De las desigualidades socio-ecológicas a la desigual distribución de vulnerabilidades a desastres

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Additional file 17: Figure S9. of The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp

The terpenoid biosynthesis pathway with enzymes encoded in the P. brassicae genome coloured green. (PNG 823 kb

A. Schematic of a toolbox to dissect microbial community structure and their functional genes in a complex community.

<p>The culture-independent SMB toolbox comprises stable isotope probing, metagenomic sequencing and biosensor-based gene transducer. <b>B. </b><b>Schematic of the biosensor-based gene transducer.</b> This approach can be used to screen for gene encoded functions for the metabolism of molecules with no distinguishable activity or phenotypes.</p

Naphthalene dioxygenase (NDO) PCR products.

<p>Two pairs of degenerate primers, COM1_F&COM1_R (<i>Comamonas</i>-type), and PSE1_F&PSE1_R (<i>Pseudomonas</i>-type) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047530#pone-0047530-t001" target="_blank">Table 1</a>) and different DNA templates: 1, 2 - ¹³C-DNA; 3, 4 - ¹²C-DNA; 5– blank control were used. M - DNA molecular weight Ladder.</p

Structure of the <i>nag</i> operons revealed by BGT and pyrosequencing.

<p>The <i>nag2</i> operon was of a mosaic-type pattern. The gray scale of individual genes within the <i>nag2</i> operon indicates % similarity to <i>nag</i>-U2 (black) or <i>nag</i>-CJ2 (white). The numbers beneath the <i>nag</i>2 operon indicates the % similarity to <i>nag</i>-U2/<i>nag</i>-CJ2/neither.</p

Primers used in this study.

<p>Primers used in this study.</p

Strains and plasmids used in this study.

<p>Strains and plasmids used in this study.</p

Taxonomic assignments of the microbial communities in the ¹²C- and ¹³C-DNA fractions revealed by pyrosequencing.

<p>Relative abundance of the species as a % of total 16S-rRNA reads is shown.</p

Bioluminescence of biosensor transducers incorporating DHN degradation genes.

<p>ADPWH_Nag containing a <i>nag</i>2 gene cluster was rapidly activated to show bioluminescence after the addition of 50 µM DHN, confirming the function of the gene cluster. No bioluminescence was detected in the absence DHN. The negative control ADPWH_1274 did not respond to DHN. ADPWH_Nah containing a <i>nah</i> gene cluster was used as a positive control.</p