The importance of wild resources as a reflection of the resilience and changing nature of early agricultural systems in East Asia and Europe

We examine the changing importance of wild starch rich plant staples, predominantly tree nuts, in early agricultural societies in East Asia and Europe, focusing on Korea, Japan, and Britain. A comparative review highlights variations in the importance of wild plant staples compared to domesticated crops. The Korean Middle to Late Chulmun periods (c. 3,500–1,500 BC) was characterized by a high reliance on nuts alongside millet. This declines with the transition to rice agriculture, but remains significant during the Mumun period (c. 1,500–300 BC). In Japan, the arrival of rice and millets in the Yayoi Period (c. 1,000 BC−250 AD) saw continued evidence for high levels of reliance on wild resources, which declines only in the Kofun and early historical periods. In Early Neolithic Britain (c. 4,000–3,300 BC) cereal agriculture is accompanied by high evidence for wild plant foods. But during the Middle to Late Neolithic (3,300–c. 2,400/2,200 BC) cereals were abandoned on the mainland with hazelnuts becoming a prominent plant staple. Agriculture returned in the second half of the 3rd millennium BC, followed by a strong decline in wild plant food use during the Middle to Late Bronze Age (1,700–700 BC). Such patterns have previously been attributed to the slow adoption of farming by indigenous peoples, with a continued reliance on wild resources. In light of evidence demonstrating that the dispersal of agriculture was largely driven by a mixture of demic-diffusion and introgression of hunter-gatherers into agricultural groups, a reinterpretation of the role of wild foods is needed. It is argued that the relative importance of wild plant staples provides an indicator of the stability and dependability of agricultural and social systems. A heavy reliance on wild foods in early agricultural societies is tied to the slow adaptation of domesticated crops to new environments, where agricultural and social landscapes are yet to be firmly established, and social systems that could mitigate for poor harvests and storage were often absent. The retained lengthy persistence of wild plant staples in East Asian subsistence systems compared to the British Isles likely reflects differences in the ecological and labor demands for rice compared to Western Asiatic cereals

Ex-vivo perfusion bioassay : an excellent technique to measure the bioactivity of inhalable insulin coated microcrystals

Purpose: To measure the bioactivity of inhalable insulin coated microcrystals using a perfusion bioassay that measures its vasodilatory effect on smooth muscle arterial tissue. Methods: The bioactivity of an insulin protein coated microcrystal (PCMC), a potential candidate for pulmonary drug delivery and commercial insulin was determined on a Danish Myo Tech P110 pressure myograph system. 12 week old Mesenteric resistance arteries from Male Wistar rats were isolated and immersed in a physiological salt solution (PSS) and attached to 2 opposing hollow glass micro-cannula (outer diameter 80 microns). The PSS was gradually warmed to 37°C (at a pressure less than 5mm Hg) for 1hr. Subsequently the pressure was increased up to 40mm Hg over a period 15 minutes and equilibrated for a further 15 minutes after gassing with 95%O2 / 5%CO2 to achieve a pH of 7.4 at 37°C. After normalisation by two washes of 123mM KCl and exposure to 1-10mM noradrenaline the arteries were exposed intraluminally to each insulin preparation by gradual infusion directly into the lumen via a fetal microcannulae inserted to the tip of the glass mounting cannula, at a constant pressure. Results: The preliminary results (full cummulative response curve yet to be determined) demonstrate insulin mediated relaxation to noradrenaline preconstriction. The level of constriction drops from 100% to 42% as the concentration of insulin increases from -11 to -9 Log M for the PCMC compared with a drop from 100 % to 65% for the commercial insulin preparation. However the more potent vasodilatory effect found for the insulin PCMC is more likely to be a result of variance introduced in each dilution step than a real increase in potency. Conclusion: The perfusion bioassay technique provides an excellent method of measuring insulin bioactivity and indicates the insulin loaded on the microcrystal support is fully active

Dynamic range in the C.elegans brain network

We study external electrical perturbations and their responses in the brain dynamic network of the Caenorhabditis eleganssoil worm, given by the connectome of its large somatic nervous system. Our analysis is inspired by a realistic experiment where one stimulates externally specific parts of the brain and studies the persistent neural activity triggered in other cortical regions. In this work, we perturb groups of neurons that form communities, identified by the walktrap community detection method, by trains of stereotypical electrical Poissonian impulses and study the propagation of neural activity to other communities by measuring the corresponding dynamic ranges and Steven law exponents. We show that when one perturbs specific communities, keeping the rest unperturbed, the external stimulations are able to propagate to some of them but not to all. There are also perturbations that do not trigger any response. We found that this depends on the initially perturbed community. Finally, we relate our findings for the former cases with low neural synchronization, self-criticality, and large information flow capacity, and interpret them as the ability of the brainnetwork to respond to external perturbations when it works at criticality and its information flow capacity becomes maximal

