24 research outputs found
Efekti IL-17 na funkcionalnu aktivnost Äelija periferne krvi
Interleukin-17 (IL-17) is a proinflammatory cytokine produced mainly by activated CD4+ and CD8+ T cells, while its specific receptor is ubiquitously distributed. The inflammatory capacity of IL-17 is based on its ability to stimulate a wide range of stromal cells to produce and release a number of proinflammatory mediators, some with a known impact on hematopoiesis particularly granulopoiesis. Recent data indicate a role for IL-17 in the pathogenesis of several inflammatory diseases, transplant rejection and tumor growth. The purpose of this study was to determine functional responses including the respiratory burst, nitric oxide (NO) production, adhesiveness and metabolical activity/viability of human peripheral blood leukocytes (total white blood cells, mononuclear cells and granulocytes) from healthy donors in the presence of recombinant human (rh)IL-17. The obtained results showed that rhIL-17 did not induce significant changes in the respiratory burst, NO production, and metabolical activity of each peripheral blood cell fraction the tested, while a slight increase in phorbol-12-myristate-13-acetate (PMA) stimulated adhesiveness of granulocytes and mononuclear cells was noted. The absence of significant changes in tested functional activities of various peripheral blood cells suggests that IL-17 does not express its proinflammatory ability in steady-state, since the requirement for its action really does not exist.Interleukin 17 (IL-17) je proinflamatorni citokin koga produkuju aktivirane CD4+ i CD8+ T Äelije, dok je njegov receptor ubikvitarno distribuiran. Inflamatorni kapacitet IL-17 se zasniva na njegovoj sposobnosti da stimuliÅ”e Å”irok spektar stromalnih Äelija da produkuju i oslobaÄaju razliÄite proinflamatorne medijatore, meÄu kojima neki imaju efekte na hematopoezu posebno granulopoezu. DosadaÅ”nji podaci ukazuju na ulogu IL-17 u patogenezi razliÄitih inflamatornih bolesti, odbacivanju transplanta i razvoju tumora. Cilj ovog rada je bio da se odrede funkcionalni odgovori, ukljuÄujuÄi respiratorni prasak, produkciju azot monoksida (NO), adhezivnost i metaboliÄku aktivnost/vijabilnost razliÄitih Äelija periferne krvi (ukupnih leukocita, mononuklearnih Äelija i granulocita) zdravih donora, u prisustvu IL-17. Dobijeni rezultati su ukazali da IL-17 ne dovodi do znaÄajnih promena respiratornog praska, produkcije NO i metaboliÄke aktivnosti Äelija periferne krvi, ali da uzrokuje blago poveÄanje forbol-12-miristat-13-acetat (PMA) stimulisane adhezivnosti granulocita i mononuklearnih Äelija. Odsustvo znaÄajnih promena u ispitivanim funkcionalnim aktivnostima razliÄitih Äelija periferne krvi, ukazuje da IL-17 ne eksprimira proinflamatorno dejstvo kod zdravih osoba, jer najverovatnije i ne postoji potreba za njegovim delovanjem
Effect of extended oral administration of eugenol on hematological and some biochemical blood parameters in rats
Ispitivano je moguÄe hematotoksiÄno, hepatotoksiÄno i nefrotoksiÄano dejstvo eugenola
kod pacova posle dvonedeljne i Äetvoronedeljne kontinuirane p.o. primene.
