4 research outputs found

    Additional file 2: of Effect of neoadjuvant chemotherapy on the immune microenvironment in non–small cell lung carcinomas as determined by multiplex immunofluorescence and image analysis approaches

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    Figure S2. Characterization of immune cell phenotyping using marker panel 1. Cell phenotypes were defined as malignant cells expressing PD-L1 (AE1/AE3 + PD-L1+), T lymphocytes (CD3; pan T-cell marker including helper T cells CD3 + CD4+, cytotoxic T cells CD3 + CD8+, and other CD3+ T cells), helper T cells (CD3 + CD4+), cytotoxic T cells (CD3 + CD8+), tumor-associated macrophages (TAMs; CD68+), and TAMs expressing PD-L1 (CD68 + PD-L1+). Each type of cell is shown as individual images by marker and by a composite image. (PPTX 102487 kb

    Additional file 3: of Effect of neoadjuvant chemotherapy on the immune microenvironment in non–small cell lung carcinomas as determined by multiplex immunofluorescence and image analysis approaches

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    Figure S3. Characterization of immune cell phenotyping using marker panel 2. Cell phenotypes were defined as memory cells (CD45RO, including memory/natural killer cells CD45RO + CD57 + granzyme B−, memory/regulatory cells CD45RO + FOXP3+, memory antigen experienced cells CD45RO + PD-1+, and other CD45RO+ cells), memory/regulatory cells (CD45RO + FOXP3+), memory antigen experienced cells (CD45RO + PD-1+), activated natural killer cells (CD57 + granzyme B + CD45RO−), and antigen experienced cells (PD-1, including PD-1 + CD45RO+ and other PD-1+ cells). Each type of cell is shown as individual images by marker and by a composite image. (PPTX 103717 kb
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