Water ascends in woody plants : so what?

Woody plants are often considered as static individuals, taking up water via the root system and losing it again via leaf transpiration. Quite boring one might conclude when considering that more than 95% of the water taken up by the roots is transpired by the leaves. But the story suddenly becomes much more intriguing when the dynamics happening during the ascent of water in woody plants are included. This keynote talk will elaborate on water transport in woody plants, highlighting where the dynamics come from, what the relevance is and which tissues are involved. The power to decipher water transport dynamics with plant-based measurements, where sap flow and stem diameter variations are at the forefront, will be illustrated. The concept of plant-based measurements will be complemented with a viewpoint on how sophisticated mechanistic water transport models can assist in plant-based irrigation scheduling or early warning and stress detection systems

Long-term observations of Uranus and Neptune at 90 GHz with the IRAM 30m telescope - (1985 -- 2005)

The planets Uranus and Neptune with small apparent diameters are primary calibration standards. We investigate their variability at ~90 GHz using archived data taken at the IRAM 30m telescope during the 20 years period 1985 to 2005. We calibrate the planetary observations against non-variable secondary standards (NGC7027, NGC7538, W3OH, K3-50A) observed almost simultaneously. Between 1985 and 2005, the viewing angle of Uranus changed from south-pole to equatorial. We find that the disk brightness temperature declines by almost 10% (~2sigma) over this time span indicating that the south-pole region is significantly brighter than average. Our finding is consistent with recent long-term radio observations at 8.6 GHz by Klein & Hofstadter (2006). Both data sets do moreover show a rapid decrease of the Uranus brightness temperature during the year 1993, indicating a temporal, planetary scale change. We do not find indications for a variation of Neptune's brightness temperature at the 8% level. If Uranus is to be used as calibration source, and if accuracies better than 10% are required, the Uranus sub-earth point latitude needs to be taken into account.Comment: accepted for publication in A&

A Mariotte-based verification system for heat-based sap flow sensors

Determination of the accuracy of commonly used techniques for measuring sap flux density in trees often presents a challenge. We therefore designed and built a verification system for heat-based sap flow sensors typically used at stem level. In the laboratory, a Mariotte's bottle device was used to maintain a constant flow rate of water through freshly cut stem segments of American beech (Fagus grandifolia Ehrh.). This verification system was used to determine the accuracy of three heat-based sap flux density techniques: heat pulse velocity, thermal dissipation and heat field deformation. All three techniques substantially underestimated sap flux density when compared against gravimetric measurements. On average the actual sap flux density was underestimated by 35% using heat pulse velocity, 46% using heat field deformation and 60% using thermal dissipation. These differences were consistent across sap flux densities ranging from 5 to 80 cm(3) cm(-2) h(-1). Field measurements supported the relative sensor performance observed in the laboratory. Applying a sensor-specific correction factor based on the laboratory test to the field data produced similar estimates of sap flux density from all three techniques. We concluded that a species-specific calibration is therefore necessary when using any of these techniques to insure that accurate estimates of sap flux density are obtained, at least until a physical basis for error correction can be proposed

Putting two water transport models to the test under wet and dry conditions

In order to improve fruit quality and quantity, accurate monitoring of the water status is necessary. The water status can be continuously predicted by using a mechanistic water transport and storage model (e.g. Steppe et al., 2006; 2008). This model typically links measurements of sap flow rate (SF) and stem diameter variations (D) to simulate stem water potential (Ψstem), which is recognised as one of the best indicators for evaluating plant water status. Despite good model performance under sufficient water availability, the model fails under dry conditions. However, a proper simulation of water transport under drought is essential for many applications. For example, grapevines are often subjected to some level of drought stress during the growing season in order to improve the quality of the grapes. Therefore, we aim at adjusting the existing model to improve its performance in simulating water transport during drought conditions. First, a dynamic function describing changes in hydraulic xylem resistance is used to replace the former constant parameter, and represents the resistances encountered in the soil, root and stem (RX). Second, also the former constant radial flow resistance between xylem and storage tissues has been replaced by an equation (RS). For the first time, equations for RX and RS instead of parameters were used in the model, and simulations were compared to the original ones. Both models functioned well under wet conditions, but where the original model failed under dry events, the adapted model could still accurately simulate D and Ψstem under these conditions. The adapted model is thus capable of describing the grapevine’s hydraulic response to both wet and (severe) drought conditions and seems very promising within the context of an automatic plant-based system for water status monitoring

Understanding plant responses to drought: how important is woody tissue photosynthesis?