Ogled je izveden na 72 mužjaka pacova soja Wistar podeljenih u Å”est grupa. Äetiri
grupe tretirane su razliÄitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg tm/dan,
200 mg/kg tm/dan i 400 mg/kg tm/dan). Peta, kontrolna grupa dobijala je vehikulum
(0,5% metil-celuloza, 20% propilen-glikol i voda), a Ŕesta je bila apsolutna, netretirana
kontrola. Eugenol i vehikulum aplikovani su gastriÄnom sondom, svakodnevno tokom
Äetiri nedelje u koliÄini od 1 ml/100 g telesne mase pacova. Krv je uzorkovana
kardijalnom punkcijom anestetisanih pacova 14. i 28. dana ogleda u cilju odreÄivanja
hematoloŔkih i biohemijskih parametara krvi: hematokrit, broj eritrocita, leukocita i
trombocita, leukocitna formula, koncentracija hemoglobina, MCV, MCH, koncentracija
proteina, albumina, uree, kreatinina, kao i aktivnost alanin-aminotransferaze (ALT),
alkalne fosfataze (ALP) i kreatin-kinaze (CK). Rezultati su pokazali da eugenol primenjivan tokom dve i Äetiri nedelje u dozama
od 10, 50, 200 i 400 mg/kg tm/dan ne ispoljava hematotoksiÄno, hepatotoksiÄno niti
nefrotoksiÄno dejstvo. Eugenol davan tokom Äetiri nedelje ne utiÄe znaÄajno na broj
eritrocita, koncentraciju hemoglobina, hematokrit, zapreminu eritrocita, broj
leukocita i leukocitnu formulu. Primenjivan tokom dve nedelje, prouzrokuje znaÄajno
poveÄanje mase hemoglobina po eritrocitu. Ovaj efekat je nespecifiÄan i ne zavisi niti
doze, niti od dužine tretmana. Doze eugenola od 10 i 200 mg/kg/dan primenjivane
tokom Äetiri nedelje prouzrokuju znaÄajno smanjenje broja trombocita, dok najviÅ”a
ispitivana doza (400 mg/kg/dan) prouzrokuje znaÄajno poveÄanje, u poreÄenju sa
brojem trombocita kod pacova tretiranih dozom od 10 i 200 mg/kg/dan. Promene
broja trombocita izazvane eugenolom kvalitativno su razliÄite i ne zavise od doze, niti
od dužine tretmana. Eugenol primenjivan tokom dve i Äetiri nedelje ne utiÄe znaÄajno na proteinemiju, albuminemiju, koncentraciju uree i kreatinina, kao i aktivnost ALP u
serumu pacova.
Tri ispitivane doze eugenola (10, 50 i 200 mg/kg/dan) znaÄajno smanjuju, dok
najviÅ”a doza poveÄava aktivnost ALT. Eugenol aplikovan tokom Äetiri nedelje u dozi
od 200 i 400 mg/kg prouzrokuje statistiÄki znaÄajno smanjenje aktivnosti CK.The possible haematotoxic, hepatotoxic and nephrotoxic effects of eugenol in
rats after two-week and the four-week continuous use were tested. The experiment
was conducted on 72 male Wistar rats divided into six equal groups. Four were
treated with different doses of eugenol (10 mg/kg bw/day, 50 mg/kg/d, 200 mg/kg
bw/d and 400 mg/kg bw/d), the control group received the vehikulum (0.5%
methyl cellulose, 20% propylene glycol and water), and the sixth group was the
absolute, untreated control. Eugenol and the vehikulum were administered with a
gastric probe, on a daily basis, for four weeks at a rate of 1 ml/100 g body weight of
rats. Blood was collected by cardiac puncture of the anesthetised rats on days 14
and 28 in order to assess the haematological and biochemical blood parameters:
haematocrit, red blood cell count, white blood cell and platelet counts, leukocyte
formula, hemoglobin, MCV, MCH, total protein concentration, albumin, urea,
creatinine, and the activity of the alanine aminotransferase (ALT), alkaline
phosphatase (ALP) and creatine kinase (CK).
