Sale Of Residence In Trust: Is The Exclusion Available?

With more use of trusts, particularly revocable inter vivos trusts, the question is being raised with increasing frequency as to whether sale of the residence by the trust is eligible for the $250,000 exclusion from income ($500,000 for married taxpayers) on a joint return. The stakes are high and may influence whether a residence is placed in trust

Data of infectivity assays

Data of infectivity assays for each host species (Trichoplusia ni and Spodoptera exigua). Columns represent ‘experiment’ (experiment number; two replicates per host species), ‘treatment’ (infection with AcMNPV WT (0) or AcMNPV Δegt (1)), ‘virus concentration’ (log occlusion bodies per ml), ‘N’ (number of larvae infected), and ‘dead larvae’ (number of larvae that died of virus infection)

raw_data_Fig2

Height (mm) per individual larva measured at different time points after mock-infection, from 0 hours post mock-infection until 214 hpi (light/dark) or 184 hpi (only dark).Two treatments: 1. mock-infected larvae kept under normal light/dark conditions; 2. mock-infected larvae kept under dark conditions

Challenges associated with CRISPR-Cas antimicrobials and potential routes to overcome them.

<p>A summary of the obstacles associated with using CRISPR-Cas–based antimicrobials in complex environmental populations of bacteria is shown. These include ensuring effective delivery of constructs (top left), routes of resistance evolution to these novel antimicrobials (top right), the species diversity and spatial complexity of bacterial communities (bottom left), and uncertainty in usage guidelines and stakeholder support (bottom right). AMR, antimicrobial resistance; CRISPR-Cas, clustered regularly interspaced short palindromic repeats-CRISPR–associated.</p

raw data Figure 1C

Height (mm) at which each individual larva (n=16) was found at the indicated time points. At each time point survival was monitored (alive/dead =A/D)

raw_data_Fig1D

Height (mm) at which each individual larva (n=32) was found at the indicated time points. At each time point survival was monitored (alive/dead =A/D)

raw data Figure 1B

Height (mm) at which each individual larva (n=16) was found at the indicated time points . At each time point survival was monitored (alive/dead =A/D)

The <i>ptp</i> gene is present in all Alphabaculovirus group I NPVs.

<p>Bayesian phylogeny of baculoviruses based on the <i>lef-8</i> gene. GenBank accession numbers are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046933#pone.0046933.s003" target="_blank">Table S3</a>. Numbers in bold indicate maximum likelihood bootstrap values based on 100 replicates, while plain numbers depict Bayesian posterior probabilities. Only values ≥50 are indicated for both analyses. The bar at the bottom indicates a branch length of 10% distance. Baculoviruses possessing a <i>ptp</i> gene are marked by a black dot, while baculoviruses possessing a <i>ptp2</i> gene are marked by a black diamond.</p

Variation between different replicates in movement assays of mock- and WT-infected larvae at one, two and three dpi.

<p>Minimum, maximum and mean (± SE) distances moved of all replicates are displayed.</p

The baculovirus <i>ptp</i> gene was presumably acquired from a lepidopteran host by an ancestral NPV.

<p>Bayesian phylogeny of <i>ptp</i> nucleotide sequences from diverse invertebrate taxa. GenBank and Butterflybase/InsectaCentral accession numbers are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046933#pone.0046933.s002" target="_blank">Table S2</a>. Numbers in bold indicate maximum likelihood bootstrap values based on 100 replicates, while plain numbers depict Bayesian posterior probabilities. Only values ≥50 are indicated for both analyses. The bar at the bottom indicates a branch length of 10% distance.</p