Russian Women: Living in History’s Shadow

From the Essay: There has never been one “woman question” in Russia, but rather many. During an extended stay in St. Petersburg in the summer of 1999, we attended a conference on women’s issues and interviewed a number of women from all over Russia, trying to ascertain for ourselves the state of the “woman question” in post-Soviet Russia. In our conversations with women, it became clear that as some test the new economic and political waters, as others see their future in the traditions of the Orthodox church, and still others feel ill-positioned to succeed in the new Russia, the historical legacy of life under the Soviet system may prove the most difficult challenge for Russian women to overcome. Our discussions did not provide an answer, but we hope that what we learned can add some complexity and nuance to how the question is asked

The Present in the Past: Ohio State’s website

Histor

The SUMO Ligase Protein Inhibitor of Activated STAT 1 (PIAS1) is a constituent PML-NB protein that contributes to the intrinsic antiviral immune response to herpes simplex virus 1 (HSV-1)

Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukaemia (PML)-nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting viral genomes to cooperatively promote viral genome silencing. Central to the execution of this particular antiviral response is the small ubiquitin-like modifier (SUMO) signalling pathway. However, the participating SUMOylation enzymes are not fully characterized. We identify the SUMO ligase Protein Inhibitor of Activated STAT1 (PIAS1) as a constituent PML-NB protein. We show that PIAS1 localizes at PML-NBs in a SUMO interaction motif (SIM)-dependent manner that requires SUMOylated or SUMOylation competent PML. Following infection with herpes simplex virus 1 (HSV-1), PIAS1 is recruited to nuclear sites associated with viral genome entry in a SIM-dependent manner, consistent with the SIM-dependent recruitment mechanisms of other well characterized PML-NB proteins. In contrast to Daxx and Sp100, however, the recruitment of PIAS1 is enhanced by PML. PIAS1 promotes the stable accumulation of SUMO1 at nuclear sites associated with HSV-1 genome entry, whereas the accumulation of other evaluated PML-NB proteins occurs independently of PIAS1. We show that PIAS1 cooperatively contributes to HSV-1 restriction through mechanisms that are additive to those of PML and cooperative with those of PIAS4. The antiviral mechanisms of PIAS1 are counteracted by ICP0, the HSV-1 SUMO-targeted ubiquitin ligase, which disrupts the recruitment of PIAS1 to nuclear domains that contain infecting HSV-1 genomes through mechanisms that do not directly result in PIAS1 degradation

Variable stars in the field of the Hydra II ultra-faint dwarf galaxy

We report the discovery of one RR Lyrae star in the ultra--faint satellite galaxy Hydra II based on time series photometry in the g, r and i bands obtained with the Dark Energy Camera at Cerro Tololo Interamerican Observatory, Chile. The RR Lyrae star has a mean magnitude of $i = 21.30\pm 0.04$ which translates to a heliocentric distance of $151\pm 8$ kpc for Hydra II; this value is $\sim 13\%$ larger than the estimate from the discovery paper based on the average magnitude of several blue horizontal branch star candidates. The new distance implies a slightly larger half-light radius of $76^{+12}_{-10}$ pc and a brighter absolute magnitude of $M_V = -5.1 \pm 0.3$, which keeps this object within the realm of the dwarf galaxies. The pulsational properties of the RR Lyrae star ($P=0.645$ d, $\Delta g = 0.68$ mag) suggest Hydra II may be a member of the intermediate Oosterhoff or Oosterhoff II group. A comparison with other RR Lyrae stars in ultra--faint systems indicates similar pulsational properties among them, which are different to those found among halo field stars and those in the largest of the Milky Way satellites. We also report the discovery of 31 additional short period variables in the field of view (RR Lyrae, SX Phe, eclipsing binaries, and a likely anomalous cepheid). However, given their magnitudes and large angular separation from Hydra II, they must be field stars not related to Hydra II.Comment: Revised version after comments from the referee. Accepted for publication in A

Altruism, Commitment, and Leadership in High School Mentors

This study investigated the effects of mentoring on selected attributes among high school mentors. Three attributes were explored: altruism, commitment to school, and student leadership. Seventy-four high school juniors and seniors participated as mentors to high school freshmen students. Mentors participated in a leadership training program prior to beginning their mentoring activities. Pre- and post-test measures of the three attributes were administered. Results showed no significant increase in altruism or commitment scores. Unexpectedly, the mentoring experience produced a significant decrease in the perception of leadership scores. Implications for implementing a mentoring program in a high school setting are discussed

Altruism, Commitment, and Leadership in High School Mentors

This study investigated the effects of mentoring on selected attributes among high school mentors. Three attributes were explored: altruism, commitment to school, and student leadership. Seventy-four high school juniors and seniors participated as mentors to high school freshmen students. Mentors participated in a leadership training program prior to beginning their mentoring activities. Pre- and post-test measures of the three attributes were administered. Results showed no significant increase in altruism or commitment scores. Unexpectedly, the mentoring experience produced a significant decrease in the perception of leadership scores. Implications for implementing a mentoring program in a high school setting are discussed