The results showed that, having been applied for two and four weeks at doses of 10,
50, 200 and 400 mg/kg bw/day, eugenol did not show any haematotoxic, hepatotoxic
or nephrotoxic effects. Eugenol administered for four weeks does not significantly
affect the number of erythrocytes, haemoglobin concentration, haematocrit, red blood cell volume, leukocyte count and leukocyte formula. Applied for two weeks it caused a
significant increase in the mass of haemoglobin in erythrocytes. This effect is not
specific and does not depend on the dose or the treatment duration. Eugenol in doses
of 10 and 200 mg/kg/day applied for four weeks caused a significant reduction in the
number of platelets, whilst the highest dose tested (400 mg/kg/day) caused a
significant increase in comparison to the number of platelets in rats treated with 10
and 200 mg/kg/day. Changes in the number of platelets induced by eugenol
qualitatively different and are not related to dose or length of treatment.
Eugenol applied for two and four weeks does not significantly affect proteinaemia,
albuminaemia, serum urea and creatinine concentrations, and serum ALP
activity in rats. Three assessed doses of eugenol (10, 50 and 200 mg/kg/d)
significantly decreased, whereas the highest dose increased the ALT activity.
However, eugenol applied for four weeks at a dose of 200 and 400 mg/kg/d caused
a statistically significant decrease in CK activity
STUDENTSā INJURIES AT THE FACULTY OF SPORT AND PHYSICAL EDUCATION - AN ANALYSIS OF TWO STUDY PROGRAMS
The aim of this research is to analyze the injuries of the students of the Faculty of Sport and Physical Education, as well as to determine, if possible, if the increase in the number of injuries is caused by increased pressure, brought by a new study program, when doing exercises. The sample was composed of 207 participants, who attended the Faculty of Sport and Physical Education, University of Nis, aged 23 years Ā± 6 months. The questionnaire was the main measuring instrument for the evaluation of injuries. The participants were asked to answer by circling options concerning the location of their injury, the classes during which the injury occurred, as well as the type of injury. All the gathered data have been represented numerically and in percentages, and used for further analysis. The analysis of the gathered results showed that there is a higher index of injury during practice with students who have studied according to the Undergraduate academic study program, and that there is a difference in the rate of injuries in certain classes between the two study programs. The results of this study show that an increase in the intensity of work and increase in the teaching hours led to higher risk of injury. Future research, dealing with similar topics, on the basis of this research may compare studentsā injuries in both the old and new school system, which gives this study a special significance
Odnos izmeÄu totalnog kapaciteta za vezivanje gvožÄa i koncentracije transferina kod novoroÄene prasadi tretirane gvožÄe-dekstranom
Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment.OdreÄivanje statusa gvožÄa u organizmu jedinke posle primene odreÄenog preparata ovog mikroelementa moguÄe je utvrditi odreÄivanjem njegove koncentracije u serumu i zasiÄenja transferina (Trf) gvožÄem. U kliniÄkoj i eksperimentalnoj praksi složeno odreÄivanje koncentracije Trf zamenjeno je jednostavnim odreÄ ivanjem ukupnog serumskog kapaciteta za vezivanje gvožÄa (TIBC). Cilj ovog rada je bio da se na modelu porasta serumskog Fe po i.m. aplikaciji Fe-dextrana novoroÄenoj prasadi, utvrdi odnos TIBC i Trf po aplikaciji ovog mikroelementa, kako bi se utvrdilo da li visoke doze gvožÄa u serumu utiÄu na vrednost TIBC kao mere za odreÄivanje koncentracije Trf. Vrednosti za serumsko Fe, TIBC i Trf poreÄene su izmeÄu grupe životinja koja je