Preparation and coordination chemistry of novel derivatised sulfimides

Treatment of [1,4-(PhS)2C6H4] and [1,2-(PhS)2C6H4] with the appropriate amount of O-mesitylenesulfonylhydroxylamine (MSH) yields the corresponding protonated sulfimides, both of which may be deprotonated with 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) to give the hydrated free sulfimides [1,4-(PhS{NH2})2C6H4] and [1,2-(PhS{NH2})C6H4PhS]. It would seem that both products display the ability to give two distinct types of material, anhydrous and hydrated forms. The hydrated type [1,4-(PhS{NH2})2C6H4].2H2O has been found by crystallography to form an extended array system; whereas the crystal structure of the dehydrate exhibits extra structural "rigidity" brought about by having two hydrogen-bonded sulfimide units per molecule. The ligand [1,2-(PhS{NH2})C6H4PhS] also exhibits two distinct types of formation, one a dehydrate (exhibited as an oil) and the other a hydrated type (present as a crystalline solid). [Continues.

CD 552 Christian Education Seminar: Adult Ministry

During the seminar participants will explore: * Biblical mandates for an adequate response to the elderly * Demographics of aging and the affect on congregational life * Ministry by and with Senior Adults; intergenerational involvement and ministry; need for lifelong learning * Ministry to Senior Adults; Visiting in the Nursing Home; ministry to those living alone or with family; addressing needs of caregivers of older adults * Loss and Grief Issues of Older Adults * Understanding the Aging Process and its Many Challenges * Meeting the spiritual needs of those affected by dementia and cognitive loss * A review of what various denominational groups are doing to address the aging phenomenonhttps://place.asburyseminary.edu/syllabi/3105/thumbnail.jp

Relating to reading : a psychosocial exploration of the experiences of young people who find reading difficult

Although research studies are plentiful regarding the cognitive aspects of children’s and young people’s reading difficulties, surprisingly few consider the emotional or relational impact of those difficulties. Those which do rarely invite young people to share their experiences of struggling to master this fundamental life skill. This exploratory, qualitative study used a psychosocial methodology to explore the reading experiences of young people who find reading difficult. A psychosocial ontology and epistemology gave equal consideration to the meaning constructed through participants’ social interactions and the unconscious psychological processes at work for participant and researcher, and facilitated an understanding of the emotional experience of each participant. Semi-structured interviews were conducted with two 12-year-old boys (UK school year 8) with persistent reading difficulties, using Free Association Narrative Interview (FANI) techniques. Each boy was interviewed twice, creating his own narrative in response to initial questions or prompts. The boys were also observed twice in a typical English lesson, using principles of infant observation. A reflective research diary was used to record the researcher’s personal responses to all aspects of the research. Interview data were analysed using thematic analysis. Reflective process notes from each observation provided an additional perspective on each boy’s experiences. Psychosocial research supervision enabled a reflexive stance to be maintained, holding in mind the ‘whole’ person, acknowledging the researcher as psychologically defended, and considering the relevance and appropriateness of themes as they emerged. Semantic and latent themes specific to each participant were identified. Although the aim was not to generalise between the boys’ experiences, similarities were found within the following areas: ‘(un)helpful helping’, ‘believed and understood?’, ‘lazy, dumb and stupid’, and ‘smarter sisters’. The findings provide a rich account of participants’ experiences as ‘struggling readers’. Strengths and limitations of the study are discussed, together with implications for teaching and Educational Psychology practice

Knowing the team around your patient

There are many different professionals who all contribute to the health and support of patients. Understanding each one’s different specialty is important, so that support workers know who the patient needs to be involved in their care—and when. The Code of Conduct for Healthcare Support Workers and Adult Social Care Workers in England (Skills for Care and Skills for Health, 2013) identifies that working in collaboration with colleagues is an important part of the support worker’s role. Within section three and four it highlights three key areas: support workers must ‘recognise and respect the roles and expertise of your colleagues both in the team and from other agencies and disciplines, and work in partnership with them’; ‘work openly and cooperatively with colleagues, including those from other disciplines and agencies, and treat them with respect’; and ‘communicate effectively and consult with your colleagues as appropriate’. These three keys areas of partnership working, respect and communication will be explored and discussed in this article

Evidence for Psychiatric and Mental Health Nursing Interventions: An Update (2011 through 2015)

This state-of-the-evidence review summarizes characteristics of intervention studies published from January 2011 through December 2015, in five psychiatric nursing journals. Of the 115 intervention studies, 23 tested interventions for mental health staff, while 92 focused on interventions to promote the well-being of clients. Analysis of published intervention studies revealed 92 intervention studies from 2011 through 2015, compared with 71 from 2006 through 2010, and 77 from 2000 through 2005. This systematic review identified a somewhat lower number of studies from outside the United States; a slightly greater focus on studies of mental health professionals compared with clients; and a continued trend for testing interventions capturing more than one dimension. Though substantial progress has been made through these years, room to grow remains. In this article, the authors discuss the background and significance of tracking the progress of intervention research disseminated within the specialty journals, present the study methods used, share their findings, describe the intervention domains and nature of the studies, discuss their findings, consider the implications of these studies, and conclude that continued track of psychiatric and mental health nursing intervention research is essential

Association of slow darkening gene 'SD' with grain quality traits in carioca bean and new candidate marker.

