Periferinių kraujagyslių fluorescencijos spektrų tyrimas

We have examined the effects of hypodynamic stress (as one of the risk factors for cardiovascular disease) on the central ear arteries on rabbits and associated arterial injury. A 48-day hypodynamic stress was provoked in Chinchilla male rabbits by confining them to a confined space with a limited potential for movement. The intima of the central ear arteries was examined by autofluorescence and electron microscopy. Laser-induced fluorescence was examined during excitation at a range of wavelengths 320–360 mm at the absorption maxima of several fluorescing connective tissue proteins. Changes in fluorescence profiles of an intact artery and an artery affected by hypodynamic stress were detected in the spectral range from 370 to 550 nm. Spectroscopic examination revealed a decreased relative fluorescence intensity at around 410 nm and red-shifted fluorescence emission for affected artery wall in comparison with intact artery intima. Ultrastructural studies indicated that the internal elastic lamina and subendothelial layer were disintegrated or lessening, and cholesterol had accumulated in the inner wall of central ear arteries in stress-affected rabbits. Initial results show that structural changes of the central ear artery intima due to hypodynamic stress can be monitored using fluorescence spectroscopy

Periferinių kraujagyslių fluorescencijos spektrų tyrimas

We have examined the effects of hypodynamic stress (as one of the risk factors for cardiovascular disease) on the central ear arteries on rabbits and associated arterial injury. A 48-day hypodynamic stress was provoked in Chinchilla male rabbits by confining them to a confined space with a limited potential for movement. The intima of the central ear arteries was examined by autofluorescence and electron microscopy. Laser-induced fluorescence was examined during excitation at a range of wavelengths 320–360 mm at the absorption maxima of several fluorescing connective tissue proteins. Changes in fluorescence profiles of an intact artery and an artery affected by hypodynamic stress were detected in the spectral range from 370 to 550 nm. Spectroscopic examination revealed a decreased relative fluorescence intensity at around 410 nm and red-shifted fluorescence emission for affected artery wall in comparison with intact artery intima. Ultrastructural studies indicated that the internal elastic lamina and subendothelial layer were disintegrated or lessening, and cholesterol had accumulated in the inner wall of central ear arteries in stress-affected rabbits. Initial results show that structural changes of the central ear artery intima due to hypodynamic stress can be monitored using fluorescence spectroscopy

Denosumab densitometric changes assessed by quantitative computed tomography at the spine and hip in postmenopausal women with osteoporosis

FREEDOM was a phase 3 trial in 7808 women aged 60-90 yr with postmenopausal osteoporosis. Subjects received placebo or 60 mg denosumab subcutaneously every 6 mo for 3 yr in addition to daily calcium and vitamin D. Denosumab significantly decreased bone turnover; increased dual-energy X-ray absorptiometry (DXA) areal bone mineral density (aBMD); and significantly reduced new vertebral, nonvertebral, and hip fractures. In a subset of women (N = 209), lumbar spine, total hip, and femoral neck volumetric BMD (vBMD) were assessed by quantitative computed tomography at baseline and months 12, 24, and 36. Significant improvement from placebo and baseline was observed in aBMD and vBMD in the denosumab-treated subjects at all sites and time points measured. The vBMD difference from placebo reached 21.8%, 7.8%, and 5.9%, respectively, for the lumbar spine, total hip, and femoral neck at 36 mo (all p < 0.0001). Compared with placebo and baseline, significant increases were also observed in bone mineral content (BMC) at the total hip (p < 0.0001) largely related to significant BMC improvement in the cortical compartment (p < 0.0001). These results supplement the data from DXA on the positive effect of denosumab on BMD in both the cortical and trabecular compartments