odmah po roÄenju dobila 150 mg Fe-dextrana i kontrolne grupe u kojoj životoinje nisu tretirane Fe-dextranom. Krv je uzorkovana pre aplikacije Fe-dextrana, drugog, osmog i dvanaestog dana po aplikaciji preparata gvožÄa. Koncentracija gvožÄa u serumu i TIBC su odreÄivani standarnim kliniÄkim biohemijskim analizama, dok je koncenracija Trf odreÄena denzitometrijom elektroforetskih traka. Dobijeni rezultati ukazuju da bez obzira na primenu preparata Fe, TIBC nije adekvatna mera za Trf kod novoroÄene prasadi u prva tri dana po roÄenju. Osmog dana života prasadi, utvrÄen je visoki stepen korelacije ova dva parametra kod životinja koje nisu bile tretirane, dok je kod tretiranih jedinki taj stepen korelacije postignut dvanaestog dana. Na osnovu izloženih rezultata se može zakljuÄiti da se kod novoroÄene prasadi TIBC može koristiti kao mera za Trf tek 12 dana nakon i.m. tretmana Fe-dextranom
UTICAJ PRODUŽENE PERORALNE PRIMENE EUGENOLA NA HEMATOLOŠKE I NEKE BIOHEMIJSKE PARAMETRE KRVI KOD PACOVA
Ispitivano je moguÄe hematotoksiÄno, hepatotoksiÄno i nefrotoksiÄano dejstvo eugenolakod pacova posle dvonedeljne i Äetvoronedeljne kontinuirane p.o. primene.Ogled je izveden na 72 mužjaka pacova soja Wistar podeljenih u Å”est grupa. Äetirigrupe tretirane su razliÄitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg tm/dan,200 mg/kg tm/dan i 400 mg/kg tm/dan). Peta, kontrolna grupa dobijala je vehikulum(0,5% metil-celuloza, 20% propilen-glikol i voda), a Å”esta je bila apsolutna, netretiranakontrola. Eugenol i vehikulum aplikovani su gastriÄnom sondom, svakodnevno tokomÄetiri nedelje u koliÄini od 1 ml/100 g telesne mase pacova. Krv je uzorkovanakardijalnom punkcijom anestetisanih pacova 14. i 28. dana ogleda u cilju odreÄivanjahematoloÅ”kih i biohemijskih parametara krvi: hematokrit, broj eritrocita, leukocita itrombocita, leukocitna formula, koncentracija hemoglobina, MCV, MCH, koncentracijaproteina, albumina, uree, kreatinina, kao i aktivnost alanin-aminotransferaze (ALT),alkalne fosfataze (ALP) i kreatin-kinaze (CK).Rezultati su pokazali da eugenol primenjivan tokom dve i Äetiri nedelje u dozamaod 10, 50, 200 i 400 mg/kg tm/dan ne ispoljava hematotoksiÄno, hepatotoksiÄno nitinefrotoksiÄno dejstvo. Eugenol davan tokom Äetiri nedelje ne utiÄe znaÄajno na brojeritrocita, koncentraciju hemoglobina, hematokrit, zapreminu eritrocita, brojleukocita i leukocitnu formulu. Primenjivan tokom dve nedelje, prouzrokuje znaÄajnopoveÄanje mase hemoglobina po eritrocitu. Ovaj efekat je nespecifiÄan i ne zavisi nitidoze, niti od dužine tretmana. Doze eugenola od 10 i 200 mg/kg/dan primenjivanetokom Äetiri nedelje prouzrokuju znaÄajno smanjenje broja trombocita, dok najviÅ”aispitivana doza (400 mg/kg/dan) prouzrokuje znaÄajno poveÄanje, u poreÄenju sabrojem trombocita kod pacova tretiranih dozom od 10 i 200 mg/kg/dan. Promenebroja trombocita izazvane eugenolom kvalitativno su razliÄite i ne zavise od doze, nitiod dužine tretmana. Eugenol primenjivan tokom dve i Äetiri nedelje ne utiÄe znaÄajnona proteinemiju, albuminemiju, koncentraciju uree i kreatinina, kao i aktivnost ALP userumu pacova.Tri ispitivane doze eugenola (10, 50 i 200 mg/kg/dan) znaÄajno smanjuju, doknajviÅ”a doza poveÄava aktivnost ALT. Eugenol aplikovan tokom Äetiri nedelje u doziod 200 i 400 mg/kg prouzrokuje statistiÄki znaÄajno smanjenje aktivnosti CK
Uticaj eugenola na hematoloŔke parametre kod pacova
