Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion

Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that suppression of the outer layer COPII component, Sec13, in zebrafish embryos results in a disorganized gut epithelium. In human intestinal epithelial cells (Caco-2), Sec13 depletion causes defective epithelial polarity and organization on permeable supports. Defects are seen in the ability of cells to adhere to the substrate, form a monolayer and form intercellular junctions. When embedded in a three-dimensional matrix, Sec13-depleted Caco-2 cells form cysts but, unlike controls, are defective in lumen expansion. Incorporation of primary fibroblasts within the three-dimensional culture substantially restores normal morphogenesis. We conclude that efficient COPII-dependent secretion, notably assembly of Sec13–Sec31, is required to drive epithelial morphogenesis in both two- and three-dimensional cultures in vitro, as well as in vivo. Our results provide insight into the role of COPII in epithelial morphogenesis and have implications for the interpretation of epithelial polarity and organization assays in cell culture

Reinforced carbon-carbon oxidation behavior in convective and radiative environments

Reinforced carbon-carbon, which is used as thermal protection on the space shuttle orbiter wing leading edges and nose cap, was tested in both radiant and plasma arcjet heating test facilities. The test series was conducted at varying temperatures and pressures. Samples tested in the plasma arcjet facility had consistently higher mass loss than those samples tested in the radiant facility. A method using the mass loss data is suggested for predicting mission mass loss for specific locations on the Orbiter

An analytical analysis of the dispersion predictions for effluents from the Saturn 5 and Scout-Algol 3 rocket exhausts

Predictions of the spatial concentration mapping of the potentially toxic constituents of the exhaust effluents from a launch of a Saturn 5 and of a Scout-Algol 3 vehicle utilizing the NASA/MSFC Multilayer Diffusion Program are provided. In the case of the Saturn 5, special attention was given to the concentration fields of carbon monoxide with a correlation of carbon dioxide concentrations. The Scout-Algol 3 provided an example of the centerline concentrations of hydrogen chloride, carbon monoxide, and alumina under typical meteorological conditions. While these results define the specific environmental impact of these two launches under the meteorological conditions existing during launches, they also provide a basis for the empirical monitoring of the constituents of the exhaust effluents of these vehicles

Ride quality meter

A ride quality meter is disclosed that automatically transforms vibration and noise measurements into a single number index of passenger discomfort. The noise measurements are converted into a noise discomfort value. The vibrations are converted into single axis discomfort values which are then converted into a combined axis discomfort value. The combined axis discomfort value is corrected for time duration and then summed with the noise discomfort value to obtain a total discomfort value

Residents' annoyance responses to aircraft noise events

In a study conducted in the vicinity of Salt Lake City International Airport, community residents reported their annoyance with individual aircraft flyovers during rating sessions conducted in their homes. Annoyance ratings were obtained at different times of the day. Aircraft noise levels were measured, and other characteristics of the aircraft were noted by trained observers. Metrics commonly used for assessing aircraft noise were compared, but none performed significantly better than A-weighted sound pressure level. A significant difference was found between the ratings of commercial jet aircraft and general aviation propeller aircraft, with the latter being judged less annoying. After the effects of noise level were accounted for, no significant differences were found between the ratings of landings and takeoffs. Aircraft noise annoyance reactions are stronger in lowered ambient noise conditions. This is consistent with the theory that reduced nighttime and evening ambient levels could create different reactions at different times of day. After controlling for ambient noise in a multiple regression analysis, no significant differences were found between the ratings of single events obtained during the three time periods: morning, afternoon, and evenings

An Analytic Equation of State for Ising-like Models

Using an Environmentally Friendly Renormalization we derive, from an underlying field theory representation, a formal expression for the equation of state, $y=f(x)$, that exhibits all desired asymptotic and analyticity properties in the three limits $x\to 0$, $x\to \infty$ and $x\to -1$. The only necessary inputs are the Wilson functions $\gamma_\lambda$, $\gamma_\phi$ and $\gamma_{\phi^2}$, associated with a renormalization of the transverse vertex functions. These Wilson functions exhibit a crossover between the Wilson-Fisher fixed point and the fixed point that controls the coexistence curve. Restricting to the case N=1, we derive a one-loop equation of state for $2< d<4$ naturally parameterized by a ratio of non-linear scaling fields. For $d=3$ we show that a non-parameterized analytic form can be deduced. Various asymptotic amplitudes are calculated directly from the equation of state in all three asymptotic limits of interest and comparison made with known results. By positing a scaling form for the equation of state inspired by the one-loop result, but adjusted to fit the known values of the critical exponents, we obtain better agreement with known asymptotic amplitudes.Comment: 10 pages, 2 figure

Materials technology assessment for stirling engines

A materials technology assessment of high temperature components in the improved (metal) and advanced (ceramic) Stirling engines was undertaken to evaluate the current state-of-the-art of metals and ceramics, identify materials research and development required to support the development of automotive Stirling engines, and to recommend materials technology programs to assure material readiness concurrent with engine system development programs. The most critical component for each engine is identified and some of the material problem areas are discussed

Vesicle coating and uncoating: controlling the formation of large COPII-coated carriers

The basic mechanisms underlying the formation of coated vesicles are now defined in considerable detail. This article highlights recent developments in our understanding of the problem of exporting large macromolecular cargo such as procollagen from the endoplasmic reticulum and discusses the implications that this has for cell and tissue organisation and human disease

Test of Nuclear Wave Functions for Pseudospin Symmetry

Using the fact that pseudospin is an approximate symmetry of the Dirac Hamiltonian with realistic scalar and vector mean fields, we derive the wave functions of the pseudospin partners of eigenstates of a realistic Dirac Hamiltonian and compare these wave functions with the wave functions of the Dirac eigenstates.Comment: 11 pages, 4 figures, minor changes in text and figures to conform with PRL requirement