency dependence of the differential elastic and loss moduli as a function of prestress is consistent with that observed in living cells, suggesting that cell elasticity is always measured in the nonlinear regime, and that prestress is an essential control parameter
Self-organized Vortex State in Two-dimensional Dictyostelium Dynamics
We present results of experiments on the dynamics of Dictyostelium discoideum
in a novel set-up which constraints cell motion to a plane. After aggregation,
the amoebae collect into round ''pancake" structures in which the cells rotate
around the center of the pancake. This vortex state persists for many hours and
we have explicitly verified that the motion is not due to rotating waves of
cAMP. To provide an alternative mechanism for the self-organization of the
Dictyostelium cells, we have developed a new model of the dynamics of
self-propelled deformable objects. In this model, we show that cohesive energy
between the cells, together with a coupling between the self-generated
propulsive force and the cell's configuration produces a self-organized vortex
state. The angular velocity profiles of the experiment and of the model are
qualitatively similar. The mechanism for self-organization reported here can
possibly explain similar vortex states in other biological systems.Comment: submitted to PRL; revised version dated 3/8/9
Hypothesis: are neoplastic macrophages/microglia present in glioblastoma multiforme?
Most malignant brain tumours contain various numbers of cells with characteristics of activated or dysmorphic macrophages/microglia. These cells are generally considered part of the tumour stroma and are often described as TAM (tumour-associated macrophages). These types of cells are thought to either enhance or inhibit brain tumour progression. Recent evidence indicates that neoplastic cells with macrophage characteristics are found in numerous metastatic cancers of non-CNS (central nervous system) origin. Evidence is presented here suggesting that subpopulations of cells within human gliomas, specifically GBM (glioblastoma multiforme), are neoplastic macrophages/microglia. These cells are thought to arise following mitochondrial damage in fusion hybrids between neoplastic stem cells and macrophages/microglia
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