Investigations covered the possible hematotoxic effect of eugenol in rats following two-week and four-week continuous p.o. application. An experiment was conducted on 72 maleWistar rats divided into six groups. Four groups were treated with different doses of eugenol (10 mg/kg bm/day, 50 mg/kg/day, 200 mg/kg/day and 400 mg/kg bm/day), the control group was administered a vehiculum (0,5 % methylcellulose, 20 % propylene glycol and water), and the sixth group was the absolute untreated control. Eugenol and the vehiculum were administered using a gastric probe in a volume of 1 ml/100 g body mass of rat. Blood was sampled using cardiac puncture on days 14 and 28 of the experiment in order to determine hematological parameters (hematocrit, number of erythrocytes, MCV, haemoglobin concentration, MCH, number of leukocytes, leukocyte formula, and number of thrombocytes). The results have shown that eugenol administered over 14 and 28 days in doses of 10, 50, 200 and 400 mg/kg bm/day has no hemolytic activity. Furthermore, administered over four weeks, it does not significantly affect the number of erythrocytes, haemoglobin concentration, hematocrit, erythrocyte volume, number of leukocytes, and the leukocyte formula. Applied over two weeks, eugenol causes a significant increase in the mass of haemoglobin per erythrocyte, in comparison with controls. This effect is non-specific and does not depend on the dose or on the duration of treatment. The eugenol doses of 10 and 200 mg/kg/day administered over a period of four weeks result in a statistically significant reduction in the number of thrombocytes in comparison with the absolute control, while the highest investigated dose (400 mg/kg) causes a significant increase in comparison with the numbers for rats treated with a dose of 10 and 200 mg/kg/day. The changes in thrombocyte number caused by eugenol are qualitatively different and depend neither on the dose nor on the duration of treatment. Eugenol applied over two or four weeks does not exhibit a significant influence on the number of lymphocytes, monocytes or granulocytes.Ispitivan je moguÄi hematotoksiÄni efekat eugenola kod pacova posle dvonedeljne i Äetvoronedeljne kontinuirane p.o. primene. Ogled je izveden na 72 mužjaka pacova soja vistar podeljenih u Å”est grupa. Äetiri grupe tretirane su razliÄitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg/dan, 200 mg/kg/dan i 400 mg/kg tm/dan), peta kontrolna grupa dobijala je vehikulum (0,5 % metil-celuloza, 20 % propilen-glikol i voda), a Å”esta je bila netretirana, apsolutno kontrolna. Eugenol i vehikulum aplikovani su gastriÄnom sondom u volumenu od 1 ml/100 g telesne mase pacova. Krv je uzorkovana kardijalnom punkcijom 14. i 28. dana ogleda u cilju odreÄivanja hematoloÅ”kih parametara (hematokrit, broj eritrocita, proseÄna vrednost zapremine eritrocita (MCV), koncentracija hemoglobina, proseÄna vrednost koncentracije hemoglobina (MCH), broj leukocita, leukocitna formula i broj trombocita). Rezultati su pokazali da eugenol primenjivan 14 i 28 dana u dozama od 10, 50, 200 i 400 mg/kg tm/dan nema hemolitiÄku aktivnost. TakoÄe, primenjivan tokom Äetiri nedelje ne utiÄe znaÄajno na broj eritrocita, koncentraciju hemoglobina, hematokrit, zapreminu eritrocita, broj leukocita i leukocitnu formulu. Eugenol primenjivan tokom dve nedelje prouzrokuje znaÄajno poveÄanje mase hemoglobina po eritrocitu u odnosu na kontrolu. Ovaj efekat je nespecifiÄan i ne zavisi od doze, ni od dužine tretmana. Doze eugenola od 10 i 200 mg/kg/dan primenjivane tokom Äetiri nedelje prouzrokuju statistiÄki znaÄajno smanjenje broja trombocita u odnosu na apsolutno kontrolnu grupu, dok najviÅ”a ispitivana doza (400 mg/kg) izaziva znaÄajno poveÄanje broja trombocita u poreÄenju sa dozama od 10 i 200 mg/kg/dan. Promene broja trombocita izazvane eugenolom kvalitativno su razliÄite i ne zavise niti od doze niti od dužine tretmana. Eugenol primenjivan tokom dve i Äetiri nedelje ne ispoljava znaÄajan uticaj na broj limfocita, monocita i granulocita
STATE NUTRITION THE YOUNGER ELEMENTARY SCHOOLCHILDREN