An orbital perspective on the starvation, stripping, and quenching of satellite galaxies in the EAGLE simulations

Using the EAGLE suite of simulations, we demonstrate that both cold gas stripping {\it and} starvation of gas inflow play an important role in quenching satellite galaxies across a range of stellar and halo masses, $M_{\star}$ and $M_{200}$. By quantifying the balance between gas inflows, outflows, and star formation rates, we show that even at $z=2$, only $\approx30\%$ of satellite galaxies are able to maintain equilibrium or grow their reservoir of cool gas - compared to $\approx50\%$ of central galaxies at this redshift. We find that the number of orbits completed by a satellite is a very good predictor of its quenching, even more so than the time since infall. On average, we show that intermediate-mass satellites with $M_{\star}$ between $10^{9}{\rm M}_{\odot}-10^{10}{\rm M}_{\odot}$ will be quenched at first pericenter in massive group environments, $M_{200}>10^{13.5}{\rm M}_{\odot}$; and will be quenched at second pericenter in less massive group environments, $M_{200}<10^{13.5}{\rm M}_{\odot}$. On average, more massive satellites ($M_{\star}>10^{10}{\rm M}_{\odot}$) experience longer depletion time-scales, being quenched between first and second pericenters in massive groups; while in smaller group environments, just $\approx30\%$ will be quenched even after two orbits. Our results suggest that while starvation alone may be enough to slowly quench satellite galaxies, direct gas stripping, particularly at pericenters, is required to produce the short quenching time-scales exhibited in the simulation.Comment: Accepted for publication in MNRA

Analysis of host response to bacterial infection using error model based gene expression microarray experiments

A key step in the analysis of microarray data is the selection of genes that are differentially expressed. Ideally, such experiments should be properly replicated in order to infer both technical and biological variability, and the data should be subjected to rigorous hypothesis tests to identify the differentially expressed genes. However, in microarray experiments involving the analysis of very large numbers of biological samples, replication is not always practical. Therefore, there is a need for a method to select differentially expressed genes in a rational way from insufficiently replicated data. In this paper, we describe a simple method that uses bootstrapping to generate an error model from a replicated pilot study that can be used to identify differentially expressed genes in subsequent large-scale studies on the same platform, but in which there may be no replicated arrays. The method builds a stratified error model that includes array-to-array variability, feature-to-feature variability and the dependence of error on signal intensity. We apply this model to the characterization of the host response in a model of bacterial infection of human intestinal epithelial cells. We demonstrate the effectiveness of error model based microarray experiments and propose this as a general strategy for a microarray-based screening of large collections of biological samples

Emerging evidence of plant domestication as a landscape-level process

Current theories of plant domestication are based on localized founder models in which single or multiple domestications occur as a progressive result of adaptation processes, but anomalies that do not fit within this perspective have been accumulating. We describe developments in archaeology and genetics over the past decade in which cultural connections between groups stretch back much further in time than was previously realized, and over wide geographic distances. Weak selection for domestication substantially pre-dates domestication and/or cultivation practices, large populations appear to have been maintained throughout the emergence of domesticates, and the resulting forms were not necessarily an improvement in terms of yield. We present a framework in which the process of domestication evolved as a landscape-level process involving large populations connected through sustained long-term human contact over large distances from which domesticate forms emerged in a complex manner as an adaptive reaction to long-term exploitation that did not necessarily provide immediate benefits. The landscape framework addresses several anomalies and radically changes the dynamic visualization of the evolution of domestication. It also opens up a list of new questions regarding the mechanisms of selection and the assembly of domestication syndrome alleles, and obliges a profound rethink of the progressive nature of domestication and human cultural evolution. The evidence from ancient crops over the past decade challenges some of our most basic assumptions about the process of domestication. The emergence of crops has been viewed as a technologically progressive process in which single or multiple localized populations adapt to human environments in response to cultivation. By contrast, new genetic and archaeological evidence reveals a slow process that involved large populations over wide areas with unexpectedly sustained cultural connections in deep time. We review evidence that calls for a new landscape framework of crop origins. Evolutionary processes operate across vast distances of landscape and time, and the origins of domesticates are complex. The domestication bottleneck is a redundant concept and the progressive nature of domestication is in doubt

An Archaeological Radiocarbon Database of Japan

We present a radiocarbon database for the Japanese archipelago compiled from over 5,500 site excavation reports covering a chronological span from 55,000 BP to the present day. The complete database in Japanese contains over 44,000 entries, providing contextual information directly obtained from descriptions provided in the site reports. Here we provide a curated English translation of the database, containing a subset of 39,284 dates from the original database, which excludes duplicates and errors and includes new information concerning the dated material