The seed darkens during storage becoming less acceptable to consumers and depreciation of the economic value. Cultivars with delayed grain darkening associated with reduced cooking time after storage will be advantageous for the farmer and seed dealers. Grain storage for longer periods allows flexibility, i.e., the producer can await better prices on the market

Photodynamic inactivation of non-enveloped RNA viruses

We recently reported the photodynamic inactivation (PDI) of bacteriophage MS2 with a photosensitiser- 5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin- tetra- p-toluene sulfonate (TMPyP) in solution and concluded that the A-protein of the virus is the main target of inactivation. Here, we have extended these studies and carried out PDI of bacteriophage Qβ, bovine enterovirus 2 (BEV-2) and type 1 murine norovirus (MNV-1). The rate of inactivation observed was in the order MS2 > Qβ > MNV-1 > BEV-2. Data suggested that TMPyP-treatment could also target the viral genome as well as result in disintegration/disassembly of viral particles. Although emergence of viral drug resistance is a well-documented phenomenon, it was not possible to generate PDI-resistant MS2. However, emergence of a mutation in the lysis protein was detected after serial exposure to PDI

An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells.

Enteroviruses comprise a large group of mammalian pathogens that includes poliovirus. Pathology in humans ranges from sub-clinical to acute flaccid paralysis, myocarditis and meningitis. Until now, all of the enteroviral proteins were thought to derive from the proteolytic processing of a polyprotein encoded in a single open reading frame. Here we report that many enterovirus genomes also harbour an upstream open reading frame (uORF) that is subject to strong purifying selection. Using echovirus 7 and poliovirus 1, we confirmed the expression of uORF protein in infected cells. Through ribosome profiling (a technique for the global footprinting of translating ribosomes), we also demonstrated translation of the uORF in representative members of the predominant human enterovirus species, namely Enterovirus A, B and C. In differentiated human intestinal organoids, uORF protein-knockout echoviruses are attenuated compared to the wild-type at late stages of infection where membrane-associated uORF protein facilitates virus release. Thus, we have identified a previously unknown enterovirus protein that facilitates virus growth in gut epithelial cells-the site of initial viral invasion into susceptible hosts. These findings overturn the 50-year-old dogma that enteroviruses use a single-polyprotein gene expression strategy and have important implications for the understanding of enterovirus pathogenesis.This work was supported by Wellcome Trust grant [106207] and European Research Council grant [646891] to A.E.F; and Wellcome Trust grants 097997/Z/11/Z and 207498/Z/17/Z to I.G

Development of an ELISA to distinguish between foot-and-mouth disease virus infected and vaccinated animals utilising the viral non-structural protein 3ABC

Introduction. Foot-and-mouth disease (FMD) is a highly contagious and economically devastating viral disease of livestock and is endemic in much of Asia, including Pakistan. Vaccination is used to control disease outbreaks and sensitive diagnostic methods which can differentiate infected animals from vaccinated animals (DIVA) are essential for monitoring the effectiveness of disease control programmes. Tests based on the detection of the non-structural protein (NSP) 3ABC are reliable indicators of virus replication in infected and vaccinated populations. Hypothesis/Gap statement. Diagnosis of FMD is expensive using commercial ELISA kits, yet is essential for controlling this economically-important disease. Aim. The development of a low-cost diagnostic ELISA, using protein made in Escherichia coli . Methodology. In this study, the viral precursor protein 3ABC (r3ABC) was expressed in E. coli , solubilised using detergent and purified using nickel affinity chromatography. The fusion protein contained an attenuating mutation in the protease and a SUMO tag. It was characterised by immunoblotting and immunoprecipitation, which revealed antigenicity against virus-specific polyclonal sera. Using r3ABC, an indirect ELISA was developed and evaluated using field sera from healthy/naïve, vaccinated and infected animals. Results. The diagnostic sensitivity and specificity of the r3ABC in-house ELISA were 95.3 and 96.3% respectively. The ELISA was validated through comparison with the commercially available ID Screen FMD NSP competition kit. Results indicated good concordance rates on tested samples and high agreement between the two tests. Conclusion. The ELISA described here can effectively differentiate between infected and vaccinated animals and represents an important low cost tool for sero-surveillance and control of FMD in endemic settings