The obesty is a state characterized by pathological accumulation of fat tissue in the body, and nutritional disorders are associated with the high risk of numerous health problems since earliest childhood. The obesty has been increasing and it has now reached the proportion of an epidemic with the tendency of growth in the number of fat people. Such a negative trend directly influences the reduction of the functional and motorical abilities of the entire population. The research purpose to examine state nutrition the younger elementary schoolchildren and to see if there are deviations between the age groups. In the reasearching were included 313 tested divaidedin four groups. All tested were a regular pupils of the first and second grade of elementary school. In the resarching there were include the bouth the poles of pupils and the conditiones for testing were optimal. On the basis of anthropometric measurement of the body weight and height, body mass index (BMI) values were calculated. And if is index of body mass is possible to find a lot of critrcizm, where is the most important that is not support constructive specific of the one man, but it gave information with results is possible standardation and clasification. The differences have been established through multivariant analysis of MANOVA variance, single variant univariant analysis of ANOVA variance and Discriminative analyses
THE INFLUENCE OF THE EXPERIMENTAL PROGRAMME OF PHYSICAL EDUCATION CURRICULUM ON PUPILS MORPHOLOGICAL CARACTERISTICS
The purpose of this research is to determine how much is experimental programme, which was conducted duriing one school year, influenting the morphological caracteristics. The applied experimental program was composed of athletics exercises, sports games, exercises on the pieces of equipment and on the floor, rhythmics and dancing exercises and some additional exercises. 13 metrical instruments were used for evaluation of morphological caracteristics of schoolboys and schoolgirls. Research was conducted on 106 first-grade pupils of the elementary school, age of 7 years Ā± 6 months. Basic statistic parametars were calculated by processing of data during initial and final measuring. Final data processing included only the examinees that participated at initial and final measurement. Multivariant and univariant analisys of variance for repeated measures (MANOVA and ANOVA ā repeated measures) were applied for determination of eventual differences between initial and final measuring. Based on retrieved results it could be concluded that experimental programme has a positive influence on changes of some morphological caracteristics. The results of this paper can be used by PE teachers giving them the information on adequate planning and programming of the classes
THE INFLUENCE OF THE EXPERIMENTAL PROGRAMME OF PHYSICAL EDUCATION CURRICULUM ON PUPILS FUNCTIONAL ABILITIES
The purpose of this research is to determine how much is the specially suggested experimental program of physical education, with increased demands and application of additional exercises, influenting the functional abbilities. The applied experimental program was composed of athletics exercises, sports games, exercises on the pieces of equipment and on the floor, rhythmics and dancing exercises. Six metrical instruments were used for evaluation of functional abbilities of schoolboys and schoolgirls. The experimental program was carried out on the sample of 106 first-grade pupils of the elementary school, age of 7 years Ā± 6 months. Basic statistic parametars were calculated by processing of data during initial and final measuring. Final data processing included only the examinees that participated at initial and final measurement. Multivariant and univariant analisys of variance for repeated measures (MANOVA and ANOVA ā repeated measures) were applied for determination of eventual differences between initial and final measuring. Based on retrieved results it could be concluded that specially programed performance of physical education had a significant influence on changing the most functional abbilities of pupils