Koinonia

Spotlight FeaturesSovereign Stumbling: My Life Journey to Date, Larry Crabb Conversations About Racism, Jessie Brown Anxiety: A Growing Problem in College Students, Steven M. Conn Thinking TheologicallyTeaching the Truth, Michael and Stephanie Santarosa Book ReviewsKingdom Triangle: Recover the Christian Mind, Renovate the Soul, Restore the Spirit\u27s Power (by J.P. Moreland), reviewed by Steve Ivester The Soul of a Christian University: A Field Guide for Educators (edited by Stephen T. Beers), reviewed by Kyle Lantz The Outrageous Idea of Academic Faithfullness (by Donald Opitz and Derek Melleby), reviewed by Nathan Geer I Once Was Lost: What Postmodern Skeptics Taught Us About Their Path to Jesus (by Don Everts and Doug Schaupp), reviewed by Andrew D. Rowell FeaturesThe President\u27s Corner Editor\u27s Deskhttps://pillars.taylor.edu/acsd_koinonia/1012/thumbnail.jp

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Background: Management of influenza, a major contributor to the worldwide disease burden, is complicated by lack of reliable methods for early identification of susceptible individuals. Identification of molecular markers that can augment existing diagnostic tools for prediction of severity can be expected to greatly improve disease management capabilities. Methodology/Principal Findings: We have analyzed cytokines, proteome flux and protein adducts in bronchoalveolar lavage (BAL) and sera from mice infected with influenza A virus (PR8 strain) using a previously established non-lethal model of influenza infection. Through detailed cytokine and protein adduct measurements of murine BAL, we first established the temporal profile of innate and adaptive responses as well as macrophage and neutrophil activities in response to influenza infection. A similar analysis was also performed with sera from a longitudinal cohort of influenza patients. We then used an iTRAQ-based, comparative serum proteome analysis to catalog the proteome flux in the murine BAL during the stages correlating with “peak viremia,” “inflammatory damage,” as well as the “recovery phase.” In addition to activation of acute phase responses, a distinct class of lung proteins including surfactant proteins was found to be depleted from the BAL coincident with their “appearance” in the serum, presumably due to leakage of the protein following loss of the integrity of the lung/epithelial barrier. Serum levels of at least two of these proteins were elevated in influenza patients during the febrile phase of infection compared to healthy controls or to the same patients at convalescence. Conclusions/Significance: The findings from this study provide a molecular description of disease progression in a mouse model of influenza and demonstrate its potential for translation into a novel class of markers for measurement of acute lung injury and improved case management.Singapore. National Research FoundationSingapore-MIT Alliance for Research and Technology (ID-IRG research program

In Vitro Pharmacological Characterization of RXFP3 Allosterism: An Example of Probe Dependency

Recent findings suggest that the relaxin-3 neural network may represent a new ascending arousal pathway able to modulate a range of neural circuits including those affecting circadian rhythm and sleep/wake states, spatial and emotional memory, motivation and reward, the response to stress, and feeding and metabolism. Therefore, the relaxin-3 receptor (RXFP3) is a potential therapeutic target for the treatment of various CNS diseases. Here we describe a novel selective RXFP3 receptor positive allosteric modulator (PAM), 3-[3,5-Bis(trifluoromethyl)phenyl]-1-(3,4-dichlorobenzyl)-1-[2-(5-methoxy-1H-indol-3-yl)ethyl]urea (135PAM1). Calcium mobilization and cAMP accumulation assays in cell lines expressing the cloned human RXFP3 receptor show the compound does not directly activate RXFP3 receptor but increases functional responses to amidated relaxin-3 or R3/I5, a chimera of the INSL5 A chain and the Relaxin-3 B chain. 135PAM1 increases calcium mobilization in the presence of relaxin-3NH2 and R3/I5NH2 with pEC50 values of 6.54 (6.46 to 6.64) and 6.07 (5.94 to 6.20), respectively. In the cAMP accumulation assay, 135PAM1 inhibits the CRE response to forskolin with a pIC50 of 6.12 (5.98 to 6.27) in the presence of a probe (10 nM) concentration of relaxin-3NH2. 135PAM1 does not compete for binding with the orthosteric radioligand, [125I] R3I5 (amide), in membranes prepared from cells expressing the cloned human RXFP3 receptor. 135PAM1 is selective for RXFP3 over RXFP4, which also responds to relaxin-3. However, when using the free acid (native) form of relaxin-3 or R3/I5, 135PAM1 doesn't activate RXFP3 indicating that the compound's effect is probe dependent. Thus one can exchange the entire A-chain of the probe peptide while retaining PAM activity, but the state of the probe's c-terminus is crucial to allosteric activity of the PAM. These data demonstrate the existence of an allosteric site for modulation of this GPCR as well as the subtlety of changes in probe molecules that can affect allosteric modulation of RXFP3