Within trees, it is known that a part of the respired CO2 is assimilated in chlorophyll-containing stem and branch tissues. However, the role of this woody tissue photosynthesis in tree functioning remains unclear, in particular under drought stress conditions. In this study, stem diameter and leaf photosynthesis were measured for one-year-old cutting-derived plants of Populus nigra 'Monviso' under both well-watered and drought stress conditions. Half of the plants were subjected to a stem and branch light-exclusion treatment to prevent woody tissue photosynthesis to occur, while the other trees served as controls. Drought stress was induced in both treatments by limiting the water supply. We found that under well-watered conditions, light-exclusion resulted in reduced stem radial daily growth rate (DG) relative to DG observed for control trees. In response to drought, stem shrinkage of the light-excluded trees was more pronounced as compared to the control trees. Maximum leaf net photosynthesis (A(max)) decreased more rapidly in light-excluded trees compared to the controls during drought stress. Our results are the first to report on the potentially significant role of woody tissue photosynthesis in tree drought stress tolerance. Moreover, our study implies that the impact of assimilation of respired CO2 on tree functioning extends beyond local stem processes and indicates that woody tissue photosynthesis is potentially a key factor in understanding plant responses to drought stress

Measurement of sap flow dynamics through the tomato peduncle using a non-invasive sensor based on the heat field deformation method

Recent contradicting evidence on the contributions of xylem and phloem to tomato fruit growth highlights the need for a more thorough insight into the dynamics of sap flow through the tomato peduncle. In fact, knowledge on sap flow dynamics through small plant parts remains scarce, due to a lack of direct measurements. Most currently available sap flow sensors use needles, making them inappropriate for the direct measurement of sap flow through small plant parts such as a tomato peduncle. Therefore, a non-invasive sap flow sensor based on the heat field deformation (HFD) principle was tested on the peduncle of a tomato truss. This mini HFD sensor, consisting of a heater element and three thermocouples stitched on insulation tape, was wrapped around the peduncle and allowed continuous monitoring of changes in the heat field around the heater caused by sap flow. Actual influx into the tomato truss was calculated based on fruit growth data and estimates of fruit transpiration and was compared with the dynamics measured with the mini HFD sensor. Additionally, heat girdling of the peduncle was performed to block phloem influx to study the dynamics of xylem and phloem influx using the mini HFD sensor. First results of the mini HFD sensor were promising and the measured sap flow dynamics through the tomato peduncle agreed well with the calculated sap influx. Results of the girdling experiment suggested opposite patterns of xylem and phloem influx, with a decreased xylem influx during the daytime. Furthermore, the pattern of xylem influx revealed a close relation with the total water potential in the stem. As such, the mini HFD sensor provided direct measurements of sap flow dynamics through a tomato peduncle and, hence, has a large potential to finally resolve the controversy on water influx into developing fruits

TreeWatch.net : a water and carbon monitoring and modeling network to assess instant tree hydraulics and carbon status

TreeWatch.net is an initiative that has been developed to watch trees grow and function in real-time. It is a water- and carbon-monitoring and modeling network, in which high quality measurements of sap flow and stem diameter variation are collected on individual trees. Automated data processing using a cloud service enables instant visualization of water movement and radial stem growth. This can be used to demonstrate the sensitivity of trees to changing weather conditions, such as drought, heat waves, or heavy rain showers. But TreeWatch.net's true innovation lies in its use of these high precision harmonized data to also parameterize process-based tree models in real-time, which makes displaying the much needed mechanisms underlying tree responses to climate change possible. Continuous simulation of turgor to describe growth processes and long-term time series of hydraulic resistance to assess drought-vulnerability in real-time are only a few of the opportunities our approach offers. TreeWatch.net has been developed with the view to be complementary to existing forest monitoring networks and with the aim to contribute to existing dynamic global vegetation models. It provides high-quality data and real-time simulations in order to advance research on the impact of climate change on the biological response of trees and forests. Besides its application in natural forests to answer climate-change related scientific and political questions, we also envision a broader societal application of TreeWatch.net by selecting trees in nature reserves, public areas, cities, university areas, schoolyards, and parks to teach youngsters and create public awareness on the effects of changing weather conditions on trees and forests in this era of climate change

Deciphering acoustic emission signals in drought stressed branches: the missing link between source and sensor

When drought occurs in plants, acoustic emission (AE) signals can be detected, but the actual causes of these signals are still unknown. By analyzing the waveforms of the measured signals, it should, however, be possible to trace the characteristics of the AE source and get information about the underlying physiological processes. A problem encountered during this analysis is that the waveform changes significantly from source to sensor and lack of knowledge on wave propagation impedes research progress made in this field. We used finite element modeling and the well-known pencil lead break source to investigate wave propagation in a branch. A cylindrical rod of polyvinyl chloride was first used to identify the theoretical propagation modes. Two wave propagation modes could be distinguished and we used the finite element model to interpret their behavior in terms of source position for both the PVC rod and a wooden rod. Both wave propagation modes were also identified in drying-induced signals from woody branches, and we used the obtained insights to provide recommendations for further AE research in plant science